![SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-IgG-Fc-Native-Protein-SDS-Page-NBP1-97061-img0003.jpg "SDS-Page: Rabbit IgG, Fc Native Protein [NBP1-97061] - SDS-PAGE of Rabbit IgG F(c) Fragment. Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.")
| Reactivity | RbSpecies Glossary |
| Applications | WB, ELISA, IHC, PAGE |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97231 | |||
| FITC | NBP1-97227 | |||
| HRP | NBP1-97241 | |||
| Description | Native Rabbit Rabbit IgG, Fc Source: Normal serum (Accession #:NP_000079.2) Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Rabbit IgG F(c) fragment is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. |
| Preparation Method |
This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(c). No reaction was observed against anti-Rabbit IgG F(ab')2 or anti-Papain. |
| Protein/Peptide Type | Native Protein |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
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