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Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]

 (12 Publications)
 (2 Reviews)
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Western Blot: Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] [NB7346] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. Image collected and cropped by CiteAb from the following publication (//waojournal.biomedcentral.com/articles/10.1186/s40413-017-0154-3), licensed under a CC-BY license.
Western Blot: Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] [NB7346] - D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for ...read more
D.p. extracts have effects on MCs though the IgE-FcepsilonRI axis. A) SDS-PAGE of recombinants (300 ng) and Western blotting (168.8 ng, for rDer p 15; 143.8 ng of rDer p 18 per gel lane) of specific IgE in canine sera. ...read more

Product Details

Summary
Reactivity CaSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
HRP
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
FITC
NB7345
Unconjugated
NB7343
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Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP] Summary

Immunogen
Dog IgE
Specificity
By immunoelectrophoresis and ELISA this reacts specifically with dog IgE. This may cross react with IgE from other species. There was less than 0.01% reactivity with purified dog IgG1, IgG2, IgA and IgM.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • ELISA 1:10000 - 1:100000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:2000
  • Immunohistochemistry 1:200 - 1:2000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000 - 1:20000
Readout System
Reviewed Applications
Read 2 Reviews rated 4.5
using
NB7346 in the following applications:
Publications
Read Publications using
NB7346 in the following applications:

Reactivity Notes

Use in Borreliella burgdorferi reported in secitific publication PMID: 32792093

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Phosphate Buffered Saline (PBS) containing 0.2%BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Goat anti-Canine IgE Heavy Chain Secondary Antibody [HRP]

  • Constant Region of Heavy Chain of IgE
  • Ig Epsilon Chain C Region ND
  • Ig Epsilon Chain C Region
  • IgE
  • Immunoglobulin Epsilon
  • immunoglobulin heavy constant epsilon

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgE Heavy Chain Antibody (NB7346)(12)

We have publications tested in 1 confirmed species: Bacteria.

We have publications tested in 1 application: ELISA.


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ELISA
(2)
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Bacteria
(1)
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Showing Publications 1 - 10 of 12. Show All 12 Publications.
Publications using NB7346 Applications Species
Hatke A, Green D, Stasiak K, Marconi R Antibody profiling of a Borreliella burgdorferi (Lyme disease) C6 antibody positive, symptomatic Rottweiler and her pups The Veterinary Journal Aug 1 2020 [PMID: 32792093] (ELISA, Bacteria)

Details:
Borreliella burgdorferi 		ELISA Bacteria
Oida K, Einhorn L, Herrmann I et al. Innate function of house dust mite allergens: robust enzymatic degradation of extracellular matrix at elevated pH World Allergy Organ J 2017-07-14 [PMID: 28702111]
Maina E, Pelst M, Hesta M et al. Food-specific sublingual immunotherapy is well tolerated and safe in healthy dogs: a blind, randomized, placebo-controlled study BMC Vet Res 2017-01-20 [PMID: 28100257] (ELISA) ELISA
Maina E, Cox E. A double blind, randomized, placebo controlled trial of the efficacy, quality of life and safety of food allergen-specific sublingual immunotherapy in client owned dogs with adverse food reactions: a small pilot study Vet. Dermatol. [PMID: 27426929]

Details:
Citation using the HRP form of this antibody.
Kubota S, Miyaji K, Shimo Y et al. IgE reactivity to a Cry j 3, an allergen of Japanese cedar (Cryptomeria japonica) pollen in dogs with canine atopic dermatitis. Vet Immunol Immunopathol. [PMID: 22749702]

Details:
Citation using the HRP form of this antibody.
Loukas A, Bethony JM, Mendez S et al. Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. PLoS Medicine. [PMID: 16231975]

Details:
Citation using the HRP form of this antibody.
Almeida MA, Jesus EE, Sousa-Atta ML et al. Antileishmanial antibody profile in dogs naturally infected with Leishmania chagasi. Vet Immunol Immunopathol. [PMID: 15911002]

Details:
Citation using the HRP form of this antibody.
Iniesta L, Gallego M, Portus M. Immunoglobulin G and E responses in various stages of canine leishmaniosis. Vet Immunol Immunopathol. [PMID: 15626463]

Details:
Citation using the HRP form of this antibody.
Moreno M, Benavidez U, Carol H et al. Local and systemic immune responses to Echinococcus granulosus in experimentally infected dogs. Vet Parasitol. [PMID: 15036575]

Details:
Citation using the HRP form of this antibody.
Klipper E, Sklan D, Friedman A. Maternal antibodies block induction of oral tolerance in newly hatched chicks. Vaccine. [PMID: 14670332]

Details:
Citation using the HRP form of this antibody.
Show All 12 Publications.

Reviews for IgE Heavy Chain Antibody (NB7346) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 review tested in 1 species: Canine.

Reviews using NB7346:
Filter by Applications
ELISA
(1)
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Canine
(1)
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Images Ratings Applications Species Date Details
IgE Heavy Chain NB7346
Enlarge 		5
reviewed by:
Verified Customer
 01/31/2017
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Summary

LotP36

Comments

CommentsWestern blot on nitrocellulose membrane.
Sample: 0.5µg dog IgE
Blocking: 5% skimmed milk powder in TBS +0.1% Tween-20
Detection: NB7346, 1:8000 diluted in 5% skimmed milk powder in TBS +0.1% Tween-20.
Substrate: Clarity™ ECL Western Blotting Substrate
Exposure time: 5 sec
  4
reviewed by:
elisa maina
 ELISA Canine 01/20/2017
View

Summary

ApplicationELISA
Sample TestedDog whole blood
SpeciesCanine

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgE Heavy Chain Antibody (NB7346) (0)

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Recent Reviews

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IgE Heavy Chain NB7346
Enlarge
 Verified Customer
01/31/2017
Application:
Species:
read this review
 
 elisa maina
01/20/2017
Application: ELISA
Species: Canine
read this review