INSTA-Blot Human Tissues Summary
| Background |
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Tissues NBP2-30113 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,... immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop. |
Applications/Dilutions
| Dilutions |
- Western Blot reported in scientific literature (PMID 24704276)
|
| Application Notes |
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). |
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 24704276)
Packaging, Storage & Formulations
| Storage |
Store at room temperature. |
Alternate Names for INSTA-Blot Human Tissues
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are
guaranteed for 1 year from date of receipt.
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