Luciferase Antibody

Luciferase is a generic term for a group of oxidative enzymes used in bioluminescence. Firefly (Photinus pyralis) and bacterial luciferase enzymes are commonly used in assay systems such as cell viability assays, reporter gene assays, and for in vivo imaging. Bacterial luciferases are flavoenzymes composed of two subunits each encoded by the luxA and luxB genes, while the firefly luciferase is a single polypeptide specified by the luc gene (1). Firefly luciferase (theoretical molecular weight: 61 kDa) oxidizes the substrate luciferin to oxyluciferin in a bioluminescent reaction requiring Mg2+ and ATP (2,3). This reaction produces a flash of yellow-green light with an emission peak around 560nm that can be detected by a luminometer (3). Firefly luciferase has become one of the more widely used reporter proteins and is an excellent tool for the study of gene expression, given that the amount of light emitted is directly proportional to luciferase activity (4).

The luciferase assay is fast and sensitive, differentiating itself from the CAT (chloramphenicol acetyltransferase) assay because it does not require a radioactive substrate.

References

1. Eun, H. (1996). Marker/Reporter enzymes. Enzymology Primer for Recombinant DNA Technology, 567-645. doi:10.1016/b978-012243740-3/50011-9

2. McNabb, D. S., Reed, R., & Marciniak, R. A. (2005). Dual luciferase assay system for rapid assessment of gene expression in Saccharomyces cerevisiae. Eukaryotic Cell, 4(9), 1539-1549. doi:10.1128/ec.4.9.1539-1549.2005

3. Fraga, H. (2008). Firefly luminescence: A historical perspective and recent developments. Photochemical & Photobiological Sciences, 7(2), 146-158. doi:10.1039/b719181b

4. Younes, A., Lukyanenko, Y. O., Lyashkov, A. E., Lakatta, E. G., & Sollott, S. J. (2011). A bioluminescence method for direct measurement of phosphodiesterase activity. Analytical Biochemistry, 417(1), 36-40. doi:10.1016/j.ab.2011.05.036

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Firefly.

We have publications tested in 12 applications: Block/Neutralize, ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-P, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, MiAr, WB, Western Blot.

Showing Publications 1 - 10 of 31. Show All 31 Publications. Collapse Publications.

