PPP4R4 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

The protein encoded by the PPP4R4 gene is a HEAT-like repeat-containing protein. The HEAT repeat is a tandemly repeated,37-47 amino acid long module occurring in a number of cytoplasmic proteins. Arrays of HEAT repeats form a rod-likehelical structure and appear to function as protein-protein interaction surfaces. The repeat-containing region of thisprotein has some similarity to the constant regulatory domain of the protein phosphatase 2A PR65/A subunit. Thefunction of this particular gene product has not been determined. Alternative splicing has been observed for this geneand two transcript variants encoding distinct isoforms have been identified. (provided by RefSeq)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Array NBL1-12274

• PPP4R4 Antibodies
• PPP4R4 Lysates
• PPP4R4 Proteins