Map17 Recombinant Protein Antigen

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications AC

Order Details

Map17 Recombinant Protein Antigen Summary

Description
A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human PDZK1IP1.

Source: E. coli

Amino Acid Sequence: QEEPEPAHMILTVGNKADGVLVGTDGRYSSMAASFRSSEHENAYENVPEEEGKVRST

Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

Source
E. coli
Protein/Peptide Type
Recombinant Protein Antigen
Gene
PDZK1IP1
Purity
>80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

Dilutions
  • Antibody Competition 10 - 100 molar excess
Application Notes
This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP1-84290.

It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS and 1M Urea, pH 7.4.
Preservative
No Preservative
Purity
>80% by SDS-PAGE and Coomassie blue staining

Alternate Names for Map17 Recombinant Protein Antigen

  • 17 kDa membrane-associated protein
  • DD96
  • MAP17epithelial protein up-regulated in carcinoma, membrane associated protein 17
  • membrane-associated protein 17
  • PDZK1 interacting protein 1
  • PDZK1-interacting protein 1
  • Protein DD96
  • RP1-18D14.5
  • SPAP

Background

Map17, also known as small PDZK1-associated protein (SPAP) is an endogenous regulator of cellular PDZK1 levels through the regulation of PDZK1 turnover. MAP17 is only expressed at significant levels in the proximal tubular epithelial cells of the kidney, and is diffusely expressed in various carcinomas originating from kidney, colon, lung and breast. MAP17 is thought to be associates with tumor formation, and MAP17 antibodies are useful for cancer research and protein turnover studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PDZK1IP1