PPAP2C Recombinant Protein Antigen Summary
Description |
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PPAP2C. Source: E. coli
Amino Acid Sequence: PQHCLKEEELERKPSLSLTLTLGEADHNHYGYPHSS Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)
This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions. |
Source |
E. coli |
Protein/Peptide Type |
Recombinant Protein Antigen |
Gene |
PLPP2 |
Purity |
>80% by SDS-PAGE and Coomassie blue staining |
Applications/Dilutions
Dilutions |
- Antibody Competition 10 - 100 molar excess
|
Application Notes |
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-31607. It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml. For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com |
Theoretical MW |
22 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Packaging, Storage & Formulations
Storage |
Store at -20C. Avoid freeze-thaw cycles. |
Buffer |
PBS and 1M Urea, pH 7.4. |
Preservative |
No Preservative |
Purity |
>80% by SDS-PAGE and Coomassie blue staining |
Alternate Names for PPAP2C Recombinant Protein Antigen
Background
PPAP2C, also referred to as Phosphatidic Acid Phosphatase Type 2C, has a canonical sequence that is 288 amino acids long and approximately 32kDa, and two other isoforms that are 309 and 232 amino acids in length, respectively. PPAP2C is an enzyme that hydrolyzes S1P, C1P and LPA, and catalyzes the conversion of phosphatidic acid to diacylglycerol. Current research surrounding PPAP2C has shown a possible linkage with strabismus and gastroenteritis. PPAP2C has also been shown to interact with APLP1, BAG6, GALC, MLH1, and UNC119 in pathways such as triacylglyceride synthesis, sphingolipid metabolism, triacylglycerol biosynthesis, and Fc gamma R-mediated phagocytosis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are
guaranteed for 3 months from date of receipt.
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