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pCL-10A1 Retrovirus Packaging Vector

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Product Details

Summary
Reactivity Mu, MaSpecies Glossary
Applications NULL
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

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pCL-10A1 Retrovirus Packaging Vector Summary

Specificity
Ecotropic (MoMuLV) for mouse and rat cells only (not human)
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster)
10A1 (MuLV) for most mammalian cells (including hamster)

Applications/Dilutions

Dilutions
  • Retroviral Production
Application Notes
AMPLIFICATION: The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml. Use in retroviral production reported in multiple pieces of scientific literature.
Publications
Read Publications using
NBP2-29542 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:33168978) Human reactivity reported in scientific literature (PMID: 23973628)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10 ug in 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

Alternate Names for pCL-10A1 Retrovirus Packaging Vector

  • pcl 10a1 retrovirus packaging vector
  • pCL-10A1 Retrovirus
  • pCL10A1
  • pCL-10A1

Background

The pCL-10A1 packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system. By introducing a retroviral vector into a cell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of at the rate of about 1 infectious particle/cell/day Retrovirus tropism is determined at 3 levels. The first is simply a function of viral envelope protein, gp70. The envelope determines which cells the virus will enter. pCL-10A1 expresses 10A1 envelope protein that was isolated from a mouse infected with amphotropic MuLV (2,3). Retroviruses obtained by cotransfection with pCL-10A1 vector will infect most mammalian cells including hamster cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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Publications for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542)(55)

We have publications tested in 3 confirmed species: Human, Mouse, Mammal.

We have publications tested in 3 applications: IHC-P, Ret Vir, RetVir.


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IHC-P
(1)
Ret Vir
(3)
RetVir
(14)
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Human
(13)
Mouse
(1)
Mammal
(1)
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Showing Publications 1 - 10 of 55. Show All 55 Publications.
Publications using NBP2-29542 Applications Species
Miyauchi S, Kim SS, Jones RN et al. Human papillomavirus E5 suppresses immunity via inhibition of the immunoproteasome and STING pathway Cell reports 2023-05-11 [PMID: 37171962]
Li JSZ, Abbasi A, Kim DH et al. Chromosomal fragile site breakage by EBV-encoded EBNA1 at clustered repeats Nature 2023-04-01 [PMID: 37046091]
Ahn CH, Oh KY, Jin B et al. Targeting tumor-intrinsic PD-L1 suppresses the progression and aggressiveness of head and neck cancer by inhibiting GSK3 beta -dependent Snail degradation Cellular oncology (Dordrecht) 2022-11-28 [PMID: 36441378]
Kim M, Singh M, Lee BK et al. A MYC-ZNF148-ID1/3 regulatory axis modulating cancer stem cell traits in aggressive breast cancer Oncogenesis 2022-10-07 [PMID: 36207293] (RetVir) RetVir
Xie X, Gan T, Rao B et al. C-terminal deletion-induced condensation sequesters AID from IgH targets in immunodeficiency The EMBO journal 2022-04-26 [PMID: 35471583] (RetVir) RetVir
Ma L, Li W, Zhang Y Et al. FLT4/VEGFR3 activates AMPK to coordinate glycometabolic reprogramming with autophagy and inflammasome activation for bacterial elimination Autophagy 2021-10-10 [PMID: 34632918]
Hussain S, Tran T, Ware TB et al. RalA and PLD1 Promote Lipid Droplet Growth in Response to Nutrient Withdrawal Cell Rep 2021-07-28 [PMID: 34320341]
Shin JA, Kim LH, Ryu MH et al. Withaferin A mitigates metastatic traits in human oral squamous cell carcinoma caused by aberrant claudin-1 expression Cell biology and toxicology 2021-03-04 [PMID: 33665778]
Guarnaccia AD, Rose KL, Wang J, et al. Impact of WIN site inhibitor on the WDR5 interactome Cell reports 2021-01-19 [PMID: 33472061]
Kobayashi H, Hatakeyama H, Nishimura H et al. Chemical reversal of abnormalities in cells carrying mitochondrial DNA mutations Prog Neurobiol 2020-11-03 [PMID: 33168978] (IHC-P, Mouse) IHC-P Mouse
Show All 55 Publications.

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FAQs for pCL-10A1 Retrovirus Packaging Vector (NBP2-29542). (Showing 1 - 3 of 3 FAQs).

  1. I am having a retroviral system in place which allows me to infect mouse cell lines. The vectors I have are MIG.R (with my gene of interest), pCL-ECO and VSV-g. I know that my pCL-ECO vector is only suitable for infecting mouse cells. My question is: Is it possible to use my MIG.R and VSV-g vectors, and exchange the mouse specific pCL-ECO with pCL-Ampho or pCL-10A1 packaging vector which you both offer for purchase (Cat Numbers are 10045P, 10046P, 10047P). My aim is to infect human cell lines with my gene of interest.
    • These vectors, while also sold separately, are components of the RetroMax system 10040K. The vectors are designed to be used as described in the 10040K manual. Yes, you are correct, pCL-ECO is for mouse and rat, and not for human. We have not evaluated the Retromax system with the MIG.R and VSV-g vectors, and can not promise they would be compatible with your vectors. The Retromax packaging vectors are designed to be used with the expression vectors in the Retromax Kit. However, the packaging vectors are widely published and you may be able to find some information in the literature relevant to your model system (search scholar.google.com and keywords with Retromax and vector name, or keys words with packaging vector name and expression vector name ).
  2. Can you please let me know if NBP2-29542 contain the Retrovirus ?
    • The pCL-10A1 Retrovirus Packaging Vector NBP2-29542 does not actually contain the retrovirus. The vector is just the DNA plasmid.
  3. Can pCL-10A1 Retrovirus Packaging vector (NBP2-29542) be used with Lentivirus shRNA vector pGLV3?
    • When co-transfecting/transducing pCL-10A1 with a pCLX/N expression vector in the acell line, pCL-10A1 will produce retrovial Gag, Pol, and Env proteins for packing the pCLX/N-expressed viral RNA genome.  [pCLX/N expression vectors have both Amp and Neo genes for growing in E.coli and stably transfection in a mammalian cell line, respectively]A key feature for appropriate packaging into viral protein shells is the packaging signal(s)  UR5 on the viral RNA genome, which is derived from one of pCLX/N expression vectors. Check the nucleotide sequence(s) for the packaging signal(s) on your plasmid and compare to established UR5 sequence(s) to asses the likelihood of co-transduction experiments using pCL-10A1 and your vector successfully producing infectious virions.

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Product PDFs

  Background and Contents -pCL-10A1 Retrovirus Packaging Vector
  Manual - Retroviral Expression System
  NBP2-29542 - Protocol
  Product Handling Protocol - pCL-10A1 Retrovirus Packaging Vector
  Protocol - pCL-10A1 Retrovirus Packaging Vector
  PDF Datasheet
  Request CofA

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