GFP Antibody

Chicken products cannot be exported to Canada.

Purification Notes
Chickens were immunized with purified recombinant green fluorescent protein (GFP) emulsified in Freund's adjuvant. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a filter-sterilized mixture of both affinity-purified antibodies (30 ug/ml) and purified IgY (10 mg/mL).

Storage Notes
Store at -20C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Since 50% glycerol is present in the vial, this antibody preparation should remain a liquid at -20C. For longer storage periods, store at -80C, but be aware that freezing this preparation may reduce its activity.

Green fluorescent protein (GFP) is a 27 kDa protein derived from the bioluminescent jellyfish Aquorea Victoria. GFP can be excited by light in the blue to ultraviolet spectrum and has an excitation peak at 395 nm with an emission peak at 509 nm in the green spectrum. GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. GFP fluorescence is stable under fixation conditions and antibodies are suitable for a variety of applications including ELISA, ICC/IF, IHC, IP and WB (1-3).

References
1. Shi, C., Pan, F. C., Kim, J. N., Washington, M. K., Padmanabhan, C., Meyer, C. T., . . . Means, A. L. (2019). Differential Cell Susceptibilities to Kras(G12D) in the Setting of Obstructive Chronic Pancreatitis. Cell Mol Gastroenterol Hepatol. doi:10.1016/j.jcmgh.2019.07.001

2. Zhao, S., Fortier, T. M., & Baehrecke, E. H. (2018). Autophagy Promotes Tumor-like Stem Cell Niche Occupancy. Curr Biol, 28(19), 3056-3064.e3053. doi:10.1016/j.cub.2018.07.075

3. Zusso, M., Lunardi, V., Franceschini, D., Pagetta, A., Lo, R., Stifani, S., . . . Moro, S. (2019). Ciprofloxacin and levofloxacin attenuate microglia inflammatory response via TLR4/NF-kB pathway. J Neuroinflammation, 16(1), 148. doi:10.1186/s12974-019-1538-9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 9 confirmed species: Human, Mouse, Rat, Drosophila, Firefly, Jellyfish, Primate, Yeast, Zebrafish.

We have publications tested in 11 applications: ICC/IF, IF/IHC, IHC, IHC-Fr, IHC-FrFl, IHC-P, IHC/IF, Immunohistochemistry, Immunohistochemistry-Frozen, In Vivo, WB.

Showing Publications 1 - 10 of 79. Show All 79 Publications. Collapse Publications.

