ImmPACT™ VIP HRP Substrate Summary
| Description |
Features:
2-4 fold greater sensitivity than original substrate kit
Optimized for IHC/ICC/ISH and blots
Permanent mounting
Ideal for single and multiple labeling
Working solutions stable for up to 14 days at 4C
One year expiry date
Supplied as 120 mL of diluent and stock solutions in dropper bottles |
Applications/Dilutions
| Dilutions |
- Enzyme-linked Immunosorbent Spot
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
- In-situ Hybridization
- Western Blot
|
| Application Notes |
This kit consists of 120 mL of diluent and concentrated stock solutions of ImmPACT(TM) VIP reagents in convenient dropper bottles. This substrate formulation can be used on nylon or PVDF membranes but is not recommended for use on nitrocellulose membranes. Working solutions are stable for up to 14 days at 4C. Vector(R) VIP and ImmPACT(TM) VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- 120 ml ImmPACT™ VIP Diluent
- 2.5 ml ImmPACT™ VIP Reagent 2
- 2.5 ml ImmPACT™ VIP Reagent 3
- 3 ml ImmPACT™ VIP Reagent 1
- 3 ml ImmPACT™ VIP Reagent 4
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
Vector(R) VIP and ImmPACT VIP HRP substrates both produce intense, violet (purple) reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector VIP and ImmPACT VIP chromogens also provide excellent color contrast in pigmented tissues such as melanoma or retina (1). With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector VIP and ImmPACT VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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