HIF-2 alpha/EPAS1 Antibody (ep190b)

Hypoxia contributes to the pathophysiology of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease (1). In cancer, and particularly solid tumors, hypoxia plays a critical role in the regulation of genes involved in stem cell renewal, epithelial to mesenchymal transition (EMT), metastasis and angiogenesis. In the tumor microenvironment (TME), hypoxia influences the properties and function of stromal cells (e.g., fibroblasts, endothelial and immune cells) and is a strong determinant of tumor progression (2,3).

HIF-1 or hypoxia inducible factor 1, is a transcription factor commonly referred to as a "master regulator of the hypoxic response" for its central role in the regulation of cellular adaptations to hypoxia. Similarly, HIF-2 alpha plays a role in cellular responses to hypoxia, but whereas HIF-1 alpha is ubiquitously expressed, HIF-2 alpha is predominantly expressed in the vascular endothelium at embryonic stages and after birth in select cells and tissue types (e.g., fibroblasts, hepatocytes and myocytes at 96kDa) (4). Following a similar mechanism to HIF-1 alpha, HIF-2 alpha is stabilized under hypoxic conditions by the formation of a heterodimer with an ARNT/HIF-1 beta subunit. Stable HIF-2 alpha-ARNT/HIF-1 beta heterodimers engage p300/CBP in the nucleus for binding to hypoxic response elements (HREs), inducing transcription, and thus regulation of genes (e.g., EPO, VEGFA). HIF-1 predominantly transactivates genes involved in glycolytic control and pro- apoptotic genes (e.g., LDHA and BNIP3), and HIF-2 regulates the expression of genes involved in invasion and stemness (e.g., MMP2, and OCT4). Common gene targets for HIF-1 and HIF-2 include VEGFA and GLUT1 (5).

The HIF-2 alpha subunit is rapidly targeted and degraded by the ubiquitin proteasome system under normoxic conditions. This process is mediated by oxygen-sensing enzymes, prolyl hydroxylase domain enzymes (PHDs), which catalyze the hydroxylation of key proline residues (Pro-405 and Pro-531) within the oxygen-dependent degradation domain of HIF-2 alpha (5). Once hydroxylated, HIF-2 alpha binds the von Hippel-Lindau tumor suppressor protein (pVHL) for subsequent ubiquitination and proteasomal degradation (5,6).

References

1. Semenza, G. L., Agani, F., Feldser, D., Iyer, N., Kotch, L., Laughner, E., & Yu, A. (2000). Hypoxia, HIF-1, and the pathophysiology of common human diseases. Advances in Experimental Medicine and Biology.

2.Muz, B., de la Puente, P., Azab, F., & Azab, A. K. (2015). The role of hypoxia in cancer progression, angiogenesis, metastasis, and resistance to therapy. Hypoxia. https://doi.org/10.2147/hp.s93413

3. Huang, Y., Lin, D., & Taniguchi, C. M. (2017). Hypoxia inducible factor (HIF) in the tumor microenvironment: friend or foe? Science China Life Sciences. https://doi.org/10.1007/s11427-017-9178-y

4. Hu, C.-J., Wang, L.-Y., Chodosh, L. A., Keith, B., & Simon, M. C. (2003). Differential Roles of Hypoxia-Inducible Factor 1 (HIF-1) and HIF-2 in Hypoxic Gene Regulation. Molecular and Cellular Biology. https://doi.org/10.1128/mcb.23.24.9361-9374.2003

5. Koh, M. Y., & Powis, G. (2012). Passing the baton: The HIF switch. Trends in Biochemical Sciences. https://doi.org/10.1016/j.tibs.2012.06.004

6. Koyasu, S., Kobayashi, M., Goto, Y., Hiraoka, M., & Harada, H. (2018). Regulatory mechanisms of hypoxia-inducible factor 1 activity: Two decades of knowledge. Cancer Science. https://doi.org/10.1111/cas.13483

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Hamster.

We have publications tested in 15 applications: Chemotaxis, ELISA, EMSA, FLOW, ICC/IF, IF/IHC, IHC-Fr, IHC-P, IP, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, KD, WB, Western Blot.

Showing Publications 1 - 10 of 192. Show All 192 Publications. Collapse Publications.

