Reactivity | HuSpecies Glossary |
Applications | Func, HC, IHC |
Description | Frozen tissue slides are ideal for rapid cellular localization of RNA and protein expression, especially for antibodies that don't recognize paraffin embedded tissue. Tissues were snap frozen in liquid nitrogen immediately after excision, and embedded in OCT. Frozen tissue sections in each pair are prepared from the same donor. Tissue sections are 5-10 m in thickness, mounted on positively charged glass slides, and fixed by cold acetone. At least one of the tissue slides from each lot was stained with H & E for quality assurance. Product is supplied as 5 diseased slides and 5 adjacent normal slides. |
Dilutions |
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Application Notes | Each individual tissue spot should be >90% core retention and total core lost from 1 slide should be no more than 5% |
Storage | Store at -70C. Avoid freeze-thaw cycles. |
Signalling Advances in Adiponectin Antibody Research Adiponectin is an adipocytokine protein that positively regulates metabolism of lipids and glucose by suppressing glucose production from the liver, stimulating insulin sensitivity, and increasing the rate of fatty acid oxidation and glucose uptake. I... Read full blog post. |