Novus Biologicals products are now on bio-techne.com

Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]

Images

 
Western Blot: Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] [NB7346] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] Summary

Immunogen
Dog IgE
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species. There was less than 0.01% reactivity with purified dog IgG1, IgG2, IgA and IgM.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000 - 1:20000
Readout System
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB7346 in the following applications:

Publications
Read Publications using
NB7346 in the following applications:

Reactivity Notes

Use in Borreliella burgdorferi reported in secitific publication PMID: 32792093

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]

  • Constant Region of Heavy Chain of IgE
  • Ig Epsilon Chain C Region ND
  • Ig Epsilon Chain C Region
  • IgE
  • Immunoglobulin Epsilon
  • immunoglobulin heavy constant epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgE Heavy Chain Antibody (NB7346)(13)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: ELISA.


Filter By Application
ELISA
(2)
All Applications
Filter By Species
Bacteria
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NB7346 Applications Species
Hatke A, Green D, Stasiak K, Marconi R Antibody profiling of a Borreliella burgdorferi (Lyme disease) C6 antibody positive, symptomatic Rottweiler and her pups The Veterinary Journal Aug 1 2020 [PMID: 32792093] (ELISA, Bacteria)

Details:
Borreliella burgdorferi
ELISA Bacteria
Elisa Maina, Bert Devriendt, Eric Cox Food allergen-specific sublingual immunotherapy modulates peripheral T cell responses of dogs with adverse food reactions. Veterinary immunology and immunopathology 2019-08-08 [PMID: 31213250]
Oida K, Einhorn L, Herrmann I et al. Innate function of house dust mite allergens: robust enzymatic degradation of extracellular matrix at elevated pH World Allergy Organ J 2017-07-14 [PMID: 28702111]
Maina E, Pelst M, Hesta M et al. Food-specific sublingual immunotherapy is well tolerated and safe in healthy dogs: a blind, randomized, placebo-controlled study BMC Vet Res 2017-01-20 [PMID: 28100257] (ELISA) ELISA
Maina E, Cox E. A double blind, randomized, placebo controlled trial of the efficacy, quality of life and safety of food allergen-specific sublingual immunotherapy in client owned dogs with adverse food reactions: a small pilot study Vet. Dermatol. [PMID: 27426929]

Details:
Citation using the HRP form of this antibody.
Kubota S, Miyaji K, Shimo Y et al. IgE reactivity to a Cry j 3, an allergen of Japanese cedar (Cryptomeria japonica) pollen in dogs with canine atopic dermatitis. Vet Immunol Immunopathol. [PMID: 22749702]

Details:
Citation using the HRP form of this antibody.
Loukas A, Bethony JM, Mendez S et al. Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine. [PMID: 16231975]

Details:
Citation using the HRP form of this antibody.
Almeida MA, Jesus EE, Sousa-Atta ML et al. Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi. Vet Immunol Immunopathol. [PMID: 15911002]

Details:
Citation using the HRP form of this antibody.
Iniesta L, Gallego M, Portus M. Immunoglobulin G and E responses in various stages of canine leishmaniosis. Vet Immunol Immunopathol. [PMID: 15626463]

Details:
Citation using the HRP form of this antibody.
Moreno M, Benavidez U, Carol H et al. Local and systemic immune responses to Echinococcus granulosus in experimentally infected dogs. Vet Parasitol. [PMID: 15036575]

Details:
Citation using the HRP form of this antibody.
Show All 13 Publications.

Reviews for IgE Heavy Chain Antibody (NB7346) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Canine.

Reviews using NB7346:
Filter by Applications
ELISA
(1)
All Applications
Filter by Species
Canine
(1)
All Species
Images Ratings Applications Species Date Details
 IgE Heavy Chain NB7346
Enlarge
5
reviewed by:
Verified Customer
01/31/2017
View

Summary

LotP36

Comments

CommentsWestern blot on nitrocellulose membrane.
Sample: 0.5µg dog IgE
Blocking: 5% skimmed milk powder in TBS +0.1% Tween-20
Detection: NB7346, 1:8000 diluted in 5% skimmed milk powder in TBS +0.1% Tween-20.
Substrate: Clarity™ ECL Western Blotting Substrate
Exposure time: 5 sec
  4
reviewed by:
elisa maina
ELISA Canine 01/20/2017
View

Summary

ApplicationELISA
Sample TestedDog whole blood
SpeciesCanine

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgE Heavy Chain Antibody (NB7346) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgE Heavy Chain Products

Research Areas for IgE Heavy Chain Antibody (NB7346)

Find related products by research area.

Blogs on IgE Heavy Chain

There are no specific blogs for IgE Heavy Chain, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

Verified Customer
01/31/2017
Application:
Species:

 
elisa maina
01/20/2017
Application: ELISA
Species: Canine