Publications using NB100-132	Applications	Species
Hyunbum Kim, Yu Liu, Jiwon Kim, Yunhye Kim, Timothy Klouda, Sudeshna Fisch, Seung Han Baek, Tiffany Liu, Suzanne Dahlberg, Cheng-Jun Hu, Wen Tian, Xinguo Jiang, Kosmas Kosmas, Helen A Christou, Benjamin D Korman, Sara O Vargas, Joseph C Wu, Kurt R Stenmark, Vinicio de Jesus Perez, Mark R Nicolls, Benjamin A Raby, Ke Yuan Pericytes contribute to pulmonary vascular remodeling via HIF2 alpha signaling EMBO Reports 2024-01-19 [PMID: 38243138]
R Berggren-N, M Ryde, A Löfdahl, A Ibáñez-Fon, M Kåredal, G Westergren, E Tufvesson, AK Larsson-Ca Effects of hypoxia on bronchial and alveolar epithelial cells linked to pathogenesis in chronic lung disorders Frontiers in Physiology, 2023-03-13;14(0):1094245. 2023-03-13 [PMID: 36994416]
Garcia JA, Chen R, Xu M et al. Acss2/HIF-2 signaling facilitates colon cancer growth and metastasis PloS one 2023-03-02 [PMID: 36862715] (Immunoprecipitation, Western Blot, Human)	Immunoprecipitation, Western Blot	Human
Akahori D, Inui N, Inoue Y et al. Effect of Hypoxia on Pulmonary Endothelial Cells from Bleomycin-Induced Pulmonary Fibrosis Model Mice International journal of molecular sciences 2022-08-12 [PMID: 36012260] (ICC/IF, Mouse)	ICC/IF	Mouse
Moyama C, Fujita M, Ando S et al. Stat5b inhibition blocks proliferation and tumorigenicity of glioblastoma stem cells derived from a de novo murine brain cancer model American journal of cancer research 2022-03-15 [PMID: 35411230] (IF/IHC, Human)	IF/IHC	Human
Zhou X, Zheng Y, Sun W et al. D-mannose alleviates osteoarthritis progression by inhibiting chondrocyte ferroptosis in a HIF-2?-dependent manner Cell Proliferation 2021-11-01 [PMID: 34561933] (Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence)	Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence
Rodriguez L, Duchez P, Touya N et al. alpha-Tocopherol Attenuates Oxidative Phosphorylation of CD34+ Cells, Enhances Their G0 Phase Fraction and Promotes Hematopoietic Stem and Primitive Progenitor Cell Maintenance Biomolecules 2021-04-10 [PMID: 33920203] (WB, Mouse)	WB	Mouse
Kuwano A, Kurokawa M, Kohjima M et al. Microcirculatory disturbance in acute liver injury Experimental and therapeutic medicine 2021-06-01 [PMID: 33884034] (IF/IHC, Human)	IF/IHC	Human
Pavlakis D, Kampantais S, Gkagkalidis K et al. Hypoxia-Inducible Factor 2a Expression Is Positively Correlated With Gleason Score in Prostate Cancer Technology in cancer research & treatment 2021-03-23 [PMID: 33752529] (IHC-P, Human)	IHC-P	Human
Ivan Menendez-Montes, Beatriz Escobar, Manuel J. Gomez, Teresa Albendea-Gomez, Beatriz Palacios, Elena Bonzon-Kulichenko, Jose Luis Izquierdo-Garcia, Ana Vanessa Alonso, Alessia Ferrarini, Luis Jesus Jimenez-Borreguero, Jesus Ruiz-Cabello, Jesus Vázquez, Silvia Martin-Puig Activation of amino acid metabolic program in cardiac HIF1-alpha-deficient mice iScience 2021-02-03 [PMID: 33665549]
Lawler AJ, Brown AR, Bouchard RS et al. Cell Type-Specific Oxidative Stress Genomic Signatures in the Globus Pallidus of Dopamine-Depleted Mice The Journal of neuroscience : the official journal of the Society for Neuroscience 2020-12-09 [PMID: 33188066] (IF/IHC, Mouse)	IF/IHC	Mouse
Niklasson, C U, Fredlund, E Et al. Hypoxia inducible factor-2 alpha importance for migration, proliferation, and self-renewal of trunk neural crest cells. Dev Dyn 2021-02-01 [PMID: 32940375] (WB, Mouse)	WB	Mouse
AlbiNana V, Gallardo-Vara E, de Rojas-P I et al. Targeting beta 2-Adrenergic Receptors Shows Therapeutical Benefits in Clear Cell Renal Cell Carcinoma from Von Hippel-Lindau Disease Journal of clinical medicine 2020-08-25 [PMID: 32854260] (IF/IHC, ICC/IF, IHC-P, WB, Human, Mouse)	IF/IHC, ICC/IF, IHC-P, WB	Human, Mouse
Liu PJ, Harris JM, Marchi E et al. Hypoxic gene expression in chronic hepatitis B virus infected patients is not observed in state-of-the-art in vitro and mouse infection models Sci Rep 2020-08-24 [PMID: 32839523]
Hulley PA, Papadimitriou-Olivgeri I, Knowles HJ Osteoblast-osteoclast co-culture amplifies inhibitory effects of FG-4592 on osteoclast formation and reduces bone resorption activity JBMR Plus 2020-07-16 [PMID: 32666021]
Hong K, Hu L, Liu X et al. USP37 promotes deubiquitination of HIF2 alpha in kidney cancer Proc. Natl. Acad. Sci. U.S.A. 2020-05-27 [PMID: 32461361] (WB, Human)	WB	Human
Gkagkalidis K, Kampantais S, Dimitriadis G et Al. Expression of HIF-2a in clear-cell renal cell carcinoma independently predicts overall survival Med Mol Morphol 2020-03-26 [PMID: 32219604] (Human)		Human
Strassheim D, Karoor V, Nijmeh H et al. c-Jun, Foxo3a, and c-Myc Transcription Factors are Key Regulators of ATP-Mediated Angiogenic Responses in Pulmonary Artery Vasa Vasorum Endothelial Cells Cells 2020-02-11 [PMID: 32054096] (KD, WB, Bovine)	KD, WB	Bovine
Persson CU, von Stedingk K, Fredlund E et al. ARNT-dependent HIF-2 transcriptional activity is not sufficient to regulate downstream target genes in neuroblastoma Exp. Cell Res. 2020-01-13 [PMID: 31945318] (IHC-P, Human)	IHC-P	Human
Bacci L, Aiello A, Ripoli C et al. H19-Dependent Transcriptional Regulation of beta 3 and beta 4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer Int J Mol Sci 2019-08-17 [PMID: 31426484] (WB, Chemotaxis)	WB, Chemotaxis
Tang K, Yu Y, Zhu L et al. Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells Oncogene 2019-08-13 [PMID: 31409901] (WB, Mouse)	WB	Mouse
Mallikarjuna P, Raviprakash TS, Aripaka K et al. Interactions between TGF-beta type I receptor and hypoxia-inducible factor-alpha mediates a synergistic crosstalk leading to poor prognosis for patients with clear cell renal cell carcinoma Cell Cycle 2019-09-01 [PMID: 31339433]
Das B, Pal B, Bhuyan R et al. MYC Regulates the HIF2 alpha Stemness Pathway via Nanog and Sox2 to Maintain Self-Renewal in Cancer Stem Cells versus Non-Stem Cancer Cells Cancer Res. 2019-07-02 [PMID: 31266772] (IF/IHC, WB, FLOW, Mouse, Human)	IF/IHC, WB, FLOW	Mouse, Human
Knowles HJ The Adenosine A2B Receptor Drives Osteoclast-Mediated Bone Resorption in Hypoxic Microenvironments Cells 2019-06-21 [PMID: 31234425] (WB, Human)	WB	Human
