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Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [PE]

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Product Details

Summary
Reactivity GpSpecies Glossary
Applications WB, ELISA, Flow, Func, ICC/IF, IHC, IP, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
PE

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Goat anti-Guinea Pig IgG (H+L) Secondary Antibody [PE] Summary

Immunogen
This Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against purified guinea pig IgG (H&L).
Specificity
Based on IEP, this Goat anti-Guinea Pig IgG (H+L) Secondary Antibody (Pre-adsorbed) heavy gamma chains on guinea pig IgG and light chains on all guinea pig immunoglobulins. This antibody has been pre-adsorbed against bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat or sheep
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunoassay
  • Immunocytochemistry
  • Immunocytochemistry/ Immunofluorescence
  • Immunodiffusion
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin guinea pig serum immunoglobulins and IgG from bovine, chicken, goat, hamster, horse, human, mouse, rabbit, rat, or sheep

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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