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Human Lung Whole Tissue Lysate (Adult Whole Normal)

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 (1 Review)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Concentration
5.0 mg/ml

Order Details

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Human Lung Whole Tissue Lysate (Adult Whole Normal) Summary

Specificity
Total Protein - Human Adult Normal Tissue: Lung: Left Upper Lobe

Applications/Dilutions

Dilutions
  • Immunoprecipitation
  • Western Blot
Application Notes
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression
Reviewed Applications
Read 1 Review rated 1
using
NB820-59237 in the following applications:
Publications
Read Publications using NB820-59237.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors
Concentration
5.0 mg/ml

Lysate Details for Array

Type
Tissue
Life Stage
Adult
Tissue
Respiratory
Protein State
Native
Tissue Condition
Normal
Subcellular Fraction
Whole

Background

Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for Lung Lysate (NB820-59237)(2)

We have publications tested in 1 application: WB.


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WB
(1)
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Review for Lung Lysate (NB820-59237) (1) 11

Average Rating: 1
(Based on 1 review)

Reviews using NB820-59237:
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Images Ratings Applications Species Date Details
  1
reviewed by:
Ming Lu
 05/15/2024
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Summary

LotB912203

Comments

Comments***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lung Lysate (NB820-59237). (Showing 1 - 1 of 1 FAQs).

  1. For NB820-59237 lysate, I'd like to know the extraction procedure. Which buffer has been used? Protease, Phosphotase Inhibitors? And how will the lysate be delivered (which buffer and concentration)?
    • A total protein extraction kit was used for the extraction of the total protein lysate. This kit uses a TM Buffer (HEPES (pH7.9), MgCl2, Kcl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, Sodium OrthoVanadate) and a cocktail of protease inhibitors. The protein is delivered at 5 ug/ul concentration. We have protease inhibitor cocktail in the lysate, but we don't release the specific protease inhibitor information.

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Product PDFs

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Calculators

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Lung NB820-59237
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 Ming Lu
05/15/2024
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