Home » Lightning-Link® Rapid Type A Biotin » Lightning-Link® Rapid Type A Biotin Kits » Lightning-Link® Rapid Type A Biotin Antibody Labeling Kit
Novus Biologicals products are now on bio-techne.com

Lightning-Link® Rapid Type A Biotin Antibody Labeling Kit

 (5 Publications)
 See All Related
Submit a Review

Images

 
(
Enlarge)
Lightning-Link Rapid Type A Biotin Antibody Labeling Kit [370-0010] - CRP labeled with Lightning-Link Rapid Biotin A at 10ug, 100ug, 1mg, 10mg and 100mg single reaction vials tested with Streptavidin-Rabbit IgG in a sandwich ELISA. Detection: ABTS.
Lightning-Link Rapid Type A Biotin Antibody Labeling Kit [370-0010] - CRP labeled with Lightning-Link Rapid Biotin A at 10ug, 100ug, 1mg, 10mg and 100mg single reaction vials tested with Streptavidin-Rabbit IgG in a ...read more

Product Details

Summary
Conjugate
Biotin
Format
Biotin (A=N/A, E=N/A)
Kit Type
Antibody Labeling Kit

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Novus Biologicals, part of Bio-Techne, offers 100% Guarantee

Lightning-Link® Rapid Type A Biotin Antibody Labeling Kit Summary

Description
Lightning-Link Rapid Antibody Labeling kits enable direct labeling of antibodies, proteins, peptides or other biomolecules (See protocol for further information) and allow conjugations to be set up in seconds, and used within minutes (less than 20 minutes).

Our Lightning-Link Rapid technology can be used to label small quantities of antibody with 100% recovery. The researcher simply pipettes the antibody or other biomolecule into a vial of lyophilized mixture containing the label of interest and incubates the reaction for only 15 minutes (for more details please watch the video below). As there are no desalting or dialysis steps that commonly interrupt traditional protein labeling procedures.

Features Benefits
Quick and easy to use Save time, no special knowledge required
No separation steps 100% recovery - no antibody/protein loss
Can be used in a wide range of applications Flexible
Freeze dried Ships at ambient temperature, long shelf-life
Fully scalable (10 ug to 1 g or more) Easy transfer from R&D to manufacturing
Stringently QC tested Consistent high quality, excellent batch-to-batch reproducibility
Large number of labels available Experimental flexibility
Reliable: nearly 300 references Successfully used in many fields of research


The attachment of biotin to biomolecules is an important laboratory technique. Biotin binds to the tetrameric avidin proteins, including streptavidin and neutravidin, with exceptionally high affinity, and this interaction is exploited in various applications such as western blotting, immunohisthochemistry and ELISA. Our biotinylation kits are optimised to create high quality conjugates and minimise assay background.

Lightning-Link Biotin Has been optimized for two separate applications:
  • Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used.
  • Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).


Learn more about Lightning-Link™ Conjugation Kits by reading FAQs

For more information please check out these useful links!
Antibody Labeling Guide
Antibody Conjugation Illustrated Assay
Kit Type
Antibody Labeling Kit

Applications/Dilutions

Application Notes
By circumventing the desalting or dialysis steps that commonly interrupt traditional antibody conjugation procedures, LightningLink technology can be used to label both small (e.g. 10 ug) and large quantities of primary antibodies with ease. Batch-to-batch variation upon scale up is minimal as the process is so simple, and recoveries are always 100%. This kit is supplied with 3 vials, each suitable for labeling up to 200 ug of antibody.
Publications
Read Publications using 370-0010.

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 1 or 3 glass vial(s) of Lightning-Link Rapid mix
  2. 1 vial of LL-Rapid Modifier reagent
  3. 1 vial of LL-Rapid Quencher reagent

Notes

This product is manufactured by Abcam and distributed by Novus Biologicals.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt and this statement overrides any mentioned guarantee period on the limitations section of this products datasheet. Please contact technical@novusbio.com with questions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Lightning-Link (R) Rapid Type A Biotin Kit (370-0010)(5)


Reviews for Lightning-Link (R) Rapid Type A Biotin Kit (370-0010) (0)

There are no reviews for Lightning-Link (R) Rapid Type A Biotin Kit (370-0010). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for Lightning-Link (R) Rapid Type A Biotin Kit (370-0010). (Showing 1 - 3 of 3 FAQs).

  1. Hi, I'm interested in buying your Lightning-Link Biotin labelling kit. I would like to couple biotin to an antibody, which would bind to an antigen in a sandwich assay format. Then streptavidin-coated nanoparticles would be used as the detection scheme. Which type is best for this purpose, type A or type B? Since Type A is for when Strep is used as a detection reagent, but Type B says its best for when Strep is immobilised to the suface of nanoparticles. In my case it is both.
    • Type B would be the preferred kit.
  2. Whats the difference between Lightning-Link (R) Rapid Type A and Type B for Biotin antibody labeling?
    • Type A - Intended for assays in which a streptavidin-labeled detection reagent will be used.Type B - Optimised for assays in which the biotinylated protein is captured by streptavidin immobilized on a surface (ie plates, nitrocellulose, magnetic beads etc).
  3. What about Biotin is different for Type A and Type B 370-0010 and 371-0010?
    • The actual biotin that is used in the label is the same biotin. Biotin A has been optimized particularly for performance; it should be used when the biotinylated antibody will undergo a wash step before coming into contact with streptavidin. For example in a sandwich ELISA, free label is not a problem because a wash step will remove unbound Biotin before you add streptavidin. Biotin B has been specially developed to further minimize excess left over biotin, and should be selected if the biotinylated antibody will NOT undergo a wash step before coming into contact with streptavidin. e.g. if the biotinylated antibody will be captured on streptavidin-beads. Free, left over biotin is a problem here because it is not washed away. It is captured by the beads and so affects the signal.

Additional Lightning-Link (R) Rapid Type A Biotin Products

Blogs on Lightning-Link (R) Rapid Type A Biotin

There are no specific blogs for Lightning-Link (R) Rapid Type A Biotin, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

  SDS - Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
  Manual - Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit
  PDF Datasheet
  Request CofA

Review this Product

Be the first to review our Lightning-Link (R) Rapid Type A Biotin Antibody Labeling Kit and receive a gift card or discount.