![Western Blot: GFP Antibody (9F9.F9) [Biotin] [NB110-40670] - Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Biotin-Western-Blot-NB110-40670-img0008.jpg "Western Blot: GFP Antibody (9F9.F9) [Biotin] [NB110-40670] - Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti-GFP (MOUSE) Monoclonal Antibody Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kDa for GFP. Other band(s): none.")
| Reactivity | All-NASpecies Glossary |
| Applications | WB, ELISA, IHC, KD |
| Clone | 9F9.F9 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Biotin |
| Description | For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room GFP Biotin Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Mouse Serum |
| Immunogen | Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria. |
| Specificity | Reactivity is observed against recombinant Green Fluorescent Protein from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | This product is designed to detect enhanced GFP and GFP containing recombinant proteins. Tested in E, WB, IHC. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
| Preservative | 0.01% Sodium Azide |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry: GFP Antibody (9F9.F9) [Biotin] [NB110-40670] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915](http://images.novusbio.com/fullsize/GFP-Antibody-9F9-F9-Biotin-Immunohistochemistry-NB110-40670-img0004.jpg "Immunohistochemistry: GFP Antibody (9F9.F9) [Biotin] [NB110-40670] - Used 1:5000 As referenced in: Lu S-M, Tremblay M-E` , King IL, Qi J, Reynolds HM, et al. (2011) HIV-1 Tat-Induced Microgliosis and Synaptic Damage via Interactions between Peripheral and Central Myeloid Cells. PLoS ONE 6(9): e23915. doi:10.1371/journal.pone.0023915")
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-P3-6-2-8-1-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")