I want to use the rabbit IgG Isotype Ctrl (NB810-56910). But the datasheet says that the concentration is not relevant for this product. But how can I use the Isotype Control without knowing the concentration? For FACS the Isotype Ctrl need to be used at exactly the same concentration as the Test antibody. So could you please tell me the concentration of #NB810-56910. I want to use it as Ctrl for #NBP1-49416.

Name: Rabbit IgG Isotype Control
Brand: Novus Biologicals
SKU: NB810-56910
Rating: 5 (6 reviews)

Question

Accepted Answer

The concentration of NB810-56910 is 5 mg/ml.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	RbSpecies Glossary
Applications	WB, Flow, ICC/IF, IHC, IP, ChIP
Clonality	Polyclonal
Host	Rabbit
Conjugate	Unconjugated
Concentration	5 mg/ml

Description	This Rabbit IgG immunoglobulin is useful as an isotype control for Rabbit IgG antibody.
Specificity	By immunoelectrophoresis the IgG was shown to 1) react with antiserum specific for rabbit IgG, 2) not react with antiserum specific for IgA or IgM, and 3) produce a single precipitin arc with antiserum against rabbit serum identical to that produced with anti-IgG antisera.
Isotype	IgG
Clonality	Polyclonal
Host	Rabbit
Gene	IGHG1
Purity	Ion exchange chromatography

Storage	Store at 4C.
Buffer	PBS.
Preservative	0.09% Sodium Azide
Concentration	5 mg/ml
Purity	Ion exchange chromatography