Publications using NB100-1614	Applications	Species
Jorge A. Moreno, Olga Dudchenko, Charles Y. Feigin, Sarah A. Mereby, Zhuoxin Chen, Raul Ramos, Axel A. Almet, Harsha Sen, Benjamin J. Brack, Matthew R. Johnson, Sha Li, Wei Wang, Jenna M. Gaska, Alexander Ploss, David Weisz, Arina D. Omer, Weijie Yao, Zane Colaric, Parwinder Kaur, Judy St. Leger, Qing Nie, Alexandria Mena, Joseph P. Flanagan, Greta Keller, Thomas Sanger, Bruce Ostrow, Maksim V. Plikus, Evgeny Z. Kvon, Erez Lieberman Aiden, Ricardo Mallarino Emx2 underlies the development and evolution of marsupial gliding membranes Nature 2024-04-24 [PMID: 38658750]
Pisano T, Dhanerawala Z, Kislin M et al. Parallel organization of cerebellar pathways to sensorimotor, associative, and modulatory forebrain bioRxiv 2020-03-08
Kenji Yoshida, Daisuke Kato, Shouta Sugio, Ikuko Takeda, Hiroaki Wake Activity-dependent oligodendrocyte calcium dynamics and their changes in Alzheimer’s disease Frontiers in Cellular Neuroscience 2023-10-31 [PMID: 38026691]
Patil MJ, Kim SH, Bahia PK et al. A novel Flp reporter mouse shows that TRPA1 expression is largely limited to sensory neuron subsets eNeuro 2023-11-16 [PMID: 37989590]
Yost SA, Firlar E, Glenn JD et al. Characterization and biodistribution of under-employed gene therapy vector AAV7 Journal of virology 2023-10-16 [PMID: 37843374] (IHC-Fr) Details: Dilution 1:1000	IHC-Fr
Johnson MR, Li S, Guerrero-Juarez CF et al. A multifunctional Wnt regulator underlies the evolution of rodent stripe patterns Nature ecology & evolution 2023-10-09 [PMID: 37813945]
Tian W, Qin S, Zhang B et Al. T606-phosphorylated Kaiso interacting with 14-3-3 and p120ctn is sequestered in the cytoplasm of human cells bioRxiv 2020-01-01 (WB, Human)	WB	Human
Brandebura AN, Asbell QN, Micael MKB, Allen NJ Dysregulation of astrocyte-secreted pleiotrophin contributes to neuronal structural and functional phenotypes in Down Syndrome bioRxiv : the preprint server for biology 2023-09-27 [PMID: 37808668] (IHC-P) Details: 1:500 IHC-P diltuion	IHC-P
Babey ME, Krause WC, Herber CB et al. Brain-Derived CCN3 Is An Osteoanabolic Hormone That Sustains Bone in Lactating Females bioRxiv : the preprint server for biology 2023-08-29 [PMID: 37693376] (IHC-Fr)	IHC-Fr
Zhang T, Xu Z, Zheng D et al. Novel biallelic variants in the PLEC gene are associated with severe hearing loss Hearing research 2023-06-16 [PMID: 37393735] (WB)	WB
Boileau C, Deforges S, Peret A et al. GluK2 is a target for gene therapy in drug-resistant Temporal Lobe Epilepsy bioRxiv 2023-04-17 [PMID: 37341588]
Vijayakumar S, DiGuiseppi JA, Dabestani J et al. In Silico Transcriptome-based Screens Identify Epidermal Growth Factor Receptor Inhibitors as Therapeutics for Noise-induced Hearing Loss bioRxiv : the preprint server for biology 2023-06-09 [PMID: 37333346]
Evangelista B, Cahalan S, Ragusa J et al. Tandem detergent-extraction and immunoprecipitation of proteinopathy: Scalable enrichment of ALS-associated TDP-43 aggregates iScience 2023-05-01 [PMID: 37182104] (WB)	WB
Hashimoto A, Kawamura N, Tarusawa E et al. Microglia Enable Cross-Modal Plasticity by Removing Inhibitory Synapses SSRN Electronic Journal 2022-10-04 [PMID: 37086724]
Yang G, Ullah HMA, Parker E et al. Neurite outgrowth deficits caused by rare PLXNB1 mutation in pediatric bipolar disorder Molecular psychiatry 2023-04-10 [PMID: 37032361]
Bery A, Etienne O, Mouton L et al. XLF/Cernunnos loss impairs mouse brain development by altering symmetric proliferative divisions of neural progenitors Cell reports 2023-04-05 [PMID: 37027298] (WB)	WB
Hollidge BS, Carroll HB, Qian R et al. Kinetics and durability of transgene expression after intrastriatal injection of AAV9 vectors Frontiers in Neurology 2022-11-14 [PMID: 36452163] (Immunohistochemistry)	Immunohistochemistry
