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Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed)

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Format
Pre-adsorbed
Concentration
1.5 mg/ml

Order Details

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Goat anti-Human IgG Fc Secondary Antibody [Biotin] (Pre-adsorbed) Summary

Description
Purity > 95% based on SDS-PAGE. Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
Immunogen
Purified Human IgG, Fc fragment
Specificity
Based on IEP, this antibody reacts with heavy gamma chains on human IgG. This antibody has been pre-adsorbed against bovine, horse, mouse, and rabbit serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:2000 - 1:20000
  • Immunohistochemistry 1:500 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000 - 1:20000
Application Notes
This conjugate is suitable for all immunoassay applications.

Reactivity Notes

Based on IEP, no reactivity is observed to non-immunoglobulin human serum immunoglobulins, light chains on all human immunoglobulins, human IgG F(ab)'2 fragment, and serum proteins from bovine, horse, mouse, or rabbit

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
Preservative
0.05% Sodium Azide
Concentration
1.5 mg/ml
Purity
Affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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