SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. ...read more
Ready-to-use PVDF membrane with 11 mouse tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Mouse Tissues NBP2-30111 is a simple and fast solution for screening proteins from various mouse tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,...immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
Show More
Preparation Method
INSTA-Blot™ Mouse Tissues NBP2-30111contains denatured proteins from mouse lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blottedfor 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried andpackaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.
Applications/Dilutions
Dilutions
SDS-Page
Application Notes
Tissues are from Balb/c mice. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail(PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20%glycerol, 0.4% B-mercaptoethanol).
Mark12 is used as the molecular weight marker for this product.
Alternate Names for INSTA-Blot Mouse Tissues
INSTA Blot Mouse Tissues
INSTABlot Mouse Tissues
INSTA-Blot Mouse
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
Publications for INSTA-Blot Mouse Tissues (NBP2-30111)(3)
Reviews for INSTA-Blot Mouse Tissues (NBP2-30111) (0)
There are no reviews for INSTA-Blot Mouse Tissues (NBP2-30111).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
View specific protocols for INSTA-Blot Mouse Tissues (NBP2-30111):
![SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Mouse-Tissues-SDS-Page-NBP2-30111-img0001.jpg "SDS-Page: INSTA-Blot Mouse Tissues [NBP2-30111] - Mouse Tissues. Approximately 20 ug per lane of different mouse tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
