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Canine IgG Fc Isotype Control [Biotin]

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Product Details

Summary
Reactivity CaSpecies Glossary
Applications ELISA, ICC/IF
Host
Canine
Conjugate
Biotin

Order Details

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Canine IgG Fc Isotype Control [Biotin] Summary

Description
For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room

This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Dog IgG, anti-Dog IgG F(c) and anti-Dog Serum
Specificity
No reaction was observed against anti-Dog IgG F(ab')2 or anti-Papain.
Isotype
IgG
Host
Canine
Purity
Multi-step

Applications/Dilutions

Dilutions
  • Fluorophore-linked immunosorbent assay
  • Immunocytochemistry/ Immunofluorescence
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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