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INSTA-Blot Human Brain

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SDS-Page: INSTA-Blot Human Brain [NBP2-30119] - Approximately 30 ug per lane of tissue lysate is resolved by SDS-PAGE, transfered onto PVDF membrane and stained with amido black. Lanes: 1. Molecular weight marker; 2-12 ...read more

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Summary
Applications WB

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INSTA-Blot Human Brain Summary

Description
Ready-to-use PVDF membrane with human brain lysate (30 ug total protein per lane).
Background
INSTA-Blot™ is a ready-to-use PVDF membrane containing denatured protein from cells or tissue lysates. The INSTA-Blot™ Human Brain NBP2-30119 is a simple and fast solution for screening proteins from various human, mouse and rat tissues. We've streamlined the western blotting procedure by eliminating the sample aquisition, preparation, SDS-PAGE and electro-blotting steps. With IN...STA-Blot™, immunoblotting is an easy six-step procedure: blocking, primary antibody incubation, wash, secondary antibody incubation, wash, and develop.
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Preparation
Method
The INSTA-Blot™ Human Brain NBP2-30119 contains denatured proteins from tissue lysates loaded at 30 ug (total protein, Bradford Assay) per lane on a 4-20% Tris-Glycine mini gel. After resolution by SDS-PAGE at 125V for 1 hour the proteins are electro-blotted for 2 hours at 25V onto a PVDF membrane. The membrane is stained with amido black for visualization of proteins, dried and packaged under anoxic conditions. The INSTA-Blot™ is provided ready-to-use in your western blot protocol.

Applications/Dilutions

Dilutions
  • Western Blot reported in scientific literature (PMID 26198933)
Application Notes
Human tissues are from donors with no known disease. Tissues are lysed in lysis buffer (10 mM Tris, pH 8.0, 130 mM NaCl, 1% Triton X-100, 10 mM NaF, 10 mM NaPi, 10 mM NaPPi) containing Protease Inhibitor Cocktail (PIC) and PMSF. Prior to SDS-PAGE the tissue lysate is resuspended 1:1 with 2X SDS sample buffer (120 mM Tris-Hcl [pH 6.8], 20 mM EDTA, 4% SDS, 0.06% Bromophenol Blue, 20% glycerol, 0.4% B-mercaptoethanol).
Publications
Read Publication using NBP2-30119.

Packaging, Storage & Formulations

Storage
Store at room temperature.

Alternate Names for INSTA-Blot Human Brain

  • INSTA Blot Human Brain
  • INSTABlot Human Brain

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Western Blot Membranes are guaranteed for 1 year from date of receipt.

Publications for INSTA-Blot Human Brain (NBP2-30119)(1)

We have publications tested in 1 application: WB.


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Product General Protocols

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