OLLAS Epitope Tag Antibody (L2) [FITC] Summary
| Immunogen |
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin |
| Isotype |
IgG1 Kappa |
| Clonality |
Monoclonal |
| Host |
Rat |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation Sequencing
- Immunoblotting
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry Whole-Mount
- Immunohistochemistry
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
- Immunoprecipitation
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
PBS |
| Preservative |
0.05% Sodium Azide |
| Concentration |
1.0 mg/ml |
| Purity |
Protein G purified |
Notes
This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for OLLAS Epitope Tag Antibody (L2) [FITC]
Background
OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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FAQs for OLLAS Epitope Tag Antibody (NBP1-06713F) (0)
Secondary Antibodies
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Isotype Controls
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Blogs on OLLAS Epitope Tag
![Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.](https://images.novusbio.com/images/OLLAS-Epitope-Tag-Antibody-L2-Biotin-Western-Blot-NBP1-06713B-img0002.jpg "Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.")