Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. ...read more
Ready-to-use PVDF membrane with 11 human tissue lysates (20 ug total protein per lane).
Background
INSTA-Blot͐ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot͐ Human Tissues NBP2-30113 is a simple and fast solution for screening proteins from various human tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With INSTA-Blot͐,... immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Preparation Method
INSTA-Blot͐ Human Tissues NBP2-30113 contains denatured proteins from human lysates loaded at 20 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution
by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot͐ is sold ready-to-use in your western blot protocol
Applications/Dilutions
Dilutions
Western Blot
Application Notes
Tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol). Use in western blot reported in scientific literature (PMID: 24704276) .
Publications
Read Publications using NBP2-30113 in the following applications:
Human reactivity reported in scientific literature (PMID: 24704276)
Packaging, Storage & Formulations
Storage
Store at room temperature.
Notes
Mark12 is used as the molecular weight marker for this product.
Alternate Names for INSTA-Blot Human Tissues
INSTA Blot Human Tissues
INSTABlot Human Tissues
INSTA-Blot Human
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.
Publications for INSTA-Blot Human Tissues (NBP2-30113)(16)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 2 applications: IF/IHC, WB.
Urban D. Characterization of VPS16B, a Novel Protein Involved in Platelet alpha-Granule Biogenesis Thesis. 2014-01-01 (WB)
Details: INSTA-Blot Multi-species Brain,Testis and Ovary used for WB analysis of VPS16B with GAPDH as a loading control. Highest VPS16B expression levels were seen in heart, skeletal muscles and brain, while small intestine, liver and testis also showed detectable levels of this protein (Figure 3.11) [Note:- This publication cites catalog# IMB-120 / NBP2-30115, however, in reality, the customer used IMB-103 / NBP2-30113 ].
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Product General Protocols
View specific protocols for INSTA-Blot Human Tissues (NBP2-30113):
FAQs for INSTA-Blot Human Tissues (NBP2-30113). (Showing 1 - 1 of 1 FAQs).
I need to use the pancreas tissue as a positive control with my western blot in 12% acrylamide gel. Is it possible to have NBP2-30113 in high gel percentage 12% acrylamide?
We manufacture the blots using 4-20% gradient gels as denoted on the data sheet link. All of the lysates we use to generate the blot are available separately. By purchasing individual lysates you can run them on the gel type of your choice.
![Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis](http://images.novusbio.com/fullsize/INSTA-Blot-Human-Tissues-SDS-Page-NBP2-30113-img0001.jpg "Western Blot: INSTA-Blot Human Tissues [NBP2-30113] - Human Tissues. Approximately 20 ug per lane of human tissue lysates are resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker (Mark12); 2. Brain; 3. Heart; 4. Small Intestine; 5. Kidney; 6. Liver; 7. Lung; 8. Skeletal Muscle; 9. Stomach; 10. Spleen; 11. Ovary; 12. Testis")
