H/M Pluripotent Stem Cell Flow Cytometry Kit Summary
| Description |
This product is designed for the flow cytometric analysis of human/mouse PSCs using four fluorochrome-conjugated antibodies. Features- Verifies stem cell pluripotency
- Defines starting population to reduce variability
- Simultaneously detects four markers of pluripotency
- Does not require the use of secondary antibodies
|
| Kit Type |
Flow Cytometry Kit |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
Kit Components
|
Components
|
- 250 uL of Mouse IgG2A-PE Isotype Control
- 250 uL of Mouse IgG3-CFS Isotype Control
- 250 uL of Mouse IgM-PerCP Isotype Control
- 250 uL of Rat IgG2B-APC Isotype Control
- 30 mL of 1X Fixation/Permeabilization Buffer
- 30 mL of 1X Permeabilization/Wash Buffer
- Marker (Positive for human; Negative for mouse): 250 uL of SSEA-4-CFS Mouse IgG3; Clone MC-813-70
- Marker (Positive for mouse; Negative for human): 250 uL of SSEA-1-PerCP Mouse IgM; Clone MC-480
- Positive Marker: 250 uL of Oct-3/4-APC Rat IgG2B; Clone 240408
- Positive Marker: 250 uL of SOX2-PE Mouse IgG2A; Clone 245610
|
Background
Embryonic stem (ES) cells are pluripotent stem cells derived from the inner cell mass of pre-implantation embryos. Induced pluripotent stem (iPS) cells can be generated by somatic cell reprogramming following the exogenous expression of specific transcription factors (Oct-3/4, KLF4, SOX2, and c-Myc). These cell types are capable of unlimited, undifferentiated proliferation in vitro and still maintain the capacity to differentiate into a wide variety of somatic cells. In this capacity, pluripotent stem cells have widespread clinical potential for the treatments of heart disease, diabetes, spinal cord injury, and a variety of neurodegenerative disorders.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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Blogs on H/M Pluripotent Stem Cell
![Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - Mouse D3 embryonic stem cells were stained using reagents included in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit. Cells were analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometric analysis shows that 91.1% of mouse D3 embryonic stem cells are positive for both Oct-3/4 and SSEA1 expression. B. Flow cytometric analysis data shows that 82.6% of mouse D3 embryonic stem cells are positive for SSEA-1 and negative for SSEA-4 a phenotype consistent with mouse embryonic stem cells. C. Flow cytometric analysis shows that mouse D3 embryonic stem cells express the pluripotent marker SOX2.](http://images.novusbio.com/fullsize/H-M-Pluripotent-Stem-Cell-Flow-Cytometry-Kit-Flow-Cytometry-FMC001-NOV-img0003.jpg "Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - Mouse D3 embryonic stem cells were stained using reagents included in the Human/Mouse Embryonic Stem Cell Multi-Color Flow Cytometry Kit. Cells were analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometric analysis shows that 91.1% of mouse D3 embryonic stem cells are positive for both Oct-3/4 and SSEA1 expression. B. Flow cytometric analysis data shows that 82.6% of mouse D3 embryonic stem cells are positive for SSEA-1 and negative for SSEA-4 a phenotype consistent with mouse embryonic stem cells. C. Flow cytometric analysis shows that mouse D3 embryonic stem cells express the pluripotent marker SOX2.")
![Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - BG01V human embryonic stem cells were stained using reagents included in the Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit. Cells were simultaneously analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometry data shows that 91.9% of BG01V human embryonic stem cells are positive for both Oct-3/4 and SSEA4 expression. B. Flow cytometry data shows that 88.5% of BG01V human embryonic stem cells are positive for SSEA-4 and negative for SSEA-1, a phenotype consistent with human embryonic stem cells. C. Flow cytometric analysis shows that BG01V human embryonic stem cells express the pluripotent marker SOX2.](http://images.novusbio.com/fullsize/H-M-Pluripotent-Stem-Cell-Flow-Cytometry-Kit-Flow-Cytometry-FMC001-NOV-img0004.jpg "Flow Cytometry: H/M Pluripotent Stem Cell Flow Cytometry Kit [FMC001-NOV] - BG01V human embryonic stem cells were stained using reagents included in the Human/Mouse Pluripotent Stem Cell Multi-Color Flow Cytometry Kit. Cells were simultaneously analyzed for expression of pluripotent markers including SSEA-1, SSEA-4, Oct-3/4, and SOX2 by flow cytometry. A. Flow cytometry data shows that 91.9% of BG01V human embryonic stem cells are positive for both Oct-3/4 and SSEA4 expression. B. Flow cytometry data shows that 88.5% of BG01V human embryonic stem cells are positive for SSEA-4 and negative for SSEA-1, a phenotype consistent with human embryonic stem cells. C. Flow cytometric analysis shows that BG01V human embryonic stem cells express the pluripotent marker SOX2.")