Ghochani Y, Muthukrishnan SD, Sohrabi A et al. A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration Cell reports 2022-10-18 [PMID: 36261010]
Wang L, Park JY, Liu F et al. A kinase-independent function of cyclin-dependent kinase 6 promotes outer radial glia expansion and neocortical folding Proceedings of the National Academy of Sciences 2022-09-20 [PMID: 36095192] (Immunohistochemistry-Frozen)	Immunohistochemistry-Frozen
Zhou P, Zhang H, Wang Y et al. C-kit+VEGFR-2+ Mesenchymal Stem Cells Differentiate into Cardiovascular Cells and Repair Infarcted Myocardium after Transplantation Stem Cell Rev Rep 2022-08-13 [PMID: 35962935]
Tian W, Yuan H, Qin S et al. Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1 Molecular oncology 2022-07-19 [PMID: 35851744] (WB)	WB
Kann AP, Hung M, Wang W et al. An injury-responsive Rac-to-Rho GTPase switch drives activation of muscle stem cells through rapid cytoskeletal remodeling Cell stem cell 2022-05-15 [PMID: 35597234] (ICC/IF) Details: Fig. 4L Muscle stem cells (SCs) were isolated from the hindlimb muscles of Pax7nGFP mice	ICC/IF
Ando K, Tong L, Peng D et al. KCNJ8/ABCC9-containing K-ATP channel modulates brain vascular smooth muscle development and neurovascular coupling Developmental cell 2022-05-11 [PMID: 35588738]
Martin Hsu, Collin Laaker, Andy Madrid, Melinda Herbath, Yun Hwa Choi, Matyas Sandor et al. Neuroinflammation creates an immune regulatory niche at the meningeal lymphatic vasculature near the cribriform plate Nature Immunology 2022-04-01 [PMID: 35347285]
Komachi K, Burgess SM The Nup2 meiotic-autonomous region relieves inhibition of Nup60 to promote progression of meiosis and sporulation in Saccharomyces cerevisiae Genetics 2022-03-18 [PMID: 35302609] (ICC/IF)	ICC/IF
Lloyd-Lewis B, Gobbo F, Perkins M et al. In vivo imaging of mammary epithelial cell dynamics in response to lineage-biased Wnt/beta-catenin activation Cell reports 2022-03-08 [PMID: 35263603] (IF/IHC)	IF/IHC
Borriello L, Coste A, Traub B et al. Primary tumor associated macrophages activate programs of invasion and dormancy in disseminating tumor cells Nature communications 2022-02-02 [PMID: 35110548] (IHC-P, ICC/IF)	IHC-P, ICC/IF
Sharma VP, Tang B, Wang Y et al. Live tumor imaging shows macrophage induction and TMEM-mediated enrichment of cancer stem cells during metastatic dissemination Nature communications 2021-12-15 [PMID: 34911937] (In Vivo, ICC/IF)	In Vivo, ICC/IF
Lee M, Nagashima K, Yoon J et al. CEP97 phosphorylation by Dyrk1a is critical for centriole separation during multiciliogenesis Journal of Cell Biology 2022-01-03 [PMID: 34787650]
Joseph NF, Zucca A, Wingfield JL Et al. Molecular motor KIF3B in the prelimbic cortex constrains the consolidation of contextual fear memory Molecular brain 2021-11-08 [PMID: 34749771] (IHC-Fr)	IHC-Fr
Kamm GB, Boffi JC, Zuza K Et al. A synaptic temperature sensor for body cooling Neuron 2021-10-20 [PMID: 34672983]
Hollon N, Williams E, Howard C, et al. Nigrostriatal Dopamine Signals Sequence-Specific Action-Outcome Prediction Errors Curr Biol 2021-10-12 [PMID: 34637751]
Ehrlich A, Semache M, Couvineau P et al. Ackr3-Venus knock-in mouse lights up brain vasculature Molecular Brain 2021-12-01 [PMID: 34583741] (IF/IHC)	IF/IHC
Pisano T, Dhanerawala Z, Kislin M et al. Parallel Organization of Cerebellar Pathways to Sensory, Motor, and Associative Forebrain SSRN Electronic Journal 2021-03-26 [PMID: 34551311]
Pradhan G, Wu C, Villarreal D et al. Beta Cell GHS-R Regulates Insulin Secretion and Sensitivity International Journal of Molecular Sciences 2021-04-11 [PMID: 33920473] (IF/IHC)	IF/IHC
Perniss A, Schmidt P, Soultanova A et al. Development of epithelial cholinergic chemosensory cells of the urethra and trachea of mice Cell and tissue research 2021-02-22 [PMID: 33616728] (Mouse)		Mouse
