![Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit](http://images.novusbio.com/fullsize/Polyinosinic-polycytidylic-acid-NBP2-25288-img0002.jpg "Polyinosinic-polycytidylic acid [NBP2-25288] - 293T cells were co-transfected with pCMV/TLR3 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000 (Life Technology). After 48 hrs of transfection, 10 ug/ml of Poly(I:C) was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using the NF-kB SEAPorter Assay kit")
| Description | Polyinosinic-polycytidylic acid (Poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA) that is produced during the replicative life cycle of a majority of viruses. Poly(I:C) is known to induce type-1 interferon (IFN) through TLR3. Poly(I:C) directly binds to TLR3. |
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| Application Notes | Used for activation of TLR3. Stimulation of TLR3 can be achieved with 10-100 ug/ml. Use in In vitro assay reported in scientific literature (PMID 24743304) .Use in Ligand activation, and functional reported in scientific literature (PMID 25957979). Use in In vivo assay reported in scientific literature (PMID 26196739). Use in Ligand Activation reported in scientific literature (PMID 26503951) |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | This is supplied in sterile low endotoxin water (<0.05 EU/ug) at 1 mg/ml |
| Concentration | Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance. |
