![Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72672] - Western Blot of Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody [Alkaline Phosphatase].Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody [Alkaline Phosphatase] at 1:1,000 at 4C.Block for 30 min at RT.Reaction visualized using alkaline phosphatase substrate for 30 seconds.Predicted/Observed size: 55 kDa for Mouse IgG1.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-[Alkaline-Phosphatase]-Western-Blot-NBP1-72672-img0006.jpg "Western Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72672] - Western Blot of Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody [Alkaline Phosphatase].Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane.Primary antibody: none.Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary antibody [Alkaline Phosphatase] at 1:1,000 at 4C.Block for 30 min at RT.Reaction visualized using alkaline phosphatase substrate for 30 seconds.Predicted/Observed size: 55 kDa for Mouse IgG1.")
| Reactivity | MuSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Alkaline Phosphatase |
| Description | Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum |
| Immunogen | Mouse IgG1 heavy chain |
| Specificity | Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse heavy or light chain isotypes. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
| Dilutions |
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| Application Notes | This product has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
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![Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72672]](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgG1-Heavy-Chain-Secondary-Antibody-[Alkaline-Phosphatase]-Dot-Blot-NBP1-72672-img0005.jpg "Dot Blot: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase] [NBP1-72672]")
![Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for one hour at RT. Block for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.](http://images.novusbio.com/fullsize/nbp1-72672_rabbit-polyclonal-rabbit-anti-mouse-igg1-heavy-chain-secondary-antibody-alkaline-phosphatase-23520231752221.jpg "Dot Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Antigen: Mouse IgG1. Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4ng Lane 5 - 2.5ng. Primary antibody: none. Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 for one hour at RT. Block for 60 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds at RT.")
![Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 o/n at 4C. Block for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.](http://images.novusbio.com/fullsize/nbp1-72672_rabbit-polyclonal-rabbit-anti-mouse-igg1-heavy-chain-secondary-antibody-alkaline-phosphatase-2552023131314.jpg "Western Blot of Mouse IgG1 Secondary Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG1. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody [Alkaline Phosphatase]at 1:1,000 o/n at 4C. Block for 30 min at RT. Reaction visualized using alkaline phosphatase substrate for 30 seconds. Predicted/Observed size: 55 kDa for Mouse IgG1.")