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Mouse IgG1 Isotype Control (MG1) [PE/Atto594]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, PAGE
Clone
MG1
Clonality
Monoclonal
Host
Mouse
Conjugate
PE/Atto594

Order Details

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Mouse IgG1 Isotype Control (MG1) [PE/Atto594] Summary

Specificity
Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. Fluorescein Conjugated Mouse IgG1 was proven by Dot Blot.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Selective Precipitation

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
  • SDS-Page
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Selective Precipitation

Alternate Names for Mouse IgG1 Isotype Control (MG1) [PE/Atto594]

  • IgG1
  • Igh-4
  • RGD1359539
  • VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG1 Isotype Control (NBP1-97005PEATT594) (0)

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Research Areas for IgG1 Isotype Control (NBP1-97005PEATT594)

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CD16 - Find me on macrophages, neutrophils and NK cells
CD16 is a lymphocyte Fc gamma type III low-affinity receptor for IgG and is represented by two similar genes, CD16A (Fc gamma RIII A) and CD16B (Fc gamma RIIIB). CD16A exists as a heterooligomeric polypeptide-anchored form in macrophages and NK cells....  Read full blog post.

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