Publications using NB810-56910	Applications	Species
Marta Zaninello, Tim Schlegel, Hendrik Nolte, Mujeeb Pirzada, Elisa Savino, Esther Barth, Ines Klein, Hauke Wüstenberg, Tesmin Uddin, Lisa Wolff, Brunhilde Wirth, Helmar C. Lehmann, Jean-Michel Cioni, Thomas Langer, Elena I. Rugarli CLUH maintains functional mitochondria and translation in motoneuronal axons and prevents peripheral neuropathy Science Advances 2024-05-31 [PMID: 38809982]
Anni Suominen, Guillem Saldo Rubio, Saku Ruohonen, Zoltán Szabó, Lotta Pohjolainen, Bishwa Ghimire, Suvi T Ruohonen, Karla Saukkonen, Jani Ijas, Sini Skarp, Leena Kaikkonen, Minying Cai, Sharon L Wardlaw, Heikki Ruskoaho, Virpi Talman, Eriika Savontaus, Risto Kerkelä, Petteri Rinne alpha -Melanocyte-stimulating hormone alleviates pathological cardiac remodeling via melanocortin 5 receptor. EMBO reports 2024-04-15 [PMID: 38454158]
Thapa K, Kadiri JJ, Saukkonen K et al. Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver eLife 2023-07-25 [PMID: 37490042]
Cinato M, Mardani I, Miljanovic A et al. Cardiac Plin5 interacts with SERCA2 and promotes calcium handling and cardiomyocyte contractility Life Science Alliance 2023-04-01 [PMID: 36717246]
Porman AM, Roberts JT, Duncan ED et al. A single N6-methyladenosine site regulates lncRNA HOTAIR function in breast cancer cells PLoS biology 2022-11-28 [PMID: 36441764] (IP)	IP
Porman A, Roberts J, Chrupcala M et al. A single N6-methyladenosine site in lncRNA HOTAIR regulates its function in breast cancer cells bioRxiv 2020-06-09 [PMID: 36441764] (IP)	IP
JankovicovA J, SecovA P, Horovska L et al. Distribution of tetraspanins in bovine ovarian tissue and fresh/vitrified oocytes Histochemistry and cell biology 2022-10-15 [PMID: 36242635]
McKernan CM, Khatri A, Hannigan M et al. ABL kinases regulate translation in HER2+ cells through Y-box-binding protein 1 to facilitate colonization of the brain Cell reports 2022-08-30 [PMID: 36044842] (IP, Rabbit)	IP	Rabbit
Le NPK, do Nascimento AF, Schneberger D et al. Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model Respiratory research 2022-06-22 [PMID: 35733161] (IF/IHC)	IF/IHC
AntalikovA J, SecovA P, MichalkovA K et al. Expression of alpha V integrin and its potential partners in bull reproductive tissues, germ cells and spermatozoa International journal of biological macromolecules 2022-04-09 [PMID: 35413326] (IF/IHC, ICC/IF)	IF/IHC, ICC/IF
Xie L, Dong P, Qi Y et al. BRD2 compartmentalizes the accessible genome Nature Genetics 2022-04-11 [PMID: 35410381] (Immunoprecipitation)	Immunoprecipitation
Jorgensen M, Joseph P, Posgai Al Et Al. ACE2 chromogenic immunostaining protocol optimized for formalin-fixed paraffin-embedded Human tissue sections STAR protocols 2021-09-17 [PMID: 34308375]
Yu X, Chen X, Amrute-Nayak M et al. MARK4 controls ischaemic heart failure through microtubule detyrosination Nature 2021-05-26 [PMID: 34040253] (IF/IHC) Details: Immunohistochemistry was performed using the Rabbit IgG antibody on sections of mouse hearts. The fluorescent images provided evidence that MARK4 deficiency preserves cardiac function after myocardial infarction.	IF/IHC
Shakya B, Patel SD, Tani Y, Egan ES. Erythrocyte CD55 mediates the internalization of Plasmodium falciparum parasites eLife 2021-05-24 [PMID: 34028351]
Roberts JT, Porman AM, Johnson AM Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline RNA (New York, N.Y.) 2021-04-01 [PMID: 33376190] (IP)	IP
Qazi IH, Yang H, Wei S et al. Dietary selenium deficiency and supplementation differentially modulate the expression of two ER-resident selenoproteins (selenoprotein K and selenoprotein M) in the ovaries of aged mice: Preliminary data Reprod Biol 2020-07-28 [PMID: 32736983] (IF/IHC)	IF/IHC
Ho JJD, Balukoff NC, Theodoridis PR et al. A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism Nat Commun 2020-05-29 [PMID: 32472050] (IP) Details: Citation using the HRP format of this antibody.	IP
Bengts S, Shamoun L, Kunath A et al. Altered IL-32 Signaling in Abdominal Aortic Aneurysm J. Vasc. Res. 2020-05-20 [PMID: 32434199] (IF/IHC)	IF/IHC
Antalikova J, Secova P, Horovska L et al. Missing Information from the Estrogen Receptor Puzzle: Where Are They Localized in Bull Reproductive Tissues and Spermatozoa? Cells 2020-01-10 [PMID: 31936899] (ICC/IF, Bovine)	ICC/IF	Bovine
Muschter D, Fleischhauer L, Taheri S et al. Sensory neuropeptides are required for bone and cartilage homeostasis in a murine destabilization-induced osteoarthritis model Bone 2020-01-08 [PMID: 31926346] (IHC-P, Mouse)	IHC-P	Mouse
Czarnek M, Bereta J Proteolytic Processing of Neuregulin 2 Mol. Neurobiol. 2019-12-14 [PMID: 31838721] (IF/IHC)	IF/IHC
Das B, Pal B, Bhuyan R et al. MYC Regulates the HIF2 alpha Stemness Pathway via Nanog and Sox2 to Maintain Self-Renewal in Cancer Stem Cells versus Non-Stem Cancer Cells Cancer Res. 2019-07-02 [PMID: 31266772] (Chemotaxis, Human, Mouse)	Chemotaxis	Human, Mouse
Cansby E, Magnusson E, Nunez-Duran E et al. STK25 Regulates Cardiovascular Disease Progression in a Mouse Model of Hypercholesterolemia Arterioscler. Thromb. Vasc. Biol. 2018-06-21 [PMID: 29930001] (Rabbit)		Rabbit
Iida Y, Ciechanover A, Marzese DM et al. Epigenetic Regulation of KPC1 Ubiquitin Ligase Effects the NF-kB Pathway in Melanoma. Clin. Cancer Res. 2017-04-07 [PMID: 28389511]
Molina-Serrano D, Schiza V, Demosthenous C et al. Loss of Nat4 and its associated histone H4 N-terminal acetylation mediates calorie restriction-induced longevity EMBO reports 2016-12-01 [PMID: 27799288]
