![Functional: Luciferase Assay Kit [KA1640] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.](http://images.novusbio.com/fullsize/Luciferase-Assay-Kit-Functional-KA1640-img0001.jpg "Functional: Luciferase Assay Kit [KA1640] - The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.")
| Applications | Func |
| Suitable Sample Type | Cells etc |
| Standard Curve Range | We found that as few as 4 cells could be detected with HEK293, and the kit was linear up until 80,000 cells per 96 well. |
| Sensitivity | 2 fg luciferase |
| Description | Quality control test: Standard curve Luciferase Reporter Gene Assay Kit is a bioluminescent assay for promoter regulated luciferase expression. |
| Standard Curve Range | We found that as few as 4 cells could be detected with HEK293, and the kit was linear up until 80,000 cells per 96 well. |
| Sensitivity | 2 fg luciferase |
| Assay Type | Luminescent |
| Sample Volume | 20 uL |
| Kit Type | Assay Kit (Luminescent) |
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| Storage | Storage of components varies. See protocol for specific instructions. |
| Publication using KA1640 | Applications | Species |
|---|---|---|
| Drolia R, Tenguria S, Durkes AC et al Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation. Cell Host Microbe. 2019-04-05 [PMID: 29606495] (Func) | Func |
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Luciferase: Shining a Light to See Inside Living Animal Models The luciferase reporter is a valuable tool for research into physiology and disease. Light emitted from luciferase enables the monitoring of xenografted tumors, specific cell types, gene expression and pathogens within live animals over time using bio... Read full blog post. |
![Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.](https://images.novusbio.com/images/Luciferase-Antibody-Luci-21-1-107-Western-Blot-NB600-307-img0006.jpg "Western Blot: Luciferase Antibody (Luci 21 1-107) [NB600-307] - Reduction in CREB activity in flies following DN-CREB expression in the fat body. CRE-Luciferase reporter protein was measured using anti-luciferase antibody in Western blots of body extracts from control flies (to-GAL4 driver only, control) or flies expressing DN-CREB in the fat body from the to-GAL4 driver (DN-CREB) (top panel). Blots were stripped and reprobed with anti-tubulin antibodies as a protein loading control (bottom panel). Signal intensities were quantified and are shown as ratios to control signals (mean+/-SD, n = 5; *p<0.05, Student's t-test). Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0008498) licensed under a CC-BY license.")