![Dot Blot: Rabbit anti-Human IgG Heavy Chain Secondary Antibody [NBP2-22138] - Dot Blot of Rabbit anti-Human IgG Secondary Antibody [Alkaline Phosphatase]. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.](http://images.novusbio.com/fullsize/Rabbit-anti-Human-IgG-gamma-chain-Secondary-Antibody-Unconjugated-Pre-adsorbed-Dot-Blot-NBP2-22138-img0001.jpg "Dot Blot: Rabbit anti-Human IgG Heavy Chain Secondary Antibody [NBP2-22138] - Dot Blot of Rabbit anti-Human IgG Secondary Antibody [Alkaline Phosphatase]. Antigen: Human IgG. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng. Incubated for 1hr at RT; blocked for 60 min at RT. Image using the Alkaline Phosphatase form of this antibody.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, DB, ELISA, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Description | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. |
| Immunogen | Human IgG gamma heavy chain |
| Specificity | No reaction was observed against Human IgM or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Multi-step |
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| Application Notes | This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Preservative | 0.01% Sodium Azide |
| Purity | Multi-step |
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