Duan LJ, Fong GH. Developmental vascular pruning in neonatal mouse retinas is programmed in the astrocytic oxygen sensing mechanism Development 2019-03-25 [PMID: 30910827] (WB, Mouse)	WB	Mouse
Liu S, Zhang G, Guo J et al. Loss of Phd2 cooperates with BRAFV600E to drive melanomagenesis Nat Commun 2018-12-21 [PMID: 30575721]
Sasagawa T, Nagamatsu T, Morita K et al. HIF-2a, but not HIF-1a, mediates hypoxia-induced up-regulation of Flt-1 gene expression in placental trophoblasts. Sci Rep. 2018-11-26 [PMID: 30478339] (WB, Human)	WB	Human
Krueger K, Catanese L, Sciesielski LK et al. Deletion of an intronic HIF-2a binding site suppresses hypoxia-induced WT1 expression. Biochim Biophys Acta Gene Regul Mech. 2018-11-20 [PMID: 30468780] (Chemotaxis, Human)	Chemotaxis	Human
Liu N, Luo J, Kuang D et al. Lactate inhibits ATP6V0d2 expression in tumor-associated macrophages to promote HIF-2a-mediated tumor progression. J. Clin. Invest. 2018-11-15 [PMID: 30431439] (Mouse)		Mouse
Han JS, Lee JH, Kong J et al. Hypoxia Restrains Lipid Utilization via Protein Kinase A and ATGL Downregulation through Hypoxia Inducible Factor. Mol. Cell. Biol. 2018-11-05 [PMID: 30397073] (WB, Mouse)	WB	Mouse
Moreno Roig E, Yaromina A, Houben R et al. Prognostic Role of Hypoxia-Inducible Factor-2alpha Tumor Cell Expression in Cancer Patients: A Meta-Analysis Front Oncol 2018-06-11 [PMID: 29942795] (IHC-P, Human)	IHC-P	Human
M Koeppen, JW Lee, SW Seo, KS Brodsky, S Kreth, IV Yang, PM Buttrick, T Eckle, HK Eltzschig Hypoxia-inducible factor 2-alpha-dependent induction of amphiregulin dampens myocardial ischemia-reperfusion injury Nat Commun, 2018-02-26;9(1):816. 2018-02-26 [PMID: 29483579]
Lin Z, Liu F, Shi P et al. Fatty acid oxidation promotes reprogramming by enhancing oxidative phosphorylation and inhibiting protein kinase C. Stem Cell Res Ther. 2018-02-26 [PMID: 29482657] (WB, Mouse)	WB	Mouse
Liu Y, Tan X, Liu W, Chen X. Follistatin-like protein 1 plays a tumor suppressor role in clear-cell renal cell carcinoma. Chin J Cancer. 2018-01-22 [PMID: 29357946] (IHC-P, Human)	IHC-P	Human
Chen R, Xu M, Nagati J, Garcia JA. Coordinate regulation of stress signaling and epigenetic events by Acss2 and HIF-2 in cancer cells. PLoS ONE 2017-12-27 [PMID: 29281714] (Chemotaxis, WB, IP, Human)	Chemotaxis, WB, IP	Human
Carew JS, Espitia CM, Zhao W et al. Oncolytic reovirus inhibits angiogenesis through induction of CXCL10/IP-10 and abrogation of HIF activity in soft tissue sarcomas Oncotarget. 2017-10-17 [PMID: 29156834] (WB)	WB
Sadiku P, Willson JA, Dickinson RS et al. Prolyl hydroxylase 2 inactivation enhances glycogen storage and promotes excessive neutrophilic responses J. Clin. Invest. 2017-08-14 [PMID: 28805660] (IHC-P, Mouse)	IHC-P	Mouse
Padro M, Louie RJ, Lananna BV et al. Genome-independent hypoxic repression of estrogen receptor alpha in breast cancer cells. BMC Cancer. 2017-03-20 [PMID: 28320353] (WB, ICC/IF, Human)	WB, ICC/IF	Human
Liu W, Chen H, Wong N et al. Pseudohypoxia induced by miR-126 deactivation promotes migration and therapeutic resistance in renal cell carcinoma. Cancer Lett. 2017-05-28 [PMID: 28257806] (WB, Human)	WB	Human
Shiraishi A, Tachi K, Essid N et al. Hypoxia promotes the phenotypic change of aldehyde dehydrogenase activity of breast cancer stem cells. Cancer Sci. 2017-03-01 [PMID: 28012234] (WB, Human)	WB	Human
Endo H, Okami J, Okuyama H et al. The induction of MIG6 under hypoxic conditions is critical for dormancy in primary cultured lung cancer cells with activating EGFR mutations. Oncogene. 2016-11-28 [PMID: 27893711] (WB, Human)	WB	Human
Leszczynska KB, Dobrynin G, Leslie RE et al. Preclinical testing of an Atr inhibitor demonstrates improved response to standard therapies for esophageal cancer. Radiother Oncol. 2016-11-01 [PMID: 27839769] (WB, Human)	WB	Human
Di Stefano V, Torsello B, et al. Major Action of Endogenous Lysyl Oxidase in Clear Cell Renal Cell Carcinoma Progression and Collagen Stiffness Revealed by Primary Cell Cultures. Am J Pathol 2016-09-01 [PMID: 27449199] (WB, Human)	WB	Human
Kwak JH, Lee NH, Lee HY et al. HIF2a/EFEMP1 cascade mediates hypoxic effects on breast cancer stem cell hierarchy Oncotarget 2016-07-12 [PMID: 27270657] (WB, Mouse)	WB	Mouse
Szendroi A, Szasz AM, Kardos M et al. Opposite prognostic roles of HIF1alpha and HIF2alpha expressions in bone metastatic clear cell renal cell cancer. Oncotarget. 2016-05-27 [PMID: 27244898] (IF/IHC) Details: This citation used the HRP version of this antibody.	IF/IHC
Nusken E, Wohlfarth M, Lippach G et al. Reduced Perinatal Leptin Availability May Contribute to Adverse Metabolic Programming in a Rat Model of Uteroplacental Insufficiency. Endocrinology. 2016-05-01 [PMID: 27007072] Details: This citation used the HRP version of this antibody.
Fan Y, Li H, Ma X et al. Dicer suppresses the malignant phenotype in VHL-deficient clear cell renal cell carcinoma by inhibiting HIF-2alpha. Oncotarget 2016-04-05 [PMID: 26943772]
Chul Ju Hwang, Mi Hee Park, Jae Yeon Hwang, Ju Hwan Kim, Na Young Yun, Sang Yeon Oh, Ju Kyung Song, Hyun Ok Seo, Yun-Bae Kim, Dae Yeon Hwang, Ki-Wan Oh, Sang-Bae Han, Jin Tae Hong CCR5 deficiency accelerates lipopolysaccharide-induced astrogliosis, amyloid-beta deposit and impaired memory function Oncotarget 2016-03-15 [PMID: 26910914]
Espana-Agusti J, Zou X, Wong K et al. Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells. PLoS One 2016-02-11 [PMID: 26866916]
Kim Y, Nam HJ, Lee J et al. Methylation-dependent regulation of HIF-1alpha stability restricts retinal and tumour angiogenesis. Nat Commun 2016-01-13 [PMID: 26757928] (WB)	WB
Lee SB, Frattini V, Bansal M et al. An ID2-dependent mechanism for VHL inactivation in cancer. Nature. 2016-01-14 [PMID: 26735018] (IHC-P, Human)	IHC-P	Human
Su J, Li Z, Cui S et al. The Local HIF-2alpha/EPO Pathway in the Bone Marrow is Associated with Excessive Erythrocytosis and the Increase in Bone Marrow Microvessel Density in Chronic Mountain Sickness High Alt Med Biol [PMID: 26625252] (Human) Details: Used the DyLight 488 form of this antibody.		Human
