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IGFBP-2 Antibody (CL13565)

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Staining of human kidney shows strong membranous positivity in cells in tubules and cells in glomeruli with additional cytoplasmic positivity.
Staining of human kidney shows strong membranous positivity in cells in tubules and cells in glomeruli with additional cytoplasmic positivity.
Orthogonal Strategies: Analysis in human pancreas and skin tissues using NBP3-24569 antibody. Corresponding IGFBP2 RNA-seq data are presented for the same tissues.
Staining of human stomach shows strong membranous positivity in glandular cells.
Staining of human endometrium shows moderate membranous positivity in glandular cells and endometrial stromal cells.
Staining of human pancreas shows moderate membranous positivity in exocrine glandular cells.
Staining of human skin shows negative to very weak positivity in squamous epithelial cells.
Staining of human ovarian cancer (papillary carcinoma) shows strong membranous positivity in tumor cells.
Staining of human endometrial cancer shows moderate membranous positivity in tumor cells.
Staining of human ovarian cancer (low grade serous ovarian carcinoma) shows strong membranous positivity in tumor cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
CL13565
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

  

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IGFBP-2 Antibody (CL13565) Summary

Immunogen
This antibody was designed to target the recombinant protein IGFBP-2, with the precise immunogen sequence being withheld for proprietary reasons.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
IGFBP2
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Purity
Protein A purified

Alternate Names for IGFBP-2 Antibody (CL13565)

  • BP2
  • IBP2
  • IBP-2
  • IGF-binding protein 2
  • IGFBP2
  • IGFBP-2
  • IGF-BP53
  • insulin-like growth factor binding protein 2 (36kD)
  • insulin-like growth factor binding protein 2, 36kDa
  • insulin-like growth factor-binding protein 2

Background

IGFBP2 is a member of the ISGBP family which bind various IGFs. IGFBP2 is over expressed in a wide spectrum of other cancers, including glioma, prostate cancer, synovial sarcoma, neuroblastoma, colon cancer, adrenocortical cancer, lung cancer, Wilms' tumor, and hepatoblastoma. The over expression of IGFBP2 also correlates with the aggressiveness of some tumors. IGFBP2 activates the expression of matrix metalloprotease 2, which contributes to cell invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol IGFBP2