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Rat IgG1 Isotype Control

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Flow, Func, IHC, ICC/IF
Clonality
Polyclonal
Host
Rat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Rat IgG1 Isotype Control Summary

Description
This Rat IgG1 immunoglobulin is useful as an isotype control for Rat IgG1 antibody.
Isotype
IgG1
Clonality
Polyclonal
Host
Rat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Flow (Cell Surface) 1:10 - 1:1000
  • Flow (Intracellular) 1:10 - 1:1000
  • Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • In vivo assay
Application Notes
In Vivo Assay usage was reported in scientific literature (PMID: 24591364). Use the isotype control at the same concentration as the experimental antibody. Optimal dilution of this antibody should be experimentally determined.
Publications
Read Publication using
DDXCR01 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris-NaCl, pH 8.
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for Rat IgG1 Isotype Control

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

Publications for IgG1 Isotype Control (DDXCR01)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: In Vivo.


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In Vivo
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Research Areas for IgG1 Isotype Control (DDXCR01)

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Blogs on IgG1.


  Read full blog post.

CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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