Zabkiewicz J, Gilmour M, Hills R et al. The targeted histone deacetylase inhibitor tefinostat (CHR-2845) shows selective in vitro efficacy in monocytoid-lineage leukaemias. Oncotarget 2016-03-29 [PMID: 26934551] (FLOW)	FLOW
Bisoendial R, Tabet F et al. Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis. Arterioscler Thromb Vasc Biol 2015-01-11 [PMID: 26359513] (IF/IHC, Mouse)	IF/IHC	Mouse
Bergstrom B, Aune MH, Awuh JA et al. TLR8 Senses Staphylococcus aureus RNA in Human Primary Monocytes and Macrophages and Induces IFN-B Production via a TAK1-IKKB-IRF5 Signaling Pathway J. Immunol. 2015-06-17 [PMID: 26085680] (ICC/IF, Human)	ICC/IF	Human
Xiang L, Gilkes DM, Hu H et al. HIF-1alpha and TAZ serve as reciprocal co-activators in human breast cancer cells. Oncotarget. 2015-05-20 [PMID: 26059435] (IP, Chemotaxis, Human)	IP, Chemotaxis	Human
Xiang L, Gilkes DM, Hu H et al. Hypoxia-inducible factor 1 mediates TAZ expression and nuclear localization to induce the breast cancer stem cell phenotype. Oncotarget. 2014-01-01 [PMID: 25587023] (Chemotaxis, IP)	Chemotaxis, IP
Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014-12-05 [PMID: 25485521] (Chemotaxis) Details: p62/SQSTM1 antibody used for WB on RAW264.7 cells subjected to RANKL and M-CSF mediated osteoclast differentiation as well as hypoxic stress (Fig. 1E )	Chemotaxis
Hu H, Takano N, Xiang L et al. Hypoxia-inducible factors enhance glutamate signaling in cancer cells. Oncotarget 2014-10-15 [PMID: 25326682] (Chemotaxis)	Chemotaxis
Rozman S, Yousefi S, Oberson K et al. The generation of neutrophils in the bone marrow is controlled by autophagy. Cell Death Differ. 2014-10-17 [PMID: 25323583] (FLOW, Mouse)	FLOW	Mouse
Ksander BR, Kolovou PE, Wilson BJ et al. ABCB5 is a limbal stem cell gene required for corneal development and repair. Nature 2014-07-17 [PMID: 25030174] (WB)	WB
Lavoie JR, Ormiston ML, Perez-Iratxeta C et al. Proteomic analysis implicates translationally controlled tumor protein as a novel mediator of occlusive vascular remodeling in pulmonary arterial hypertension. Circulation. 2014-05-27 [PMID: 24657995]
Perr J, Langen A, Almahayni K et al. RNA binding proteins and glycoRNAs form domains on the cell surface for cell penetrating peptide entry bioRxiv 2023-09-05 (FLOW)	FLOW
Maynard KM, Arvindam U, Cross M, Firpo MT. Potentially immunogenic proteins expressed similarly in human embryonic stem cells and induced pluripotent stem cells. Exp Biol Med (Maywood). 2014-01-22 [PMID: 24595983] (FLOW)	FLOW
Ma XH, Piao SF, Dey S et al. Targeting ER stress-induced autophagy overcomes BRAF inhibitor resistance in melanoma. J Allergy Clin Immunol. [PMID: 24569374] (WB, PLA, Human) Details: Citation using the Agarose Immobilized form of this antibody.	WB, PLA	Human
Stratmann AT, Fecher D, Wangorsch G et al. Establishment of a human 3D lung cancer model based on a biological tissue matrix combined with a Boolean in silico model. Mol Oncol 2014-03-01 [PMID: 24388494]
Gilkes DM, Xiang L, Lee SJ et al. Hypoxia-inducible factors mediate coordinated RhoA-ROCK1 expression and signaling in breast cancer cells. Proc Natl Acad Sci U S A 2014-01-21 [PMID: 24324133] (Chemotaxis, Human)	Chemotaxis	Human
Cai Z, Luo W, Zhan H, Semenza GL. Hypoxia-inducible factor 1 is required for remote ischemic preconditioning of the heart. Proc Natl Acad Sci U S A. 2013-10-22 [PMID: 24101519] (Chemotaxis, Rabbit)	Chemotaxis	Rabbit
Mazzoni EO, Mahony S, Closser M et al. Synergistic binding of transcription factors to cell-specific enhancers programs motor neuron identity. Nat Neurosci 2013-07-21 [PMID: 23872598] (IP, Mouse)	IP	Mouse
Pallet N, Sirois I, Bell Cet al. A comprehensive characterization of membrane vesicles released by autophagic human endothelial cells. Proteomics 2013-02-22 [PMID: 23436686] (FLOW)	FLOW
Durrani SR, Montville DJ, Pratt AS et al. Innate immune responses to rhinovirus are reduced by the high-affinity IgE receptor in allergic asthmatic children. J Allergy Clin Immunol 2012-08-01 [PMID: 22766097]
Estronca LM, Silva JC, Sampaio Jlet al. Molecular Etiology of Atherogenesis - In Vitro Induction of Lipidosis in Macrophages with a New LDL Model. PLoS One;7(4). 2012-01-01 [PMID: 22514671] (Mouse)		Mouse
Yeung PL, Denissova NG, Nasello C, Hakhverdyan Z, Chen JD, Brenneman MA. Promyelocytic leukemia nuclear bodies support a late step in DNA double-strand break repair by homologous recombination. J Cell Biochem;113(5):1787-99. 2012-05-01 [PMID: 22213200] (ICC/IF, Rabbit)	ICC/IF	Rabbit
Lambotin M, Baumert TF, Barth H et al. Distinct intracellular trafficking of hepatitis C virus in myeloid and plasmacytoid dendritic cells. J Virol 2010-09-01 [PMID: 20573834]
Gill MA, Bajwa G, George TA et al. Counterregulation between the FcepsilonRI pathway and antiviral responses in human plasmacytoid dendritic cells. J Immunol 2010-06-01 [PMID: 20410486]
Han Z, Verma D, Hilscher C et al. General and target-specific RNA binding properties of Epstein-Barr virus SM posttranscriptional regulatory protein. J Virol 2009-11-01 [PMID: 19726500]
Schiller, N et al. Participation of macrophages in atherosclerotic lesion morphology in LDLr-/- mice. Journal of Lipid Research. [PMID: 15175354] Details: Citation using the Agarose Immobilized form of this antibody.
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Rabbit IgG Isotype Control

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	A G 05/26/2017
Application:
Species:

	Verified Customer 09/29/2015
Application:	Flow
Species:	Mouse

	Verified Customer 08/29/2015
Application:	IHC-Fr
Species:	Mouse