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Rabbit anti-Goat IgG Fc Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed)

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 549
Format
Pre-adsorbed

Order Details

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Catalog# & Conjugate Size Price
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Allophycocyanin/Cy7
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Biotin
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DyLight 350
NBP1-74860UV
DyLight 405
NBP1-74860V
DyLight 488
NBP1-74860G
DyLight 550
NBP1-74860R
DyLight 594
NBP1-74860DL594
DyLight 650
NBP1-74860C
DyLight 680
NBP1-74860FR
DyLight 755
NBP1-74860IR
HRP
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Janelia Fluor 646
NBP1-74860JF646
PE
NBP1-74860PE
PE/Atto594
NBP1-74860PEATT594
PE/Cy5.5
NBP1-74860PECY55
PE/Cy7
NBP1-74860PECY7
PerCP
NBP1-74860PCP
Unconjugated
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View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-74860
Pre-adsorbed
NBP1-74860
Pre-adsorbed
NBP1-74861
Pre-adsorbed
NBP1-74862
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NBP1-74860-0.5mg
Pre-adsorbed
NBP1-74860JF646
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Rabbit anti-Goat IgG Fc Secondary Antibody [Janelia Fluor 549] (Pre-adsorbed) Summary

Immunogen
Purified Goat IgG Fc fragment
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on goat IgG. Cross Adsorbed: This antibody has been pre-adsorbed against human serum proteins to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with human IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on goat IgG.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Affinity purified

Notes



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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