Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were ...read more
Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram ...read more
Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents ...read more
Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control ...read more
Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells ...read more
Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes ...read more
Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with ...read more
Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Affinity purified
Alternate Names for Mouse IgG2b Isotype Control (MPC-11)
IgG2B
Background
A mouse isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific mouse antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for the estimation of non-specific binding of target primary antibodies to cell surface antigens. IMGENEX offers a variety of mouse isotype controls including fluorescene conjugates and azide-free formats.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.
Publications for IgG2b Isotype Control (NBP2-27231)(4)
We have publications tested in 3 confirmed species: Human, Mouse, Rat.
We have publications tested in 3 applications: FLOW, Flow Cytometry, IF/IHC.
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![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0008.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [FITC] [NBP2-27229] - TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left), or TLR4-FITC (right). PPI negative, CD14+ cells were gated for analysis. Image using the FITC form of this antibody.")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0002.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Analysis of Alexa Fluor (R) 488 conjugate of NBP2-27231. Cell surface 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control. FITC-conjugated goat anti-mouse secondary")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control . FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0001.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Cell surface analysis of 10^6 human PBMC using 1 ug of mouse isotype control. The shaded histogram represents human PBMC alone; green represents mouse isotype control . FITC-conjugated goat anti-mouse secondary antibody was used for this experiment.")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0003.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - An intracellular stain was performed on Daudi cells with FoxO1/FKHR (83N7F8) antibody NBP2-31376AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.")
![Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cytometry-NBP2-27231-img0004.jpg "Flow Cytometry: Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 488] [NBP2-27231AF488] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.")
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody. Image using the Alexa Fluor 647 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0005.jpg "Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor (R) 647] [NBP2-27228] - Cell surface analysis of CD86 in human monocytes using 0.1 ug of NBP2-00308 conjugated to Alexa Fluor 647. The shaded histogram represents human monocytes alone; green represents mouse isotype control ; red represents CD86 this antibody. Image using the Alexa Fluor 647 form of this antibody.")
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0006.jpg "Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488. Image using the Alexa Fluor 700 form of this antibody.")
![Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral blood monocytes with EMMPRIN/CD147 (MEM-M6/2) antibody NB500-561APC (blue) and a matched isotype control NBP2-27231APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.](http://images.novusbio.com/fullsize/Mouse-Human-IgG2b-Isotype-Control-MPC-11-Flow-Cell-Surface-NBP2-27231-img0007.jpg "Flow (Cell Surface): Mouse, Human IgG2b Isotype Control (MPC-11) [NBP2-27231] - Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Allophycocyanin] [NBP2-27231APC] - A surface stain was performed on human peripheral blood monocytes with EMMPRIN/CD147 (MEM-M6/2) antibody NB500-561APC (blue) and a matched isotype control NBP2-27231APC (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.")