Seidl CI, Martinez-Sanchez A, Murphy CL. Derepression of miRNA-138 contributes to loss of the human articular chondrocyte phenotype. Arthritis Rheumatol. 2015-09-11 [PMID: 26359943] (WB, Human)	WB	Human
Espana-Agusti J, Tuveson DA, Adams DJ, Matakidou A. A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Sci Rep 2015-01-01 [PMID: 26046460] (IF/IHC, Mouse)	IF/IHC	Mouse
Her YF, Nelson-Holte M, Maher LJ. Oxygen concentration controls epigenetic effects in models of familial paraganglioma. PLoS ONE. 2015-05-19 [PMID: 25985299] (WB, IHC-P, Human, Mouse)	WB, IHC-P	Human, Mouse
Pahlman S, Lund LR, Jogi A. Differential HIF-1alpha and HIF-2alpha Expression in Mammary Epithelial Cells during Fat Pad Invasion, Lactation, and Involution. PLoS ONE. 2015-05-09 [PMID: 25955753] (IHC-P, Mouse)	IHC-P	Mouse
Chen Rui, Xu Min, Nagati Jason S et al. The acetate/ACSS2 switch regulates HIF-2 stress signaling in the tumor cell microenvironment. PLoS One. 2014-01-01 [PMID: 25689462] (IP, WB, Human)	IP, WB	Human
Chen T, Zhou G, Zhou Q et al. Loss of miR-17~92 in Smooth Muscle Cells Attenuates Experimental Pulmonary Hypertension via Induction of PDLIM5 Am. J. Respir. Crit. Care Med. 2015-02-03 [PMID: 25647182] (WB, Human)	WB	Human
Niemi Henna, Honkonen Krista, Korpisalo Petra et al. HIF-1alpha and HIF-2alpha induce angiogenesis and improve muscle energy recovery. Eur J Clin Invest. 2014-10-01 [PMID: 25208310]
Xu M, Nagati JS, Xie J et al. An acetate switch regulates stress erythropoiesis. Nat. Med. 2014-08-10 [PMID: 25108527] (IP, Chemotaxis, WB, Human)	IP, Chemotaxis, WB	Human
Motzer Robert J, Hutson Thomas E, Hudes Gary R et al. Investigation of novel circulating proteins, germ line single-nucleotide polymorphisms, and molecular tumor markers as potential efficacy biomarkers of first-line sunitinib therapy for advanced renal cell carcinoma. Cancer Chemother Pharmacol. 2014-08-07 [PMID: 25100134] (Human)		Human
Evans CE, Wadoodi A, Humphries J et al. Local accumulation of hypoxia-inducible factor 2 alpha during venous thrombus resolution. Thromb. Res. 2014-07-23 [PMID: 25087887] (IHC-P, Mouse) Details: HIF2a/HIF-2 alpha antibody used for IHC-P in venous thrombus induced in the inferior vena cava /IVC of male BALB/C mice treated or not with HIF agonist L-mimosine (Fig 1, 2). Antigen retrieval - pressure cooking sections in citrate buffer (0.3% sodium citrate, pH 6).	IHC-P	Mouse
Wong WJ, Richardson T, Seykora JT et al. Hypoxia-inducible factors regulate filaggrin expression and epidermal barrier function. J Invest Dermatol 2015-02-01 [PMID: 24999590] (Mouse)		Mouse
Peng YJ, Yuan G, Khan S et al. Regulation of Hypoxia Inducible Factor-alpha Isoforms and Redox State By Carotid Body Neural Activity in Rats. J. Physiol. (Lond.). 2014-06-27 [PMID: 24973414] (IHC-Fr, WB, Rat)	IHC-Fr, WB	Rat
Carrera S, Senra J, Acosta MI et al. The Role of the HIF-1alpha Transcription Factor in Increased Cell Division at Physiological Oxygen Tensions. PLoS ONE. 2014-05-19 [PMID: 24835245] (WB, Human)	WB	Human
Toyoda H, Hirayama J, Sugimoto Y et al. Polycythemia and Paraganglioma With a Novel Somatic HIF2A Mutation in a Male. Pediatrics. 2014-05-12 [PMID: 24819565] (IHC-P, Human)	IHC-P	Human
Gregg JL, Turner RM, Chang G et al. NADPH oxidase NOX4 supports renal tumorigenesis by promoting the expression and nuclear accumulation of HIF2alpha. Cancer Res. 2014-04-22 [PMID: 24755467] (WB, IHC-P, ICC/IF, Human)	WB, IHC-P, ICC/IF	Human
Raspaglio G, Petrillo M, Martinelli E et al. Sox9 and Hif-2a regulate TUBB3 gene expression and affect ovarian cancer aggressiveness. Gene 2014-03-21 [PMID: 24661907] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Duan LJ, Takeda K, Fong GH. Hematological, Hepatic, and Retinal Phenotypes in Mice Deficient for Prolyl Hydroxylase Domain (PHD) Proteins in the Liver. Am. J. Pathol. 2014-02-19 [PMID: 24508125] (WB, Mouse)	WB	Mouse
Duan LJ, Takeda K, Fong GH. Hypoxia inducible factor-2 alpha regulates the development of retinal astrocytic network by maintaining adequate supply of astrocyte progenitors. PLoS ONE 2014-01-29 [PMID: 24475033] (WB, Mouse)	WB	Mouse
Ellinghaus P, Heisler I, Unterschemmann K et al. BAY 87-2243, a highly potent and selective inhibitor of hypoxia-induced gene activation has antitumor activities by inhibition of mitochondrial complex I. Cancer Med 2013-10-01 [PMID: 24403227] (WB)	WB
Anderson ER, Taylor M, Xue X et al. Intestinal HIF2alpha promotes tissue-iron accumulation in disorders of iron overload with anemia. Proc Natl Acad Sci U S A. 2013-12-10 [PMID: 24282296] (WB, Mouse)	WB	Mouse
Zhang T, Niu X, Liao L et al. The Contributions of HIF-Target Genes to Tumor Growth in RCC. PLoS One. 2013-11-18 [PMID: 24260413] (WB, Human)	WB	Human
Tsang WP, Shu Y, Kwok PL et al. CD146(+) Human Umbilical Cord Perivascular Cells Maintain Stemness under Hypoxia and as a Cell Source for Skeletal Regeneration. PLoS One. 2013-10-18 [PMID: 24204598] (WB, Human)	WB	Human
Thompson AA, Elks PM, Marriott HM et al. Hypoxia-inducible factor 2alpha regulates key neutrophil functions in humans, mice and zebrafish. Blood. 2013-11-06 [PMID: 24196071] (WB, IF/IHC, Mouse, Human)	WB, IF/IHC	Mouse, Human
Klotzsche-von Ameln A, Prade I, Grosser M et al. Prolyl hydroxylase-4 overexpression stimulates tumor angiogenesis in mouse osteosarcoma via transforming growth factor-alpha. Mol Cancer Res. 2013-09-18 [PMID: 24048703] (WB, Human)	WB	Human
Yeh IJ, Ogba N, Bensigner H et al. HEXIM1 downregulates Hypoxia Inducible Factor-1 alpha protein stability. Biochem J. 2013-09-10 [PMID: 24015760] (WB, Human)	WB	Human
David JM, Owens TA, Barwe SP, Rajasekaran AK. Gramicidin a induces metabolic dysfunction and energy depletion leading to cell death in renal cell carcinoma cells. Mol Cancer Ther. 2013-11-01 [PMID: 24006494]
Bruells CS, Maes K, Rossaint R et al. Prolonged Mechanical Ventilation Alters the Expression Pattern of Angio-neogenetic Factors in a Pre-Clinical Rat Model. PLoS One. 2013-08-08 [PMID: 23950950] (WB, Rat)	WB	Rat
