Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and ...read more
Ready-to-use PVDF membrane with 11 human tissue lysates (50 ug total protein per lane).
Background
INSTA-Blot(R) is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot(R); Human Tissues NBP2-31378 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot(R);, immuno...blotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and detection.
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Preparation Method
INSTA-Blot͐ Human Tissues. NBP2-31378 contains denatured proteins from human lysates loaded at 50 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol.
Applications/Dilutions
Dilutions
Western Blot reported in scientific literature (PMID 25615412)
Application Notes
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended with 4x Laemmli sample buffer with B-mercaptoethanol and denatured in boiling water.
Publications
Read Publications using NBP2-31378 in the following applications:
Human reactivity reported in scientific literature (PMID:32839274).
Packaging, Storage & Formulations
Storage
Store at room temperature.
Notes
The molecular weight markers run on this blot are Mark12(TM) unstained standards: 200, 116.3, 97.4, 66.3, 55.4, 36.5, 21.5, 14.4, 6
Alternate Names for INSTA-Blot Human Tissues
INSTA Blot Human Tissues
INSTABlot Human Tissues
INSTA-Blot Human
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
Publications for INSTA-Blot Human Tissues (NBP2-31378)(4)
We have publications tested in 1 confirmed species: Human.
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Product General Protocols
View specific protocols for INSTA-Blot Human Tissues (NBP2-31378):
FAQs for INSTA-Blot Human Tissues (NBP2-31378). (Showing 1 - 1 of 1 FAQs).
I need to use the pancreas tissue as a positive control with my western blot in 12% acrylamide gel. Is it possible to have NBP2-30113 in high gel percentage 12% acrylamide?
We manufacture the blots using 4-20% gradient gels as denoted on the data sheet link. All of the lysates we use to generate the blot are available separately. By purchasing individual lysates you can run them on the gel type of your choice.
![Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Tissues-Western-Blot-NBP2-31378-img0001.jpg "Western Blot: INSTA-Blot Human Tissues [NBP2-31378] - SDS-Page: INSTA-Blot Human Tissues [NBP2-31378] - Approximately 50 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
