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Recombinant Human IGFBP-4 Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human IGFBP-4 Protein, CF Summary

Details of Functionality
Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 0.03-0.09 µg/mL in the presence of 14 ng/mL recombinant human IGF-II.
Source
Mouse myeloma cell line, NS0-derived human IGFBP-4 protein
Asp22-Glu258, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IGFBP4
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
32 kDa, reducing conditions
Publications
Read Publications using
804-GB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGFBP-4 Protein, CF

  • BP-4
  • HT29-IGFBP
  • IBP4insulin-like growth factor-binding protein 4
  • IGF-binding protein 4
  • IGFBP4
  • IGFBP-4
  • IGFBP-4IBP-4
  • insulin-like growth factor binding protein 4

Background

IGFBP-4 (insulin-like growth factor binding protein 4) is member of the IGFBP family of structurally similar secreted glycoproteins that maintain IGF-I and IGF-II in the circulation and direct them to their target tissues (1, 2). Human IGFBP-4 cDNA encodes 258 amino acids (aa) that include a 21 aa signal peptide and a 237 aa cysteine-rich mature protein that contains an N-terminal IGFBP domain, a linker sequence, and a C-terminal thyroglobulin domain. Mature human IGFBP-4 shares 91% aa sequence identity with mouse and rat IGFBP-4, and 97% with canine, bovine, ovine and porcine IGFBP-4. Both domains interact with IGFs, although the N‑terminal domain is primary (2). The linker contains a cleavage site that is accessible by PAPP-A (pregnancy-associated plasma protein A) when IGFs are bound (2, 3). IGFBP-4 fragments detected in the plasma of pregnant women have lowered affinity for IGFs, thus upregulating IGF bioavailability for receptor binding (2). Unlike other family members, IGFBP-4 does not interact with the extracellular matrix or translocate to the nucleus (2). Like other family members, it is expressed early in development and has both IGF-dependent and -independent activities (2, 4). IGFBP-4 expression in mesenchymal stem cells is upregulated by the cardiac transcription factor GATA-4 (5). It then enhances cardiomyocyte differentiation by interacting with Frizzled and LRP proteins, inhibiting canonical Wnt signaling (6, 7). IGF can sequester IGFBP-4 when present, inhibiting its ability to support cardiomyocyte differentiation (3, 4). IGFBP-4 is also upregulated in osteoblasts in response to estrogen (8). Intact or proteolytically cleaved IGFBP-4 may either promote or inhibit cancer cell growth, motility and invasion depending on cell type, tissue and stage of the cancer (9-11). It is shown to inhibit IGF-1- or FGF-2-induced angiogenesis, but not that induced by VEGF (10).

  1. Bethel, C.R. et al. (1994) Biochem. Mol. Biol. Int. 34:385.
  2. Forbes, B.E. et al. (2012) Front. Endocrinol. (Lausanne) 3:38.
  3. Gyrup, C. and C. Oxvig  (2007) Biochemistry 46:1972.
  4. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  5. Li, H. et al. (2011) Cytotherapy 13:1057.
  6. Zhu, W. et al. (2008) Nature 454:345.
  7. Minato, A. et al. (2012) Biomaterials 33:515.
  8. Denger, S. et al. (2008) Mol. Endocrinol. 22:361.
  9. Ueno, K. et al. (2011) Int. J. Cancer 129:2360.
  10. Contois, L.W. et al. (2012) J. Biol. Chem. 287:1779.
  11. Moreno, M.J. et al. (2013) Neoplasia 15:554.

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Publications for IGFBP-4 (804-GB)(9)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: Bioassay, ELISA (Standard), Enzyme Assay, Western Blot.


Filter By Application
Bioassay
(6)
ELISA (Standard)
(1)
Enzyme Assay
(2)
Western Blot
(1)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 9 of 9.
Publications using 804-GB Applications Species
RA Judge, J Sridar, K Tunyasuvun, R Jain, JCK Wang, C Ouch, J Xu, A Mafi, AH Nile, C Remarcik, CL Smith, C Ghosh, C Xu, V Stoll, J Jumper, AH Singh, D Eaton, Q Hao Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition Nature Communications, 2022-09-20;13(1):5500. 2022-09-20 [PMID: 36127359] (Bioassay, Human) Bioassay Human
M Mohrin, J Liu, J Zavala-Sol, S Bhargava, J Maxwell Tr, A Brito, D Hu, D Brooks, G Koukos, L Alabdulaal, JS Paw, K Hake, G Kolumam, ML Bouxsein, R Baron, Y Kutskova, A Freund Inhibition of longevity regulator PAPP-A modulates tissue homeostasis via restraint of mesenchymal stromal cells Aging Cell, 2021-02-09;0(0):e13313. 2021-02-09 [PMID: 33561324] (Bioassay, Human) Bioassay Human
E Wirthgen, S Goumon, M Kunze, C Walz, M Spitschak, A Tuchschere, J Brown, C Höflich, L Faucitano, A Hoeflich Effects of Transport Duration and Environmental Conditions in Winter or Summer on the Concentrations of Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins in the Plasma of Market-Weight Pigs Front Endocrinol (Lausanne), 2018-02-13;9(0):36. 2018-02-13 [PMID: 29487569] (Western Blot, Human) Western Blot Human
Crosley , Erin J, Dunk , Caroline, Beristain , Alexande, Christians , Julian K IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells. Reprod Biol Endocrinol, 2014-12-04;12(0):123. 2014-12-04 [PMID: 25475528] (Bioassay, Human) Bioassay Human
Eckstein N, Servan K, Hildebrandt B, Politz A, von Jonquieres G, Wolf-Kummeth S, Napierski I, Hamacher A, Kassack MU, Budczies J, Beier M, Dietel M, Royer-Pokora B, Denkert C, Royer HD Hyperactivation of the insulin-like growth factor receptor I signaling pathway is an essential event for cisplatin resistance of ovarian cancer cells. Cancer Res., 2009-03-24;69(7):2996-3003. 2009-03-24 [PMID: 19318572] (Bioassay, Human) Bioassay Human
Wang TH, Chang CL, Wu HM, Chiu YM, Chen CK, Wang HS Insulin-like growth factor-II (IGF-II), IGF-binding protein-3 (IGFBP-3), and IGFBP-4 in follicular fluid are associated with oocyte maturation and embryo development. Fertil. Steril., 2006-11-01;86(5):1392-401. 2006-11-01 [PMID: 17070193] (Bioassay, Human) Bioassay Human
Cortesio CL, Jiang W Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5. Arch. Biochem. Biophys., 2006-03-03;449(1):164-70. 2006-03-03 [PMID: 16554018] (Enzyme Assay, Human) Enzyme Assay Human
Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA Steroids and protein markers in the follicular fluid as indicators of oocyte quality in patients with and without endometriosis. J. Assist. Reprod. Genet., 2005-06-01;22(6):257-64. 2005-06-01 [PMID: 16021855] (ELISA (Standard)) ELISA (Standard)
Nakamura M, Miyamoto S, Maeda H, Ishii G, Hasebe T, Chiba T, Asaka M, Ochiai A Matrix metalloproteinase-7 degrades all insulin-like growth factor binding proteins and facilitates insulin-like growth factor bioavailability. Biochem. Biophys. Res. Commun., 2005-08-05;333(3):1011-6. 2005-08-05 [PMID: 15964556] (Bioassay, Enzyme Assay, Human) Bioassay, Enzyme Assay Human

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Bioinformatics

Gene Symbol IGFBP4
Uniprot