Park YK, Park B, Lee SY et al. Hypoxia-Inducible Factor-2alpha-Dependent Hypoxic Induction of Wnt10b Expression in Adipogenic Cells. J Biol Chem 2013-07-30 [PMID: 23900840] (WB, Mouse)	WB	Mouse
Gottfried E, Lang SA, Renner K et al. New Aspects of an Old Drug - Diclofenac Targets MYC and Glucose Metabolism in Tumor Cells. PLoS One 2013-07-09 [PMID: 23874405] (WB, Human)	WB	Human
Nakajima T, Nakajima E, Shearer TR, Azuma M. Concerted inhibition of HIF-1alpha and -2alpha expression markedly suppresses angiogenesis in cultured RPE cells. Mol Cell Biochem 2013-07-20 [PMID: 23873332] (WB, Human)	WB	Human
Jamil S, Cedervall J, Hultman I et al. Neuroblastoma cells injected into experimental mature teratoma reveal a tropism for embryonic loose mesenchyme. Int J Oncol 2013-09-01 [PMID: 23857308]
Adamski J, Price A, Dive C, Makin G. Hypoxia-induced cytotoxic drug resistance in osteosarcoma is independent of HIF-1Alpha PLoS One 2013-06-21 [PMID: 23785417] (WB, Human) Details: Citation using the Non-Recombinant Monoclonal and Biotin format of this antibody.	WB	Human
Nakayama K. CREB and NF-kB are activated during prolonged hypoxia and cooperatively regulate the induction of matrix metalloproteinase MMP1. J Biol Chem 2013-06-17 [PMID: 23775082] (WB, Human)	WB	Human
Fukui N, Kageyama Y, Higashi Y et al. Development of a novel interferon-alpha2b gene construct with a repetitive hypoxia-inducible factor binding site and its suppressive effects on human renal cell carcinoma cell lines in vitro. Int J Clin Oncol 2013-06-06 [PMID: 23739923] (WB, Human)	WB	Human
Lee WH, Lee JM, Lim C, Kim S, Kim SG et al. Structural requirements within protoporphyrin IX in the inhibition of heat shock protein 90. Chem Biol Interact 2013-04-24 [PMID: 23624237] (WB, Human)	WB	Human
Kucharzewska P, Christianson HC, Welch JE et al. Exosomes reflect the hypoxic status of glioma cells and mediate hypoxia-dependent activation of vascular cells during tumor development. Proc Natl Acad Sci U S A 2013-04-30 [PMID: 23589885] (WB, Human)	WB	Human
Onnis B, Fer N, Rapisarda A et al. Autocrine production of IL-11 mediates tumorigenicity in hypoxic cancer cells J Clin Invest. 2013-04-01 [PMID: 23549086] (WB, Human)	WB	Human
Scheuermann TH, Li Q, Ma HW et al. Allosteric inhibition of hypoxia inducible factor-2 with small molecules. Nat Chem Biol 2013-04-01 [PMID: 23434853] (Chemotaxis, Human)	Chemotaxis	Human
Huang C, Hales BF. Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs PLoS One 2012-01-01 [PMID: 23251655] (IF/IHC, WB, Mouse)	IF/IHC, WB	Mouse
Sun HX, Xu Y, Yang XR et al. Hypoxia inducible factor 2 alpha inhibits hepatocellular carcinoma growth through the transcription factor dimerization partner 3/ E2F transcription factor 1-dependent apoptotic pathway. Hepatology 2012-12-05 [PMID: 23212661] (ICC/IF, Human)	ICC/IF	Human
Macaulay IC, Thon JN, Tijssen MR et al. Canonical Wnt signaling in megakaryocytes regulates proplatelet formation. Blood. 2013-01-03 [PMID: 23160460] Details: WB: Fig 3C (CHRF 288-11 human megarkeryocytic cell line), GARP/LRRC3 was detected at 72 kDA.
Havasi A, Haegele JA, Gall JM et al. Histone Acetyl Transferase (HAT) HBO1 and JADE1 in Epithelial Cell Regeneration Am J Pathol 2012-11-14 [PMID: 23159946]
Persano L, Pistollato F, Rampazzo E et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1 alpha stability and MGMT expression Cell Death Dis 2012-10-18 [PMID: 23076220] (WB, Chemotaxis, Human)	WB, Chemotaxis	Human
Demachi-Okamura A, Torikai H, Akatsuka Y et al. Autophagy Creates a CTL Epitope That Mimics Tumor-Associated Antigens PLoS One 2012-01-01 [PMID: 23071732] (WB, Human)	WB	Human
Xue X, Shah YM. Hypoxia-inducible factor-2 alpha is essential in activating the COX2/mPGES-1/PGE2 signaling axis in colon cancer Carcinogenesis 2012-10-05 [PMID: 23042097] (Chemotaxis, Mouse)	Chemotaxis	Mouse
Vaapil M, Helczynska K, Villadsen R et al. Hypoxic Conditions Induce a Cancer-Like Phenotype in Human Breast Epithelial Cells PLoS One 2012-01-01 [PMID: 23029547] (WB, IF/IHC, Human)	WB, IF/IHC	Human
Thomas S, Harding MA, Smith SC et al. CD24 Is an Effector of HIF-1-Driven Primary Tumor Growth and Metastasis Cancer Res 2012-10-22 [PMID: 22926560] (WB, Human)	WB	Human
Muratsu-Ikeda S, Nangaku M, Ikeda Y et al. Downregulation of miR-205 Modulates Cell Susceptibility to Oxidative and Endoplasmic Reticulum Stresses in Renal Tubular Cells plos One 2012-01-01 [PMID: 22859986] (WB, Human)	WB	Human
Mihaylova S, Killian A, Mayer K et al. Effects of anti-inflammatory vagus nerve stimulation on the cerebral microcirculation in endotoxinemic rats J Neuroinflammation 2012-07-25 [PMID: 22830560] (WB, Rat)	WB	Rat
Chen R, Xu M, Hogg RT et al. The Acetylase/Deacetylase Couple CREB-binding Protein/Sirtuin 1 Controls Hypoxia-inducible Factor 2 Signaling J Biol Chem 2012-08-31 [PMID: 22807441] (IP, Human)	IP	Human
Kiss J, Mollenhauer M, Walmsley SR et al. Loss of the Oxygen Sensor PHD3 Enhances the Innate Immune Response to Abdominal Sepsis J Immunol 2012-08-15 [PMID: 22786772] (WB, Mouse)	WB	Mouse
Lim CS, Kiriakidis S, Paleolog EM, Davies AH. The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent J Vasc Surg 2012-06-05 [PMID: 22677007] (WB, Human)	WB	Human
El Naggar A, Clarkson P, Zhang F et al. Expression and stability of hypoxia inducible factor 1alpha in osteosarcoma Pediatr Blood Cancer 2012-12-15 [PMID: 22544557] (WB, Human)	WB	Human
Jeong CW, Yoon CY, Jeong SJ, Hong SK, Byun SS, Kwak C, Lee SE. The Role of Hypoxia-Inducible Factor-1? and -2? in Androgen Insensitive Prostate Cancer Cells. Urol Oncol. 2012-04-24 [PMID: 22537539] (WB, Human)	WB	Human
Schodel J, Bardella C, Sciesielski LK et al. Common genetic variants at the 11q13.3 renal cancer susceptibility locus influence binding of HIF to an enhancer of cyclin D1 expression Nat Genet 2012-03-11 [PMID: 22406645] (WB, Human)	WB	Human
Conley SJ, Gheordunescu E, Kakarala P, et al. Antiangiogenic agents increase breast cancer stem cells via the generation of tumor hypoxia. Proc Natl Acad Sci U S A. 2012-01-23 [PMID: 22308314] (WB, Mouse)	WB	Mouse