Chiola S, Napan KL, Wang Y et al. Defective AMPA-mediated synaptic transmission and morphology in human neurons with hemizygous SHANK3 deletion engrafted in mouse prefrontal cortex Molecular psychiatry 2021-02-08 [PMID: 33558651] (IHC-FrFl, Mouse)	IHC-FrFl	Mouse
Zallocchi M, Hati S, Xu Z, et al. Characterization of quinoxaline derivatives for protection against iatrogenic-induced hearing loss JCI insight 2021-01-21 [PMID: 33476306] (ICC/IF)	ICC/IF
Ruppert AL, Keshavarz M, Winterberg S et al. Advillin is a tuft cell marker in the mouse alimentary tract Journal of Molecular Histology 2020-08-01 [PMID: 32617896]
Bailly J, Del Rossi N et al. Targeting Morphine-Responsive Neurons: Generation of a Knock-In Mouse Line Expressing Cre Recombinase from the Mu-Opioid Receptor Gene Locus. Eneuro 2020-10-05 [PMID: 32381649] (IF/IHC)	IF/IHC
Wang L, Tang Q, Xu J Et Al. The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice J. Biol. Chem. 2020-03-13 [PMID: 32169905] (IF/IHC)	IF/IHC
Xu J, Wang L, Li H et al. Short stature homeobox 2 (SHOX2) regulates osteogenic differentiation and pattern formation during hard palate development in mice J. Biol. Chem. 2019-10-24 [PMID: 31649032] (IF/IHC, IHC-P, Mouse)	IF/IHC, IHC-P	Mouse
Ehrlich A T, Semache M et al. Biased Signaling of the Mu Opioid Receptor Revealed in Native Neurons. iScience 2019-03-15 [PMID: 30925410] (ICC/IF)	ICC/IF
Sanchez SMR, Fuson O, Tarang S et al. Quinoxaline protects zebrafish lateral line hair cells from cisplatin and aminoglycosides damage. Sci Rep. 2018-10-11 [PMID: 30310154] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Lean GA, Liu YJ, Lyon DC. Cell type specific tracing of the subcortical input to primary visual cortex from the basal forebrain. J. Comp. Neurol. 2018-02-14 [PMID: 29441578] (IF/IHC)	IF/IHC
Liu Y, Cromeens BP, Wang Y, Fisher K. Comparison of Different in vivo Incubation Sites to Produce Tissue Engineered Small Intestine. Tissue Eng Part A. 2018-01-31 [PMID: 29383981]
Bailly J Mu opioid receptor-positive neurons in habenular networks: aversive states Thesis 2022-01-01 (IHC-Fr)	IHC-Fr
Madsen SD, Jones SH, Tucker HA et al. Survival of aging CD264+ and CD264- populations of human bone marrow mesenchymal stem cells is independent of colony-forming efficiency Biotechnol. Bioeng. 2019-10-15 [PMID: 31612990]
Laura Padrón-Barthe, María Villalba-Orero, Jesús M. Gómez-Salinero, Fernando Domínguez, Marta Román, Javier Larrasa-Alonso, Paula Ortiz-Sánchez, Fernando Martínez, Marina López-Olañeta, Elena Bonzón-Kulichenko, Jesús Vázquez, Carlos Martí-Gómez, Demetrio J. Santiago, Belén Prados, Giovanna Giovinazzo, María Victoria Gómez-Gaviro, Silvia Priori, Pablo Garcia-Pavia, Enrique Lara-Pezzi Severe Cardiac Dysfunction and Death Caused by Arrhythmogenic Right Ventricular Cardiomyopathy Type 5 Are Improved by Inhibition of Glycogen Synthase Kinase-3 beta Circulation 2019-10-01 [PMID: 31567019]
Aoki S, Smith JB, Li H et al. An open cortico-basal ganglia loop allows limbic control over motor output via the nigrothalamic pathway Elife 2019-09-06 [PMID: 31490123] (IHC/IF, Rat)	IHC/IF	Rat
Herber C, Krause W, Wang L et al. Estrogen Signaling in Arcuate Kiss1 Neurons Suppresses a Sex-Dependent Circuit That Promotes Dense Strong Bones in Female Mice Nat Commun 2019-01-13 [PMID: 30635563] (IF/IHC, Jellyfish)	IF/IHC	Jellyfish
Xuan X, Gao F, Ma X et al. Activation of ACE2/angiotensin (1-7) attenuates pancreatic B cell dedifferentiation in a high-fat-diet mouse model. Metab. Clin. Exp. 2017-12-07 [PMID: 29225087] (Mouse)		Mouse
Goodman L, Zallocchi M Integrin alpha 8 and Pcdh15 act as a complex to regulate cilia biogenesis in sensory cells J Cell Sci. 2017-10-31 [PMID: 28883094] (ICC/IF, Zebrafish)	ICC/IF	Zebrafish
