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HA Tag Antibody (4810)

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 (3 Reviews)
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Immunocytochemistry/Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] diluted at 1:1000. Blue: Hoechst 33342 staining.
Immunocytochemistry/ Immunofluorescence: HA Tag Antibody (4810) [NBP2-43714] - HA-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HA stained by HA tag antibody [4810] ...read more
Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Human HEK293T cells overexpressed with Ly6K-HA. Dution 1:1000. Western blot image submitted by a verified customer review.
Flow Cytometry: HA Tag Antibody (4810) [NBP2-43714] - HEK293T cells overexpressed with Ly6K-HA stained with HA Epitope Tag Antibody (red) or secondary only (black). Flow cytometry image submitted from a verified ...read more
Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of 293T whole cell lysate/extract A: 20 ug whole cell lysate/extract of HA tagged protein expressing 293T cells B : Control with 2.5 ug of pre-immune mouse ...read more
Western Blot: HA Tag Antibody (4810) [NBP2-43714] - Analysis of A: 30 ug HeLa whole cell lysate/extract B: 30 ug whole cell lysate/extract of HA tagged protein expressing HeLa cells 5% SDS-PAGE HA tag antibody [4810] ...read more

Product Details

Summary
Reactivity ETSpecies Glossary
Applications WB, Flow, ICC/IF, IP
Clone
4810
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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HA Tag Antibody (4810) Summary

Immunogen
The immunogen used to generate this antibody corresponds to HA Tag
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry Valdiation from verified customer review
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:1000
  • Immunoprecipitation 1:100 - 1:500
  • Western Blot 1:1000 - 1:10000
Reviewed Applications
Read 3 Reviews rated 5
using
NBP2-43714 in the following applications:
Publications
Read Publications using NBP2-43714.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for HA Tag Antibody (4810)

  • HA Epitope Tag
  • HA Tag
  • HA
  • Hemagglutinin

Background

Human influenza hemagglutinin (HA) is a glycoprotein required for the infectivity of the human virus and is expressed as a homotrimer on the surface of the viral capsid (1). As a major antigen of the influenza virus, HA constantly evolves to escape herd immunity via antigenic shift which can lead to pandemics (2). The HA tag (YPYDVPDYA-tag) itself has a molecular weight of 1.1 kDa and is a linear epitope derived from amino acids 98-106 of the HA protein. It is used extensively as a general epitope tag in expression vectors (2, 3). The HA tag is frequently added to the N- or C- terminus of a protein of interest to facilitate protein purification, detection, and labeling using anti-HA antibodies (3, 4). HA Tag is cleaved by caspase 3/7 resulting in total loss of immunoreactivity, making it unsuitable for the study of apoptosis (5).

References

1. Wilks, S., Graaf, M. D., Smith, D. J., & Burke, D. F. (2012). A review of influenza haemagglutinin receptor binding as it relates to pandemic properties. Vaccine, 30(29), 4369-4376. doi:10.1016/j.vaccine.2012.02.076

2. Wu, N. C., & Wilson, I. A. (2019). Influenza hemagglutinin structures and antibody recognition. Cold Spring Harbor Perspectives in Medicine, 10(8). doi:10.1101/cshperspect.a038778

3. Zhao, X., Li, G., & Liang, S. (2013). Several affinity tags commonly used in chromatographic purification. Journal of Analytical Methods in Chemistry, 2013, 1-8. doi:10.1155/2013/581093

4. Kimple, M. E., Brill, A. L., & Pasker, R. L. (2013). Overview of affinity tags for protein purification. Current Protocols in Protein Science, 73(1). doi:10.1002/0471140864.ps0909s73

5. Schembri, L., Dalibart, R., Tomasello, F., Legembre, P., Ichas, F., & Giorgi, F. D. (2007). The HA tag is cleaved and loses immunoreactivity during apoptosis. Nature Methods, 4(2), 107-108. doi:10.1038/nmeth0207-107

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HA Tag Antibody (NBP2-43714)(4)

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(2)
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Reviews for HA Tag Antibody (NBP2-43714) (3) 53

Average Rating: 5
(Based on 3 reviews)
We have 3 reviews tested in 1 species: Human.

Reviews using NBP2-43714:
Filter by Applications
ICC
(1)
Flow
(1)
WB
(1)
All Applications
Filter by Species
Human
(3)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
 ICC Human 11/29/2021
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Summary

ApplicationImmunocytochemistry
Sample TestedHEK 293 cell line
SpeciesHuman
Lot4810
Flow Cytometry HA Tag NBP2-43714
Enlarge 		5
reviewed by:
Verified Customer
 Flow Human 07/06/2016
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman HEK293T cells overexoressed with Ly6K-HA
SpeciesHuman
Lot41246
Western Blot HA Tag NBP2-43714
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 06/17/2016
View

Summary

ApplicationWestern Blot
Sample Testedhuman HEK293T cells overexpressed with Ly6K-HA
SpeciesHuman
Lot41246

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HA Tag Antibody (NBP2-43714). (Showing 1 - 1 of 1 FAQs).

  1. We are looking for a NON-RABBIT polyclonal anti-HA (hemagglutinin - YPYDVPDYA ) antibody for immunohistochemistry that works in 4% paraformaldehyde-fixed tissue. We have a transgenic mouse expressing an HA-tagged protein and want to perform double-labeling with another antibody made in rabbit. We have had no luck using mouse or rat monoclonal anti-HA antibodies. The non-rabbit polyclonal antibody would preferably be made in goat, although if you have other species let us know. It could be unconjugated or conjugated (eg fluorochrome or HRP). However, we are only interested in a product with demonstrated effectiveness for IHC in a published paper. Do you have any such products?
    • We do have other host species for this target and some that have been mentioned in publications. Unfortunately for our goat antibodies against this tag none of them have been validated in IHC-P, and for two we have shown they do not work. Please see this link to our goat anti-HA Epitope Tag antibodies. Since they work in ICC/IF I would assume as long as the tag is expressed in your tissues that you should still pick it up. In this case we have an Innovators Reward Program you can take advantage of for testing a new application to save some money. Here are the chicken anti-HA Epitope Tag antibodies. None of them have been tested in IHC-P as before, or that would be indicated with a positive result and the application on the datasheet or a negative results with the (-) beside the application. Another option for you as well would be going with a Rabbit polyclonal that we have available that has been well publicized and reviewed by customers (cat# NB600-363). I know you plan on double staining, but one way around this would be to use your other antibody and incubate with the secondary for detection first. After doing that you could have this one directly conjugated to your preferred detection probe and then pick up both of their signals since you would already have bound secondary to your first rabbit primary, there would not be any non-specific binding.

Secondary Antibodies

 

Isotype Controls

Additional HA Tag Products

Array NBP2-43714

Research Areas for HA Tag Antibody (NBP2-43714)

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Blogs on HA Tag.

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Recent Reviews

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Immunocytochemistry HA Tag NBP2-43714
Enlarge
 Verified Customer
11/29/2021
Application: ICC
Species: Human
read this review
Flow Cytometry HA Tag NBP2-43714
Enlarge
 Verified Customer
07/06/2016
Application: Flow
Species: Human
read this review
Western Blot HA Tag NBP2-43714
Enlarge
 Verified Customer
06/17/2016
Application: WB
Species: Human
read this review