Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP.
Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.
Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP.
Alternate Names for His Tag Antibody (AD1.1.10) [HRP]
6 His epitope tag
6-His Tag
6X His Tag
6X His
6x-His Tag
6X-His
H
Hexa His tag
HHHHHH epitope tag
HHHHHH tag
His Tag
HIS
His6-Tag
polyhistidine tag
polyHistidine
Poly-histidine
Background
A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).
References
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Publications for His Tag Antibody (NB100-63173)(3)
FAQs for His Tag Antibody (NB100-63173). (Showing 1 - 1 of 1 FAQs).
Does this antibody recognize an internal His6 tag as well as a terminal his6 tag?
This antibody has only been tested to recognize 6-His tags at the N- and C- terminals. I do not know of a situation in which you would have a 6-His tag internally in a protein, but I would suspect this antibody will recognize the tag internally, as long as it is not covered up by the proteins tertiary structure.
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![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-AD1-1-10-HRP-Western-Blot-NB100-63173-img0007.jpg "Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - HIS-tagged protein probed with Mouse anti Histidine tag visualised with Rabbit anti Mouse IgG : HRP.")
![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-AD1-1-10-HRP-Western-Blot-NB100-63173-img0005.jpg "Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - His-Ubiquitin recombinant protein probed with Mouse anti Histidine Tag.")
![Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP.](http://images.novusbio.com/fullsize/6-Histidine-Epitope-Tag-Antibody-AD1-1-10-HRP-Western-Blot-NB100-63173-img0006.jpg "Western Blot: 6-Histidine Epitope Tag Antibody (AD1.1.10) [HRP] [NB100-63173] - Western blot of HIS tagged protein probed with Mouse anti Histidine tag : Biotin and visualised with Streptavidin : HRP.")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")