Mullen BR, Ross B, Chou JW et al. A Complex Interaction Between Reduced Reelin Expression and Prenatal Organophosphate Exposure Alters Neuronal Cell Morphology ASN Neuro 2016-06-01 [PMID: 27364165] (ICC/IF, Jellyfish)	ICC/IF	Jellyfish
Feng L, Wang Y, Cai H et al. ADAM10-Notch signaling governs the recruitment of ovarian pregranulosa cells and controls folliculogenesis in mice. J. Cell. Sci. 2016-04-15 [PMID: 27084580] (IHC-P)	IHC-P
Klein C, Rasinska J, Empl L et al. Physical exercise counteracts MPTP-induced changes in neural precursor cell proliferation in the hippocampus and restores spatial learning but not memory performance in the water maze. Behav Brain Res 2016-07-01 [PMID: 27012392]
Sorrentino NC, Maffia V, Strollo S et al. A Comprehensive Map of CNS Transduction by Eight Recombinant Adeno-associated Virus Serotypes Upon Cerebrospinal Fluid Administration in Pigs. Mol. Ther. 2015-12-07 [PMID: 26639405] (IHC-Fr)	IHC-Fr
Wiederhold S, Papadakis T, Chubanov V et al. A novel cholinergic epithelial cell with chemosensory traits in the murine conjunctiva Int. Immunopharmacol. 2015-06-25 [PMID: 26119492] (IHC-Fr)	IHC-Fr
Krasteva-Christ G, Soultanova A, Schutz B et al. Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts. Int Immunopharmacol 2015-05-29 [PMID: 26033492] (Mouse)		Mouse
Yang Y, Tsuchiya D, Lacefield S. Bub3 promotes Cdc20-dependent activation of the APC/C in S. cerevisiae. J. Cell Biol. 2015-05-25 [PMID: 25987604] (ICC/IF, Yeast)	ICC/IF	Yeast
Veres TZ, Voedisch S, Spies E et al. Aeroallergen Challenge Promotes Dendritic Cell Proliferation in the Airways J Immunol 2012-12-24 [PMID: 23267021] (ICC/IF, IF/IHC, Mouse)	ICC/IF, IF/IHC	Mouse
Yu L, Han M, Yan M et al. BMP2 induces segment-specific skeletal regeneration from digit and limb amputations by establishing a new endochondral ossification center Dev Biol 2012-10-03 [PMID: 23041115] (IF/IHC, ICC/IF, Mouse)	IF/IHC, ICC/IF	Mouse
Zallocchi M, Delimont D, Meehan DT, Cosgrove D. Regulated Vesicular Trafficking of Specific PCDH15 and VLGR1 Variants in Auditory Hair Cells J Neurosci 2012-10-03 [PMID: 23035094] (ICC/IF, Mouse)	ICC/IF	Mouse
Chiola S, Yang J, Ullah H et al. Transcriptional changes consistent with impaired neuronal differentiation, angiogenesis, and tumor plasticity induced in human subpallial telencephalic organoid-glioblastoma chimeras bioRxiv 2023-05-12 (IHC)	IHC
Veres TZ, Shevchenko M, Krasteva G et al. Dendritic Cell-Nerve Clusters Are Sites of T Cell Proliferation in Allergic Airway Inflammation. Am J Pathol;174(3):808-817. 2009-01-01 [PMID: 19179611]
Haas S, Bravo F, Ionescu T et al. Active Suppression of the Nigrostriatal Pathway during Optogenetic Stimulation Revealed by Simultaneous fPET/fMRI bioRxiv 2023-10-20 (IHC-P)	IHC-P
Raza QS, Vanderploeg JL, Jacobs JR. Matrix Metalloproteinases are required for membrane motility and lumenogenesis during Drosophila heart development. PLoS ONE. 2017-02-13 [PMID: 28192468] (IF/IHC, Drosophila)	IF/IHC	Drosophila
Neri S, Ishii G, Hashimoto H et al. Podoplanin-expressing cancer-associated fibroblasts lead and enhance the local invasion of cancer cells in lung adenocarcinoma Int. J. Cancer. 2015-02-03 [PMID: 25648219] (IHC-P)	IHC-P
Gorospe KA Investigating the Role of the LINC Complex on Skeletal Muscle Stem and Progenitor Cell Expansion Thesis 2023-01-01
Ehrlich AT, Semache M, Bailly J et al. Mapping GPR88-Venus illuminates a novel role for GPR88 in sensory processing Brain Struct Funct. 2017-11-06 [PMID: 29110094] (Mouse)		Mouse
Ogawa Y, Kanda T, Vogt K, Yanagisawa, M. Anatomical and Electrophysiological Development of the Hypothalamic Orexin Neurons from Embryos to Neonates. J. Comp. Neurol. 2017-06-12 [PMID: 28608460]
Choi J, Clement K, Huebner AJ et al. DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells. Cell Stem Cell. 2017-03-29 [PMID: 28366588]
