Affinity purified antibody is > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. Fluorescein-5-Isothiocyanate (FITC) Ex = 494 nm; Em = 518 nm
Immunogen
This Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] was developed against purified canine IgG (H&L).
Specificity
Based on IEP, this Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] reacts with heavy gamma chains on dog IgG and light chains on all dog immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified
Applications/Dilutions
Dilutions
ELISA 1:2000 - 1:20000
Immunoassay
Immunocytochemistry/ Immunofluorescence
Immunofluorescence
Immunohistochemistry 1:500 - 1:5000
Immunohistochemistry-Paraffin
Western Blot 1:2000 - 1:20000
Application Notes
This conjugate is suitable for all immunoassay applications.
Reviewed Applications
Read 1 Review rated 4 using NBP1-75720 in the following applications:
Based on IEP, no reactivity is observed to non-immunoglobulin canine serum immunoglobulins
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
Affinity purified
Background
Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IgG (H+L) Antibody (NBP1-75720) (0)
There are no publications for IgG (H+L) Antibody (NBP1-75720).
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![Immunocytochemistry/Immunofluorescence: Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] [NBP1-75720] - Rat liver stained with anti-nuclear antibody followed by Goat anti-Canine IgG (H+L) Secondary Antibody [FITC]. Image from verified customer review.](http://images.novusbio.com/fullsize/Goat-anti-Canine-IgG-H+L-Secondary-Antibody-FITC-Immunocytochemistry-Immunofluorescence-NBP1-75720-img0002.jpg "Immunocytochemistry/Immunofluorescence: Goat anti-Canine IgG (H+L) Secondary Antibody [FITC] [NBP1-75720] - Rat liver stained with anti-nuclear antibody followed by Goat anti-Canine IgG (H+L) Secondary Antibody [FITC]. Image from verified customer review.")
