Human cell line stained with humanized primary antibody and detected with NB7488 Goat anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed). Image from verified customer review. Image using the FITC format of this ...read more
Antiserum was cross adsorbed using a monkey immunosorbent to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Immunogen
This Goat anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against human IgG-heavy and light chain.
Specificity
By immunoelectrophoresis and ELISA this Goat anti-Human IgG (H+L) Secondary Antibody (Pre-adsorbed) reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Antiserum was cross adsorbed using a rhesus and cynomolgus monkey immunosorbent to remove cross reactive Antibodies. Less than 0.1% cross reactivity to monkey IgG was detected. This antibody may cross react with IgG from other species.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
Applications/Dilutions
Dilutions
ELISA 1:1000-1:20000; for coating plates 1:50-1:250
Antiserum was cross adsorbed using a monkey immunosorbent to remove cross reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Background
Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).
The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).
3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268
4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
Publications for IgG (H+L) Antibody (NB7487)(8)
We have publications tested in 2 applications: ELISA, WB.
FAQs for IgG (H+L) Antibody (NB7487). (Showing 1 - 4 of 4 FAQs).
Is this Ab specific for human IgG and doesn't cross react with non-human primates, rat and mouse?
NB7487 is cross-absorbed against non-human primate IgG but not rat and mouse. Unfortunately, since so much antibody is lost when cross-absorbing against non-human primate, it is not possible to cross-absorb against monkey, mouse and rat. We can only offer antibodies cross-absorbed against mouse and rat or against non-human primate but not all three. I apologize for the inconvenience.
Is NB7487 cross absorbed against mouse IgG?
For our Goat anti-Human IgG Secondary Antibody (NB7487), we have unfortunately not cross-absorbed this product against mouse IgG. This secondary has only been cross-absorbed using a monkey immunosorbent.
What is the precise buffer condition? The researcher to setup MSD assay told that some of the reagents need to be avoided such as glycerol; glycine; imidazole etc. I can see your webpage says the solution only contains PBS And sodium azide, which is good, but please let me know if the solution has additional additives.
There are no additional additives. This secondary antibody is formulated in PBS containing 0.09% Sodium Azide.
Analytical result of low cross-reactivity with monkey serum: What was your method of analysis? I know that you told there may be cross-reactivity with other serums; but have you tested this antibody with rat serum?
Antiserum was cross-adsorbed using a monkey immunosorbent to remove cross-reactive antibodies. The antibody to human IgG was isolated by affinity chromatography using antigen coupled to agarose beads. By immunoelectrophoresis and ELISA, this antibody reacts specifically with human IgG and with light chains common to other human immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to monkey IgG was detected. This antibody may cross react with IgG from other species but has not been explicitly tested (including rat serum).
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