ImmPACT™ DAB HRP Substrate Summary
| Description |
Features:
2-4 fold greater sensitivity than original substrate kit
Optimized for IHC/ICC/ISH and blots
Heat Stable
Permanent mounting
Ideal for single and multiple labeling
Working solutions stable for up to 14 days at 4C
One year expiry date
Supplied as 120 ml of diluent and concentrated DAB stock solution
|
Applications/Dilutions
| Dilutions |
- Enzyme-linked Immunosorbent Spot
- Immunocytochemistry
- Immunohistochemistry
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
- In-situ Hybridization
- Western Blot
|
| Application Notes |
This product is an entirely new formula for a diaminobenzidine based peroxidase substrate. ImmPACT(TM) DAB produces a dark brown reaction product that is crisper and is 3-4 times more sensitive than the original Vector DAB substrate kit. It can be used for both manual and automated staining methods. Sections stained with DAB can also be viewed by darkfield and electron microscopy. Slides developed with DAB can be dehydrated, cleared, and permanently mounted. The ImmPACT(TM) DAB working solution is stable for at least five days at room temperature and at least two weeks when stored at 4C. This product consists of 120 ml of diluent and a convenient concentrated stock solution of highly purified DAB. This product can also be used on blots.. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- ImmPACT™ DAB Chromogen concentrate (3.6 mL)
- ImmPACT™ DAB Diluent (120 mL)
|
Notes
This product is manufactured by Vector Laboratories and distributed by Novus Biologicals.
Background
DAB (3,3-diaminobenzidine) HRP substrate produces a dark brown reaction product and can be used for both immunohistochemical and blotting applications. DAB chromogen is effective as a single label or as a second color for multiple antigen labeling. Because of its heat-resistance, DAB can be used in IHC/ ISH double labeling applications. With the aid of imaging systems and software, the spectral profile of DAB can be distinguished from our other proprietary enzyme substrates in applications where antigens are co-localized.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are
guaranteed for 6 months from date of receipt.
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