Store at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Preparation Method
This product was prepared from normal serum by a multi-step process which includes delipidation and selective precipitation. Assay by immunoelectrophoresis resulted in a multiple precipitin arcs against anti-Goat Serum. Normal Goat Serum was obtained from non-immunized healthy goats.
Host
Goat
Purity
Multi-step
Applications/Dilutions
Dilutions
ELISA
Flow Cytometry 1:10 - 1:1000
Fluorophore-linked immunosorbent assay
Immunocytochemistry/ Immunofluorescence
Immunohistochemistry
Immunohistochemistry-Paraffin
Immunoprecipitation
Western Blot
Application Notes
This product has been tested by SDS-PAGE and is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.
Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID: 26004262)
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide, 0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute in 10 ml with deionized water.
Alternate Names for Normal Goat Serum
10% NGS
blocking goat serum
blocking grade
NGS
Background
Goat Serum blocking reagent or NGS is ideal for blocking procedures such as Western Blotting, ELISA and immunochemistry to prevent nonspecific binding.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for Normal Goat Serum (NBP2-23475)(15)
We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.
