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Non-species specific IgG2a Isotype Control (IGG2a/6723)

Images

 
Formalin-fixed, paraffin-embedded human tonsil stained with Non-species specific IgG2a Isotype Control (IGG2a/6723). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, Flow, IHC, IP, ICC/IF
Clone
IGG2a/6723
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

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Non-species specific IgG2a Isotype Control (IGG2a/6723) Summary

Description
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Immunogen
Recombinant full-length human IgG2a protein
Localization
None
Specificity
To serve as a true negative control for various procedures, this rabbit monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT
  • Immunoprecipitation
  • Western Blot 1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin 1-2ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A purified

Alternate Names for Non-species specific IgG2a Isotype Control (IGG2a/6723)

  • IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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