Publications using NB100-1677	Applications	Species
Rogg M, Maier JI, Helmstädter M et al. A YAP/TAZ-ARHGAP29-RhoA Signaling Axis Regulates Podocyte Protrusions and Integrin Adhesions Cells 2023-07-06 [PMID: 37443829] (WB) Details: Dilutions: 1:1000	WB
Escudero-Duch C, Mu�oz-Moreno L, Martin-Saavedra F et al. Remote control of transgene expression using noninvasive near-infrared irradiation Journal of photochemistry and photobiology. B, Biology 2023-03-22 [PMID: 36963296] (IHC)	IHC
Melamed JR, Yerneni SS, Arral ML et al. Ionizable lipid nanoparticles deliver mRNA to pancreatic ? cells via macrophage-mediated gene transfer Science Advances 2023-01-27 [PMID: 36706177] (Immunohistochemistry)	Immunohistochemistry
Lekk I, Cabrera-Cabrera F, Turconi G et al. Untranslated regions of brain-derived neurotrophic factor mRNA control its translatability and subcellular localization Journal of Biological Chemistry 2023-02-01 [PMID: 36639028] (Immunoprecipitation, Western Blot, Block/Neutralize)	Immunoprecipitation, Western Blot, Block/Neutralize
Morita A, Nakayama M, Wang D et al. Frequent loss of metastatic ability in subclones of Apc, Kras, Tgfbr2, and Trp53 mutant intestinal tumor organoids Cancer science 2023-04-01 [PMID: 36576236] (IHC-P)	IHC-P
Lee SY, Oh HR, Kim YH et al. Cerenkov luminescence imaging of interscapular brown adipose tissue using a TSPO-targeting PET probe in the UCP1 ThermoMouse Theranostics 2022-08-29 [PMID: 36168637] (Immunohistochemistry)	Immunohistochemistry
Sullivan PM, Kumar R, Li W et al. FGFR4-Targeted Chimeric Antigen Receptors Combined with Anti-Myeloid Polypharmacy Effectively Treat Orthotopic Rhabdomyosarcoma Molecular Cancer Therapeutics 2022-10-07 [PMID: 35877472] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Trieu KG, Tsai SY, Eberl M et al. Basal cell carcinomas acquire secondary mutations to overcome dormancy and progress from microscopic to macroscopic disease Cell reports 2022-05-03 [PMID: 35508126] (ICC/IF)	ICC/IF
Lu X, Fong KW, Gritsina G et al. HOXB13 suppresses de novo lipogenesis through HDAC3-mediated epigenetic reprogramming in prostate cancer Nature genetics [PMID: 35468964] (MiAr)	MiAr
Noffsinger B, Witter A, Sheybani N Et al. Technical choices significantly alter the adaptive immune response against immunocompetent murine gliomas in a model-dependent manner Journal of neuro-oncology 2021-08-25 [PMID: 34432197]
Gutierrez S, Eisenach Jc, Boada Md Seeding of breast cancer cell line (MDA-MB-231LUC+) to the mandible induces overexpression of substance P and CGRP throughout the trigeminal ganglion and widespread peripheral sensory neuropathy throughout all three of its divisions Molecular pain 2021-07-07 [PMID: 34229504] (IHC-Fr)	IHC-Fr
Lazaro-Ibanez E, Faruqu FN, Saleh AF et al. Selection of Fluorescent, Bioluminescent, and Radioactive Tracers to Accurately Reflect Extracellular Vesicle Biodistribution in Vivo ACS Nano 2021-01-20 [PMID: 33470092] (WB)	WB
Shaik S, Maegawa S, Haltom AR et al. REST promotes ETS1-dependent vascular growth in medulloblastoma Molecular Oncology 2021-05-01 [PMID: 33469989] (Immunohistochemistry, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
Kang MH, van Lieshout LP, Xu L et al. A lung tropic AAV vector improves survival in a mouse model of surfactant B deficiency Nat Commun 2020-08-09 [PMID: 32764559] (ICC/IF)	ICC/IF
Garg S, Wu C et al. Express ELISA for detection of mortalin. Biotechniques 2019-01-10 [PMID: 31502469] (ICC/IF)	ICC/IF
Chen TH, Arra M, Mbalaviele G, Swarnkar G, Abu-Amer Y. Inflammatory Responses Reprogram T(REGS) Through Impairment of Neuropilin-1 Sci Rep 2019-07-20 [PMID: 31320680] (IHC-P)	IHC-P
Ohta Y, Nomura E, Shang J, et al. Enhanced oxidative stress and the treatment by edaravone in mice model of amyotrophic lateral sclerosis J Neurosci Res 2018-12-20 [PMID: 30565312] (ICC/IF)	ICC/IF
Singh RD, Hillestad ML, Livia C, et al. M(3)RNA Drives Targeted Gene Delivery in Acute Myocardial Infarction Tissue Eng Part A 2018-07-27 [PMID: 30047313] (ICC/IF)	ICC/IF
