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Rabbit IgG Isotype Control [Alkaline Phosphatase]

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SDS-Page: Rabbit IgG Isotype Control [Alkaline Phosphatase] [NBP1-97162] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Alkaline Phosphatase Conjugated. Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment. Lane 4: Reduced Rabbit IgM Whole Molecule. Load: 1 ug for F(ab) and F(c); 1.2 ug for IgG and IgM.
SDS-Page: Rabbit IgG Isotype Control [Alkaline Phosphatase] [NBP1-97162] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Alkaline Phosphatase Conjugated. Lane 2: Reduced Rabbit IgG F(ab) ...read more

Product Details

Summary
Reactivity RbSpecies Glossary
Applications PAGE
Host
Rabbit
Conjugate
Alkaline Phosphatase

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Agarose
NBP1-97118
Alexa Fluor 405
NBP2-36463AF405
Alexa Fluor 488
NBP2-36463AF488
Alexa Fluor 647
NBP2-36463AF647
Alexa Fluor 700
NBP2-36463AF700
Biotin
NBP1-96846
Biotin
NBP2-36463B
DyLight 350
NBP2-36463UV
DyLight 405
NBP2-36463V
DyLight 488
NBP2-36463G
DyLight 550
NBP2-36463R
DyLight 650
NBP2-36463C
DyLight 680
NBP2-36463FR
DyLight 755
NBP2-36463IR
FITC
NBP1-96877
HRP
NBP1-96797
HRP
NBP2-36463H
PE/Cy7
NBP2-36463PECY7
Rhodamine
NBP1-96878
Texas Red
NBP1-96845
Unconjugated
NBP2-36463
mFluor Violet 450 SE
NBP2-36463MFV450
mFluor Violet 500 SE
NBP2-36463MFV500
mFluor Violet 610 SE
NBP2-36463MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP2-36463
Purified
NBP2-36463
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Rabbit IgG Isotype Control [Alkaline Phosphatase] Summary

Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by conjugation and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG, anti-Rabbit Serum and anti-Alkaline Phosphatase (calf intestine).
Isotype
IgG
Host
Rabbit
Gene
IGHG1
Purity
Multi-step

Applications/Dilutions

Dilutions
  • SDS-Page 1:100 - 1:2000
Application Notes
This product suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Multi-step

Alternate Names for Rabbit IgG Isotype Control [Alkaline Phosphatase]

  • IgG
  • Immunoglobulin G
  • ImmunoglobulinG

Background

IgG is a monomeric immunoglobulin, built of two heavy chains gamma and two light chains. Each molecule has two antigen binding sites. This is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. It can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by complement activation (classic pathway), opsonization for phagocytosis and neutralisation of their toxins. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGHG1