Human Kidney Whole Tissue Lysate (Adult Whole Tumor) Summary
| Description |
We recommend adding 1 x sample buffer with 5% BME (or other reducing agent) prior to use. |
| Specificity |
Total Protein - Human Tumor Tissue: Kidney |
Applications/Dilutions
| Dilutions |
|
| Application Notes |
Western Blot, Immunoprecipitation, Electrophoresis, Enzymatic activity analysis, Protein-protein interaction, Tissue specific expression |
Packaging, Storage & Formulations
| Storage |
Store at -80C. Avoid freeze-thaw cycles. |
| Buffer |
HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors |
| Concentration |
5.0 mg/ml |
Lysate Details for Array
| Type |
Tissue |
| Life Stage |
Adult |
| Tissue |
Digestive/Waste |
| Protein State |
Native |
| Tissue Condition |
Tumor |
| Subcellular Fraction |
Whole |
Background
Tissue total protein is prepared from whole tissue homogenates and presents a consistent pattern on SDS-PAGE analysis.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are
guaranteed for 6 months from date of receipt.
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Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
Video Protocols
FAQs for Kidney Lysate (NB820-59429). (Showing 1 - 3 of 3 FAQ).
-
What are the protease inhibitors? How much KCL, EDTA, magnesium chloride, sucrose, HEPES, glycerol is used?
- The information you have requested is proprietary. I am sorry for any inconvenience.
-
How is the protein concentration determined (BCA, nanodrop)?
- The protein concentration of this lysate is determined using the Bio-Rad DC Protein Assay Kit.
-
Is densitometry analysis performed to determine if the size of the bands of the different biomarkers tested in Western blot are the same across lots?
- No, we do not perform densitometry to quantitate the band intensity, as long as we see an obvious band, it will pass our QC.
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