Achariyar T M, Li B et al. Glymphatic distribution of CSF-derived apoE into brain is isoform specific and suppressed during sleep deprivation. Mol Neurodegener 2016-08-12 [PMID: 27931262] (IF/IHC)	IF/IHC
Panneck Ar, Rafiq A, Schutz B et al. Cholinergic epithelial cell with chemosensory traits in murine thymic medulla. Cell Tissue Res. 2014-10-10 [PMID: 25300645] (IF/IHC, Mouse)	IF/IHC	Mouse
Haq W, Arango-Gonzalez B, Zrenner E. Synaptic Remodeling Generates Synchronous Oscillations in the Degenerated Outer Mouse Retina. Frontiers in Neural Circuits. 2014-08-19 [PMID: 25249942] (IF/IHC, Mouse)	IF/IHC	Mouse
Klein C, Hain EG, Braun J et al. Enhanced adult neurogenesis increases brain stiffness: in vivo magnetic resonance elastography in a mouse model of dopamine depletion. PLoS ONE 2014-03-26 [PMID: 24667730] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Lee J, Marrero L, Yu L et al. SDF-1 alpha/CXCR4 signaling mediates digit tip regeneration promoted by BMP-2 Dev Biol 2013-08-07 [PMID: 23916851] (IF/IHC, ICC/IF, Primate)	IF/IHC, ICC/IF	Primate
Canty AJ, Huang L, Jackson JS et al. In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits. Nat Commun 2013-06-25 [PMID: 23799397] (IHC-Fr, ICC/IF, Firefly)	IHC-Fr, ICC/IF	Firefly
Wolff M, Noreikat K, Ibanez-Tallon I et al. Cholinergic receptors in the murine oviduct: Inventory and coupling to intracellular calcium concentration Life Sciences 2012-03-12 [PMID: 22480510] (IF/IHC, Mouse)	IF/IHC	Mouse
Show All 79 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
	5 reviewed by: Verified Customer	IHC-Fr	Mouse	04/05/2024	View Summary Application Immunohistochemistry-Frozen Sample Tested Sciatic Nerve Species Mouse Lot GFP917979 Comments Comments NB100-1614 was diluted 1 in 10000 and was left on tissue sections overnight at 4 degrees Celsius. Secondary antibody was donkey anti chicken conjugated to Alexa 647.
	5 reviewed by: Sergio Barrios	IHC	Mouse	10/04/2023	View Summary Application Immunohistochemistry Sample Tested Cancer Cells Species Mouse Lot GFP917979
Enlarge	4 reviewed by: Olivier Etienne	IHC-P	Mouse	02/17/2020	View Summary Application Immunohistochemistry-Paraffin Sample Tested embryonic mouse brain Species Mouse Lot GFP879484
Enlarge	4 reviewed by: Verified Customer	ICC	Human	05/09/2018	View Summary Application Immunocytochemistry Sample Tested LNCaP human prostate cancer cell line Species Human Lot B-2 Comments Comments Human LnCap cells were grown in RPMI1640 + 10% FCS in 5% CO2 at 37C. The cells were electroporated with GFP expressing plasmid using Amaxa Nucleofection kit.
Enlarge	5 reviewed by: Verified Customer	ICC	Human	12/25/2016	View Summary Application Immunocytochemistry Sample Tested hela cell Species Human Comments Comments GFP expressing cells were fixed with PFA 4% for 30 min, washed several times with PBS and then permeabilized for 5 min with PBSTx-100 1%. After several washes cells were incubated with the chicken anti-GFP 1:500 in fish blocking solution (PBS + 2.5% fish gelatin + 2.5% FCS) overnight at 4C. The next day after several washes with PBS, cells were incubated with anti-chicken Alexa 488 1:400 in fish gelatin for 1hour at room temperature.
Enlarge	4 reviewed by: Verified Customer	IF	Rat	02/17/2016	View Summary Application Immunofluorescence Sample Tested rat cortical culture (2 weeks old) Species Rat
Enlarge	5 reviewed by: Hongtian Yang	IF	Rat	08/23/2013	View Summary Application Immunofluorescence Sample Tested cultured hippocampal neuron from rat Species Rat Lot 0511FP12
Enlarge	4 reviewed by: Verified Customer	IHC	Mouse	05/15/2012	View Summary Application Immunohistochemistry Sample Tested Mouse Trigeminal Ganglia, Cornea Species Mouse Lot 0316FP11

Array NB100-1614

• GFP Antibodies
• GFP Proteins