| Reactivity | PmSpecies Glossary |
| Applications | WB, DB, ELISA, ICC/IF, WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | LYOPH |
| Description | The lyophilized IgG fraction is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. |
| Immunogen | Purified Fab from normal IgG isolated from pooled Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | The antiserum reacts with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. The presence of activity to the common Fab subunit may result in the staining of Ig bound to Fc-receptors on non-lymphoid cells. |
| Clonality | Polyclonal |
| Host | Rabbit |
| Purity | Ion exchange chromatography |
| Dilutions |
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| Application Notes | Indirect immunofluorescence. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.2) |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Ion exchange chromatography |
| Reconstitution Instructions | Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C. |
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