Carew Jennifer S, Esquivel Juan A, Espitia Claudia M et al. ELR510444 inhibits tumor growth and angiogenesis by abrogating HIF activity and disrupting microtubules in renal cell carcinoma. PLoS One 2012-01-01 [PMID: 22295124] (WB, Human)	WB	Human
Munksgaard Persson M, Johansson ME, Monsef N, Planck M, Beckman S, Seckl MJ, RonnstrL, Pahlman S, Pettersson HM. HIF-2? expression is suppressed in SCLC cells, which survive in moderate and severe hypoxia when HIF-1? is repressed. Am J Pathol;180(2):494-504. 2012-02-01 [PMID: 22115707]
Niu X, Zhang T, Liao L et al. The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C. Oncogene 2012-02-09 [PMID: 21725364] (WB)	WB
HollWS, Tepper CG, Pietri JE et al. Evaluating rational non-cross-resistant combination therapy in advanced clear cell renal cell carcinoma: combined mTOR and AKT inhibitor therapy. Cancer Chemother Pharmacol. 2011-06-05 [PMID: 21644050] (WB, Human)	WB	Human
Lin Q, Cong X, Yun Z. Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha Mol Cancer Res 2011-06-01 [PMID: 21571835] (WB, Human)	WB	Human
Heikkila M, Pasanen A, Kivirikko K et al. Roles of the human hypoxia-inducible factor (HIF)-3a variants in the hypoxia response Cell Mol Life Sci 2011-12-01 [PMID: 21479871] (WB, Hamster)	WB	Hamster
Taylor M, Qu A, Anderson ER et al. Hypoxia-inducible factor-2[alpha] mediates the adaptive increase of intestinal ferroportin during iron deficiency in mice. Gastroenterology;140(7):2044-2055. 2011-06-01 [PMID: 21419768] (WB, Chemotaxis, Mouse)	WB, Chemotaxis	Mouse
Xie L, Collins J. Transcriptional regulation of the Menkes copper ATPase Atp7a) gene by hypoxia-inducible factor HIF2{alpha}) in intestinal epithelial cells. Am J Physiol Cell Physiol 2011-01-01 [PMID: 21346155] Details: Using the HRP conjugated version of NB100-132, catalog number NB100-132H.
Chen R, Dioum EM, Hogg RT et al. Hypoxia increases sirtuin 1 expression in a hypoxia-inducible factor-dependent manner J Biol Chem 2011-04-01 [PMID: 21345792] (Chemotaxis, WB, Human)	Chemotaxis, WB	Human
Place TL, Fitzgerald MP, Venkataraman S et al. Aberrant promoter CpG methylation is a mechanism for impaired PHD3 expression in a diverse set of malignant cells. PLoS One. 2011-01-01 [PMID: 21297970] (WB, Human)	WB	Human
Li Q, Michaud M, Canosa S, Kuo A, Madri JA. GSK-3?: a signaling pathway node modulating neural stem cell and endothelial cell interactions. Angiogenesis;14(2):173-85. 2011-05-01 [PMID: 21253820] (WB, Mouse)	WB	Mouse
Nakamura K, Tan F, Li Z, Thiele CJ. NGF activation of TrkA induces vascular endothelial growth factor expression via induction of hypoxia-inducible factor-1alpha. Mol Cell Neurosci 2011-02-01 [PMID: 21145972] (Mouse)		Mouse
Husted RF, Lu H, Sigmund RD, Stokes JB. Oxygen regulation of the epithelial Na channel in the collecting duct. Am J Physiol Renal Physiol 2011-02-01 [PMID: 21123494]
Bougatef F, Menashi S, Khayati F et al. EMMPRIN promotes melanoma cells malignant properties through a HIF-2alpha mediated up-regulation of VEGF-receptor-2 PLoS One 2010-08-31 [PMID: 20824203] (IF/IHC, ICC/IF, WB, Human)	IF/IHC, ICC/IF, WB	Human
Pastorino F, Loi M, Sapra P et al. Tumor regression and curability of preclinical neuroblastoma models by PEGylated SN38 (EZN-2208), a novel topoisomerase I inhibitor. Clin Cancer Res 2010-10-01 [PMID: 20702613] (WB, Human)	WB	Human
Werth N, Beerlage C, Rosenberger C et al. Activation of hypoxia inducible factor 1 is a general phenomenon in infections with human pathogens. PLoS One 2010-01-01 [PMID: 20644645] (IF/IHC)	IF/IHC
Wang X, Schneider A HIF-2alpha-mediated activation of the epidermal growth factor receptor potentiates head and neck cancer cell migration in response to hypoxia. Carcinogenesis 2010-01-07 [PMID: 20395290] (IF/IHC, Human)	IF/IHC	Human
Zimmer M, Lamb J, Ebert BL et al. The connectivity map links iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2. Cancer Res 2010-04-15 [PMID: 20354189] (WB, Human)	WB	Human
Arvidsson Y, Bergstrom A, Arvidsson L et al. Hypoxia stimulates CXCR4 signalling in ileal carcinoids. Endocr Relat Cancer 2010-06-01 [PMID: 20071457] (IF/IHC, Human)	IF/IHC	Human
S K Martin, P Diamond, S A Williams et al. Hypoxia-inducible factor-2 is a novel regulator of aberrant CXCL12 expression in multiple myeloma plasma cells. Haematologica; 95 (5): 776-784. 2010-01-01 [PMID: 20015878]
Kim Y, Lin Q, Zelterman D et al. Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. Cancer Res;69(24):9271-80. 2009-12-15 [PMID: 19934310]
Volke M, Gale DP, Maegdefrau U et al. Evidence for a lack of a direct transcriptional suppression of the iron regulatory peptide hepcidin by hypoxia-inducible factors. PLoS One 2009-11-18 [PMID: 19924283] (WB, Human)	WB	Human
Noguera R, Fredlund E, Piqueras M et al. HIF-1alpha and HIF-2alpha are differentially regulated in vivo in neuroblastoma: high HIF-1alpha correlates negatively to advanced clinical stage and tumor vascularization. Clin Cancer Res;15(23):7130-6. 2009-12-01 [PMID: 19903792]
Abbruzzetti S, Faggiano S, Bruno S et al. Ligand migration through the internal hydrophobic cavities in human neuroglobin. PNAS :0905433106. 2009-01-01 [PMID: 19850865]
Forristal C E, Wright K L et al. Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction 2010-01-01 [PMID: 19755485] (WB, Human)	WB	Human
Lee DW, Rajagopalan S, Siddiq A et al. Inhibition of Prolyl Hydroxylase Protects against 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced Neurotoxicity: MODEL FOR THE POTENTIAL INVOLVEMENT OF THE HYPOXIA-INDUCIBLE FACTOR PATHWAY IN PARKINSON DISEASE. J Biol Chem;284(42):29065-29076. 2009-01-01 [PMID: 19679656]
Bangiyeva V, Rosenbloom A, Alexander AE et al. Differences in regulation of tight junctions and cell morphology between VHL mutations from disease subtypes. BMC Cancer;9:229. 2009-07-14 [PMID: 19602254]
Tuller ER, Beavers CT, Lou JR et al. Docosahexaenoic acid inhibits superoxide dismutase 1 gene transcription in human cancer cells: the involvement of PPAR{alpha} HIF-2{alpha} signaling. Mol Pharmacol :mol.109.057430. 2009-01-01 [PMID: 19528198]