Henrich MT, Geibl FF, Lee B et al. A53T-a-synuclein overexpression in murine locus coeruleus induces Parkinson's disease-like pathology in neurons and glia Acta Neuropathol Commun 2018-05-10 [PMID: 29747690] (ICC/IF, Firefly)	ICC/IF	Firefly
Vadrot N, Duband-Goulet I, Cabet E, et al. The p.R482W substitution in A-type lamins deregulates SREBP1 activity in Dunnigan-type familial partial lipodystrophy Hum Mol Genet 2014-12-20 [PMID: 25524705] (ICC/IF)	ICC/IF
Cho H, Herzka T, Zheng W, et al. RapidCaP, a novel GEM model for metastatic prostate cancer analysis and therapy, reveals myc as a driver of Pten-mutant metastasis Cancer Discov 2014-01-22 [PMID: 24444712] (IHC-P)	IHC-P
Ozdemir M, Attar A, Kuzu I, et al. Stem cell therapy in spinal cord injury: in vivo and postmortem tracking of bone marrow mononuclear or mesenchymal stem cells Stem Cell Rev Rep 2012-05-04 [PMID: 22552878] (IHC-Fr, ICC/IF)	IHC-Fr, ICC/IF
Yang X, Balakrishnan I, Torok-Storb B, et al. Marrow Stromal Cell Infusion Rescues Hematopoiesis in Lethally Irradiated Mice despite Rapid Clearance after Infusion Adv Hematol 2012-03-09 [PMID: 22400029] (IF/IHC)	IF/IHC
Kawabata A, Baoum A, Ohta N, et al. Intratracheal administration of a nanoparticle-based therapy with the angiotensin II type 2 receptor gene attenuates lung cancer growth Cancer Res 2012-03-06 [PMID: 22389453] (IF/IHC)	IF/IHC
Dewals B, Myster F, Palmeira L et al. Ex vivo bioluminescence detection of alcelaphine herpesvirus 1 infection during malignant catarrhal fever J Virol 2011-07-01 [PMID: 21593175] (ICC/IF)	ICC/IF
Yang G, Abate A, George AG, et al. Maturational differences in lung NF-kappaB activation and their role in tolerance to hyperoxia J Clin Invest 2004-09-03 [PMID: 15343385] (IF/IHC)	IF/IHC
Timmermann L The Locus coeruleus in Parkinson's disease-from basic research to new translational perspectives Thesis (IF/IHC)	IF/IHC
Yun Y, Oh J, Kim Y et al. Characterization of neural stem cells modified with hypoxia/neuron-specific VEGF expression system for spinal cord injury Gene Ther. 2017-11-20 [PMID: 29155421]
Luengo-Gil G, Calvo MI, Martin-Villar E et al. Antithrombin controls tumor migration, invasion and angiogenesis by inhibition of enteropeptidase. Sci Rep 2016-06-09 [PMID: 27270881] (IHC-Fr)	IHC-Fr
Sayed D, He M, Yang Z et al. Transcriptional Regulation Patterns Revealed by High Resolution Chromatin Immunoprecipitation during Cardiac Hypertrophy. J Biol Chem 2013-01-25 [PMID: 23229551] (WB, Firefly)	WB	Firefly
Ozdemir M, Attar A, Kuzu I. Regenerative treatment in spinal cord injury Curr Stem Cell Res Ther 2012-09-01 [PMID: 22849699]
Show All 31 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Yao Wang	ICC	Mouse	12/30/2022	View Summary Application Immunocytochemistry Sample Tested E0771 Species Mouse Lot nt Comments Comments ***Novus Innovators Program - new species or application used on a primary antibody.***
	5 reviewed by: Verified Customer	IHC-P	Human	11/25/2022	View Summary Application Immunohistochemistry-Paraffin Sample Tested Breast cancer cells Species Human Lot 47401
	5 reviewed by: Verified Customer	WB	Mouse	04/05/2018	View Summary Application Western Blot Sample Tested Mouse Bone marrow derived macrophage and osteoclast Species Mouse Lot 35113
Enlarge	5 reviewed by: Alexandra Ambrico	Immunocytochemistry/Immunofluorescence	Mouse	10/10/2016	View Summary Application Immunocytochemistry/Immunofluorescence Sample Tested mouse prostate Species Mouse
Enlarge	5 reviewed by: Verified Customer	IHC-P	Other	06/18/2016	View Summary Application Immunohistochemistry-Paraffin Sample Tested Mouse brain tissue bearing human glioma cells expressing fire fly luciferase Species Other
Enlarge	5 reviewed by: Verified Customer	IHC-P	Other	06/21/2013	View Summary Application Immunohistochemistry-Paraffin Sample Tested Human tumor xenograft expressing firefly luciferase intracranially injected into a mouse brain Species Other Lot 28596
Enlarge	4 reviewed by: Benjamin Dewals	ICC	Other	10/04/2011	View Summary Application Immunocytochemistry Sample Tested virus expressing luciferase transgene Species Other

Array NB100-1677

• Luciferase Antibodies
• Luciferase Kit
• Luciferase Proteins