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Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP]

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Goat anti-Mouse IgG1 Heavy Chain Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG1
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000-1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200-1:2000
  • Immunohistochemistry 1:200-1:2000
  • Western Blot 1:2000-1:20000
Readout System
Publications
Read Publications using
NB7511 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS containing 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG1 Antibody (NB7511)(19)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: B/N, ELISA, ICC/IF, WB.


Filter By Application
B/N
(1)
ELISA
(1)
ICC/IF
(1)
WB
(2)
All Applications
Filter By Species
Mouse
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NB7511 Applications Species
Mut M, Adiguzel Z, Cakir-Aktas C et al. Extracellular-Vesicle-Based Cancer Panels Diagnose Glioblastomas with High Sensitivity and Specificity Cancers 2023-07-26 [PMID: 37568598] (WB) WB
Wong TY, Lee KS, Russ BP Et al. Intranasal administration of BReC-CoV-2 COVID-19 vaccine protects K18-hACE2 mice against lethal SARS-CoV-2 challenge NPJ Vaccines 2022-03-15 [PMID: 35288576] (ELISA)

Details:
Citation using the HRP version of this antibody.
ELISA
Colli LM, Jessop L, Myers TA Et al. Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus American journal of human genetics 2021-08-09 [PMID: 34390653] (B/N) B/N
Rodriguez-Morales O, Cabrera-Mata JJ, Carrillo-Sanchez SDC et al Electrolyzed Oxidizing Water Modulates the Immune Response in BALB/c Mice Experimentally Infected with Trypanosoma cruzi Pathogens 2020-11-26 [PMID: 33238401] (ICC/IF, Mouse)

Details:
Citation using the HRP version of this antibody.
ICC/IF Mouse
Bigot P, Colli LM, Machiela MJ et al. Functional characterization of the 12p12 1 renal cancer-susceptibility locus implicates BHLHE41. Nat Commun. [PMID: 27384883] (WB)

Details:
Citation using the HRP form of this antibody.
WB
Lanckacker EA, Tournoy KG, Hammad H et al. Short cigarette smoke exposure facilitates sensitization and asthma development in mice. Eur Respir J. [PMID: 22903968]

Details:
Citation using the HRP form of this antibody.
Golden JW, Josleyn M, Mucker EM et al. Side-by-side comparison of gene-based smallpox vaccine with MVA in nonhuman primates. PLoS One. 2012-01-01 [PMID: 22860117]

Details:
Citation using the HRP form of this antibody.
Tawde SA, Chablani L, Akalkotkar A et al. Formulation and evaluation of oral microparticulate ovarian cancer vaccines. Vaccine. [PMID: 22750042]

Details:
Citation using the HRP form of this antibody.
Eisenbarth SC, Williams A, Colegio OR et al. NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Nature. [PMID: 22538615]

Details:
Citation using the HRP form of this antibody.
Yu L, Yamagishi J, Zhang S et al. Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice. Parasitol Int . [PMID: 22537971]

Details:
Citation using the HRP form of this antibody.
Show All 19 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG1 Antibody (NB7511). (Showing 1 - 1 of 1 FAQs).

  1. According to the Product sheet, binding specificity of Ab(#NB7511) is Mouse IgG1. Would the antibody cross-react with other IgG subclasses?
    • By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG1. Cross reactivity to mouse IgA, IgM, IgG2a, IgG2b, IgG2c, IgG3 and IgE is undetectable. Some hybridoma clones may express aberrant immunoglobulin-related peptides that are improperly recognized by this antibody.

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