Figlin RA, de Souza P, McDermott D et al. Analysis of PTEN and HIF-1alpha and correlation with efficacy in patients with advanced renal cell carcinoma treated with temsirolimus versus interferon-alpha. Cancer 2009-08-15 [PMID: 19526589] (IHC-P, Human)	IHC-P	Human
Rasheed S, Harris AL, Tekkis PP et al. Hypoxia-inducible factor-1alpha and -2alpha are expressed in most rectal cancers but only hypoxia-inducible factor-1alpha is associated with prognosis. Br J Cancer 2009-05-19 [PMID: 19436307] (IHC-P, Human)	IHC-P	Human
Burkitt K, Chun SY, Dang DT et al. Targeting both HIF-1 HIF-2 in human colon cancer cells improves tumor response to sunitinib treatment. Mol Cancer Ther;8(5):1148-1156. 2009-01-01 [PMID: 19435875]
Ndubuizu OI, Chavez JC, LaManna JC. Increased prolyl 4-hydroxylase expression differential regulation of hypoxia-inducible factors in the aged rat brain. Am J Physiol Regulatory Integrative Comp Physiol;297(1):R158-165. 2009-01-01 [PMID: 19420289]
Li Y, Qiu X, Zhang S et al. Hypoxia induced CCR7 expression via HIF-1alpha and HIF-2alpha correlates with migration and invasion in lung cancer cells. Cancer Biol Ther 2009-02-01 [PMID: 19305150] (Human)		Human
Walter A, Etienne-Selloum N, Brasse D et al. Red Wine Polyphenols Prevent Acceleration of Neovascularization by Angiotensin II in the Ischemic Rat Hindlimb. J Pharmacol Exp Ther;329(2):699-707. 2009-01-01 [PMID: 19193929]
Wacker I, Sachs M, Knaup K et al. A Key Role for Activin B in Cellular Transformation after Loss of the von Hippel-Lindau (VHL) Tumor Suppressor. Mol Cell Biol :MCB01184-01107. 2009-01-01 [PMID: 19158274]
Zimmer M, Ebert BL, Neil C et al. Small-molecule inhibitors of HIF-2a translation link its 5'UTR iron-responsive element to oxygen sensing. Mol Cell 2008-12-26 [PMID: 19111663] (WB, Human)	WB	Human
Imamura T, Kikuchi H, Herraiz MT et al. HIF-1alpha and HIF-2alpha have divergent roles in colon cancer. Int J Cancer 2009-02-15 [PMID: 19030186] (WB, IHC-P, Human)	WB, IHC-P	Human
Westfall SD, Sachdev S, Das P et al. Identification of oxygen-sensitive transcriptional programs in human embryonic stem cells. Stem Cells Dev 2008-10-01 [PMID: 18811242] (ICC/IF, Human)	ICC/IF	Human
Evens AM, Schumacker PT, Helenowski IB et al. Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family. Br J Haematol 2008-05-01 [PMID: 18422776] (IF/IHC, Human)	IF/IHC	Human
Warnecke C, Weidemann A, Volke M et al. The specific contribution of hypoxia-inducible factor-2alpha to hypoxic gene expression in vitro is limited and modulated by cell type-specific and exogenous factors. Exp Cell Res 2008-06-10 [PMID: 18420194] (WB, Human)	WB	Human
Borger DR, Gavrilescu LC, Bucur MC et al. AMP-activated protein kinase is essential for survival in chronic hypoxia. Biochem Biophys Res Commun 2008-05-30 [PMID: 18359290] (WB, Human)	WB	Human
Kubo T, Sugita T, Shimose S et al. Expression of hypoxia-inducible factor-1alpha and its relationship to tumour angiogenesis and cell proliferation in cartilage tumours. J Bone Joint Surg 2008-03-01 [PMID: 18310762] (WB)	WB
Chintalapudi MR, Markiewicz M, Kose N, Dammai V, Champion KJ, Hoda RS, Trojanowska M, Hsu T. Cyr61/CCN1 and CTGF/CCN2 mediate the proangiogenic activity of VHL-mutant renal carcinoma cells. Carcinogenesis;29(4):696-703. 2008-04-01 [PMID: 18212329]
Chan DA, Krieg AJ, Turcotte S, Giaccia AJ. HIF gene expression in cancer therapy. Methods Enzymol 2007-01-01 [PMID: 17998061]
Scheuermann TH, Yang J, Zhang L et al. Hypoxia-inducible factors Per/ARNT/Sim domains: structure and function. Methods Enzymol 2007-01-01 [PMID: 17998046]
Sutphin, PD et al. Tageting the Loss of the von Hippel-Lindau Tumor Suppressor Gene in Renal Cell Carcinoma Cells. Cancer Res67: 5896-5905. 2007-01-01 [PMID: 17575159]
Monsef N, Helczynski L, Lundwall A, Pahlman S et al. Localization of immunoreactive HIF-1alpha and HIF-2alpha in neuroendocrine cells of both benign and malignant prostate glands. Prostate 2007-08-01 [PMID: 17562539] (ICC/IF, Human)	ICC/IF	Human
Rankin EB, Biju MP, Liu Q et al. Hypoxia-inducible factor-2 (HIF-2) regulates hepatic erythropoietin in vivo. J Clin Invest 2007-04-01 [PMID: 17404621] (EMSA, Mouse)	EMSA	Mouse
Lau KW, Tian YM, Raval RR et al. Target gene selectivity of hypoxia-inducible factor-alpha in renal cancer cells is conveyed by post-DNA-binding mechanisms. Br J Cancer 2007-04-23 [PMID: 17387348] (WB, Human)	WB	Human
Harvey AJ, Kind KL, Thompson JG. Regulation of gene expression in bovine blastocysts in response to oxygen and the iron chelator desferrioxamine. Biol Reprod;77(1):93-101. 2007-07-01 [PMID: 17329595] (ICC/IF, Human)	ICC/IF	Human
Bernhardt WM, Wiesener MS, Weidemann A et al. Involvement of hypoxia-inducible transcription factors in polycystic kidney disease. Am J Pathol;170(3):830-42. 2007-03-01 [PMID: 17322369] (IHC-P, Rat)	IHC-P	Rat
Kyriacou HM, Steen KE, Raza A et al. In vitro inhibition of Plasmodium falciparum rosette formation by Curdlan sulfate. Antimicrob Agents Chemother 2007-04-01 [PMID: 17283200] (IF/IHC)	IF/IHC
Yan Q, Bartz S, Mao M, Li L, Kaelin WG Jr. The hypoxia-inducible factor 2alpha N-terminal and C-terminal transactivation domains cooperate to promote renal tumorigenesis in vivo. Mol Cell Biol;27(6):2092-102. 2007-03-01 [PMID: 17220275]
Koizume, S et al. (2006) Activation of cancer cell migration and invasion by ectopic synthesis of coagulation factor VII. Cancer Res;66(19):9453-60. 2006-10-01 [PMID: 17018600] (Chemotaxis)	Chemotaxis
Khan Z, Michalopoulos GK, Stolz DB. Peroxisomal localization of hypoxia-inducible factors and hypoxia-inducible factor regulatory hydroxylases in primary rat hepatocytes exposed to hypoxia-reoxygenation. Am J Pathol 2006-10-01 [PMID: 17003483] (Rat)		Rat
Carroll, P A et al. Latent Kaposi's sarcoma-associated herpesvirus infection of endothelial cells activates hypoxia-induced factors. J Virol;80(21):10802-12. 2006-11-01 [PMID: 16956952] (IHC-P, Human)	IHC-P	Human
Shinojima, T et al. Renal cancer cells lacking hypoxia inducible factor (HIF)-1alpha expression maintain vascular endothelial growth factor expression through HIF-2alpha. Carcinogenesis;28(3):529-36. 2007-03-01 [PMID: 16920734] (WB)	WB
