Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - TLR8 stimulation with R-848 measured by SEAP Expression 293T cells were transfected with pCMV/TLR8 plasmid and pNF-kB/SEAP plasmid using ...read more
Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - R-848 specifically activated the TLR7- or TLR8-depedent NF-kB/SEAP reporter cells in a dose dependent manner.
Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand Summary
Description
CAS#: 144875-48-9.
Purity
>98%, by HPLC.
Applications/Dilutions
Dilutions
Functional
Application Notes
Identity determined by MS, 1H-NMR and 13C-NMR. This product is useful for activation of mouse TLR7 and human TLR7 and TLR8. Stimulation of TLR8 has been achieved with 0.5-5 ug/mL. Use in functional assays reported in scientific literature (PMID 26134251).
Publications
Read Publications using NBP2-26231 in the following applications:
Human reactivity reported in scientific literature (PMID: 25532693)
Packaging, Storage & Formulations
Storage
Store at -20 °C.
Purity
>98%, by HPLC.
Reconstitution Instructions
Dissolve in DMSO at a concentration of 10 mg/ml. If the substance is not fully soluble, it can be heated up to 60C to ensure complete solubilization. Also soluble in dichloromethane, 100% or 5% methanol; poorly soluble in acetonitrile or ethyl acetate.
Alternate Names for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand
Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand is a low molecular weight synthetic molecule of the Imidazoquinoline family. It has potent immunostimulatory activity. It stimulates antibody secretion, cytokine production, protection from apoptosis and CD80 up-regulation. R-848 has been shown to activate only TLR7 and TLR8. This activation is MyD88 dependent and leads to the induction of the transcription factor NF-kB.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
Publications for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand (NBP2-26231)(8)
We have publications tested in 1 confirmed species: Human.
We have publications tested in 1 application: Func.
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FAQs for Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand (NBP2-26231). (Showing 1 - 1 of 1 FAQs).
Can you provide the HPLC conditions for determining the purity of Imidazoquinoline Resiquimod, R-848?
Thank you for inquiry about the Imidazoquinoline Resiquimod, R-848, Cat No NBP2-26231. Each batch of NBP2-26231 is endotoxin free and equal to or greater than 98% pure by HPLC. Identity is determined by Mass Spec, 1H and 13C NMR .
Additional Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand Products
![Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - TLR8 stimulation with R-848 measured by SEAP Expression 293T cells were transfected with pCMV/TLR8 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 2 ug/mL or 5 ug/mL of R-848 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorterAssay kit.](http://images.novusbio.com/fullsize/Imidazoquinoline-Resiquimod-R-848-TLR7-and-TLR8-ligand-NBP2-26231-img0004.jpg "Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - TLR8 stimulation with R-848 measured by SEAP Expression 293T cells were transfected with pCMV/TLR8 plasmid and pNF-kB/SEAP plasmid using Lipofectamin 2000. After 48 hrs of transfection, 2 ug/mL or 5 ug/mL of R-848 was added. Cells were incubated at 37C for 24 hrs. Transfected cell supernatant was collected and analyzed using NF-kB SEAPorterAssay kit.")
![Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - R-848 specifically activated the TLR7- or TLR8-depedent NF-kB/SEAP reporter cells in a dose dependent manner.](http://images.novusbio.com/fullsize/Imidazoquinoline-Resiquimod-R-848-TLR7-and-TLR8-ligand-NBP2-26231-img0005.jpg "Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - R-848 specifically activated the TLR7- or TLR8-depedent NF-kB/SEAP reporter cells in a dose dependent manner.")
![Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - Formula: C17H22N4O2](http://images.novusbio.com/fullsize/Imidazoquinoline-Resiquimod-R-848-TLR7-and-TLR8-ligand-NBP2-26231-img0007.jpg "Imidazoquinoline Resiquimod (R-848), TLR7 and TLR8 ligand [NBP2-26231] - Formula: C17H22N4O2")
