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Goat anti-Mouse IgG Fc Secondary Antibody [HRP]

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Western Blot: Goat anti-Mouse IgG Fc fragment Secondary Antibody [HRP] [NB7561] - Analysis of Cadherin in Huh7 cell lysate. Image from verified customer review.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Goat anti-Mouse IgG Fc Secondary Antibody [HRP] Summary

Immunogen
Mouse IgG-Fc
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG. This may cross react with IgG from other species
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:5000
  • Immunohistochemistry 1:200 - 1:5000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:5000 - 1:50000
Application Notes
Chemiluminescent - use at a dilution of 1:1,000 - 1:30,000.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NB7561 in the following applications:

Publications
Read Publications using NB7561.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG Fc fragment Antibody (NB7561)(13)

We have publications tested in 1 application: WB.


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WB
(1)
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Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB7561 Applications Species
Jong Seung Lim, Eunkyoung Kim, Jin-Sook Song, Sunjoo Ahn Energy‑stress‑mediated activation of AMPK sensitizes MPS1 kinase inhibition in triple‑negative breast cancer. Oncology reports 2024-06-21 [PMID: 38904203]
Dégardin M, Gaudreault J, Oliverio R et al. Grafting Strategies of Oxidation-Prone Coiled-Coil Peptides for Protein Capture in Bioassays: Impact of Orientation and the Oxidation State ACS Omega 2023-07-28 [PMID: 37576632] (WB) WB
Jiang C, Zhao X, Jeong T et al. Novel Specific Pyruvate Kinase M2 Inhibitor, Compound 3h, Induces Apoptosis and Autophagy through Suppressing Akt/mTOR Signaling Pathway in LNCaP Cells Cancers 2022-12-30 [PMID: 36612260]
Marcus A. Toral, Carsten T. Charlesworth, Benjamin Ng, Teja Chemudupati, Shota Homma, Hiromitsu Nakauchi, Alexander G. Bassuk, Matthew H. Porteus, Vinit B. Mahajan Investigation of Cas9 antibodies in the human eye Nature Communications 2022-02-25 [PMID: 35217666]
Uddowla S, Hollister J, Pacheco JM et al. A safe foot-and-mouth disease vaccine platform with two negative markers for differentiating infected from vaccinated animals. J Virol. 2012-11-01 [PMID: 22915802]

Details:
This citation used the HRP form of this antibody.
Munkhjargal T, Aboulaila M, Ueno A et al. Cloning and characterization of histone deacetylase from Babesia bovis. Vet Parasitol. 2012-12-01 [PMID: 22818786]

Details:
This citation used the HRP form of this antibody.
Lin SC, Lin YF, Chong P et al. Broader neutralizing antibodies against H5N1 viruses using prime-boost immunization of hyperglycosylated hemagglutinin DNA and virus-like particles. PLoS One. 2012-01-01 [PMID: 22720032]

Details:
This citation used the HRP form of this antibody.
Sachais BS, Litvinov RI, Yarovoi SV et al. Dynamic antibody-binding properties in the pathogenesis of HIT. Blood. 2012-08-01 [PMID: 22577175]

Details:
This citation used the HRP form of this antibody.
Settem RP, El-Hassan AT, Honma K et al. Fusobacterium nucleatum and Tannerella forsythia induce synergistic alveolar bone loss in a mouse periodontitis model. Infect Immun. 2012-07-01 [PMID: 22547549]

Details:
This citation used the HRP form of this antibody.
Sato K, Imai Y, Higashi N et al. Lack of antigen-specific tissue remodeling in mice deficient in the macrophage galactose-type calcium-type lectin 1/CD301a. Blood. 2005-07-01 [PMID: 15784728]

Details:
This citation used the HRP form of this antibody.
Show All 13 Publications.

Review for IgG Fc fragment Antibody (NB7561) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB7561:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot IgG Fc NB7561
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5
reviewed by:
Verified Customer
WB Human 11/12/2014
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Summary

ApplicationWestern Blot
Sample TestedHuh7 whole cell lysate
SpeciesHuman

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG Fc fragment Antibody (NB7561) (0)

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Verified Customer
11/12/2014
Application: WB
Species: Human