Zheng X, Ruas JL, Cao R et al. Cell-type-specific regulation of degradation of hypoxia-inducible factor 1a: Role of subcellular compartmentalization. Mol Cell Biol 2006-06-01 [PMID: 16738327]
Kim CM, Vocke C, Torres-Cabala C et al. Expression of hypoxia inducible factor-1alpha and 2alpha in genetically distinct early renal cortical tumors. J Urol 2006-05-01 [PMID: 16600797] (Human)		Human
Nakamura E, Abreu-e-Lima P, Awakura Y, Inoue T, Kamoto T, Ogawa O, Kotani H, Manabe T, Zhang GJ, Kondo K, Nose V, Kaelin WG Jr. Clusterin is a secreted marker for a hypoxia-inducible factor-independent function of the von Hippel-Lindau tumor suppressor protein. Am J Pathol;168(2):574-84. 2006-02-01 [PMID: 16436671]
Bastian Eul, Frank Rose,Stefanie Krick. Impact of HIF-1#945; HIF-2#945; on proliferation migration of human pulmonary artery fibroblasts in hypoxia. The FASEB Journal express article 10.1096/fj.05-4104fje. [PMID: 16263938]
Li, L et al. Evaluating Hypoxia-Inducible Factors-1alpha as a Cancer Therapeutic Target via Inducible RNA Interference In vivo. American Association for Cancer Research 65, 7249-7258. 2005-01-01 [PMID: 16103076] (WB, IF/IHC)	WB, IF/IHC
Isaacs JS, Jung YJ, Mole DR et al. HIF overexpression correlates with biallelic loss of fumarate hydratase in renal cancer: novel role of fumarate in regulation of HIF stability. Cancer Cell 2005-08-01 [PMID: 16098467] (ELISA, Human)	ELISA	Human
Bilton R, Mazure N, Trottier E et al. Arrest-defective-1 protein, an acetyltransferase, does not alter stability of hypoxia-inducible factor (HIF)-1alpha and is not induced by hypoxia or HIF. J Biol Chem 2005-09-02 [PMID: 15994306] (WB, Human)	WB	Human
Yoshimura H, Dhar DK, Kohno H, Kubota H, Fujii T, Ueda S, Kinugasa S, Tachibana M, Nagasue N. Prognostic impact of hypoxia-inducible factors 1alpha and 2alpha in colorectal cancer patients: correlation with tumor angiogenesis and cyclooxygenase-2 expression. Clin Cancer Res;10(24):8554-60. 2004-12-15 [PMID: 15623639]
Rathmell WK, Hickey MM, Bezman NA, Chmielecki CA, Carraway NC, Simon MC. In vitro and in vivo models analyzing von Hippel-Lindau disease-specific mutations. Cancer Res;64(23):8595-603. 2004-12-01 [PMID: 15574766]
Grabmaier, K et al. Strict regulation of CAIXG250/MN by HIF-1 alpha in clear cell renal cell carcinoma. Oncogene;23(33):5624-31. 2004-07-22 [PMID: 15184875] (Human)		Human
Mekhail, K et al. HIF activation by pH-dependent nucleolar sequestration of VHL. Nature Cell Biology. 6: 642-647. 2004-01-01 [PMID: 15181450]
Harvey AJ, Kind KL, Pantaleon M, Armstrong DT, Thompson JG. Oxygen-regulated gene expression in bovine blastocysts. Biol Reprod;71(4):1108-19. 2004-10-01 [PMID: 15163614]
Koike, T et al. Hypoxia induces adhesion molecules on cancer cells: A missing link between Warburg effect induction of selectin-lig carbohydrates. PNAS. 101:8132-8137. 2004-01-01 [PMID: 15141079]
Tissot van Patot MC, Bendrick-Peart J, Beckey VE et al. Greater vascularity, lowered HIF-1/DNA binding, and elevated GSH as markers of adaptation to in vivo chronic hypoxia. Am J Physiol Lung Cell Mol Physiol 2004-09-01 [PMID: 15132953]
Pan Y, Oprysko PR, Asham AM, Koch CJ, Simon MC. p53 cannot be induced by hypoxia alone but responds to the hypoxic microenvironment. Oncogene;23(29):4975-83. 2004-06-24 [PMID: 15107830]
Zimmer, M et al. Inhibition of Hypoxia-Inducible Factor Is Sufficient for Growth Supression of VHL-/- Tumors. Molecular Cancer Research 2: 89-95. 2004-01-01 [PMID: 14985465] (WB)	WB
EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE 16 E7 IS SUFFICIENT TO SIGNIFICANTLY INCREASE EXPRESSSION OF ANGIOGENIC FACTORS BUT IS NOT SUFFICIENT TO INDUCE ENDOTHELIAL CELL MIGRATION. Walker JA Oncogene [PMID: 14968115]
Kondo K, Kim WY, Lechpammer M, Kaelin WG Jr. Inhibition of HIF2alpha is sufficient to suppress pVHL-defective tumor growth. PLoS Biol 2003-12-01 [PMID: 14691554]
Martin-Puig S, Temes E, Olmos G, Jones DR, Aragones J, Landazuri MO. Role of iron (II)-2-oxoglutarate-dependent dioxygenases in the generation of hypoxia-induced phosphatidic acid through HIF-1/2 and von Hippel-Lindau-independent mechanisms. J Biol Chem;279(10):9504-11. 2004-03-05 [PMID: 14681229]
Mohan, S et al. von Hippel-Linau protein complex is regulated by cell density. Oncogene 22: 5270-5280. 2003-01-01 [PMID: 12917628] (WB)	WB
Xia G et al. (2002) Positive expression of HIF-2alpha/ EPAS1 in invasive bladder cancer. Urology;59(5):774-8. 2002-05-01 [PMID: 11992927]
Beasley NJ, Leek R, Alam M et al. Hypoxia-inducible factors HIF-1alpha and HIF-2alpha in head and neck cancer: relationship to tumor biology and treatment outcome in surgically resected patients. Cancer Res;62(9):2493-7. 2002-05-01 [PMID: 11980639] (IHC-P, Human)	IHC-P	Human
Giatromanolaki A, Koukourakis MI, Sivridis E et al. Expression of hypoxia-inducible carbonic anhydrase-9 relates to angiogenic pathways and independently to poor outcome in non-small cell lung cancer. Cancer Res;61(21):7992-8. 2001-11-01 [PMID: 11691824] (IHC-P, Human)	IHC-P	Human
Koukourakis MI, Giatromanolaki A, Skarlatos J et al. Hypoxia inducible factor (HIF-1a and HIF-2a) expression in early esophageal cancer and response to photodynamic therapy and radiotherapy. Cancer Res;61(5):1830-2. 2001-03-01 [PMID: 11280732] (IHC-P, Human)	IHC-P	Human
Blancher, C et al. Cancer Research. 60: 7106-7113. 2000-01-01 [PMID: 11156418] (WB, IF/IHC)	WB, IF/IHC
Talks, KL Turley, H Amer. J. of Pathology. 157: 411-421. 2000-01-01 [PMID: 10934146] (WB, IF/IHC)	WB, IF/IHC
Wiesener MS, Turley H, Allen WE, Willam C, Eckardt KU, Talks KL, Wood SM, Gatter KC, Harris AL, Pugh CW, Ratcliffe PJ, Maxwell PH. Induction of endothelial PAS domain protein-1 by hypoxia: characterization and comparison with hypoxia-inducible factor-1alpha. Blood;92(7):2260-8. 1998-10-01 [PMID: 9746763]
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Array NB100-132

• HIF-2 alpha/EPAS1 Antibodies
• HIF-2 alpha/EPAS1 Antibody Pack
• HIF-2 alpha/EPAS1 Lysate
• HIF-2 alpha/EPAS1 Proteins
• HIF-2 alpha/EPAS1 ELISA