We see high background in our western blots using GAPDH antibodies H00002483-M01 and NB100-105.

Name: FRG1 Antibody (4A5)
Brand: Novus Biologicals
SKU: H00002483-M01
Price: 519 USD

Question

We see high background in our western blots using GAPDH antibodies H00002483-M01 and NB100-105.

Accepted Answer

To avoid multiple bands, we suggest:1)     Using a nitrocellulose membrane. It produces less background compared to PVDF membranes.2)     Overnight blocking at 4℃.3)     Increasing the wash time. Try washing the membrane for 10 mins, repeating 4 times.

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Reactivity	Hu, MuSpecies Glossary
Applications	WB, ELISA, ICC/IF, IP
Clone	4A5
Clonality	Monoclonal
Host	Mouse
Conjugate	Unconjugated

Description	Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen	FRG1 (AAH53997, 1 a.a. ~ 258 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEYSYVKSTKLVLKGTKTKSKKKKSKDKKRKREEDEETQLDIVGIWWTVTNFGEISGTIAIEMDKGTYIHALDNGLFTLGAPHKEVDEGPSPPEQFTAVKLSDSRIALKSGYGKYLGINSDGLVVGRSDAIGPREQWEPVFQNGKMALLASNSCFIRCNEAGDIEAKSKTAGEEEMIKIRSCAERETKKKDDIPEEDKGNVKQCEINYVKKFQSFQDHKLKISKEDSKILKKARKDGFLHETLLDRRAKLKADRYCK
Specificity	FRG1 - FSHD region gene 1
Isotype	IgG2a Kappa
Clonality	Monoclonal
Host	Mouse
Gene	FRG1
Purity	IgG purified
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Storage	Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer	In 1x PBS, pH 7.4
Preservative	No Preservative
Purity	IgG purified

Publications using H00002483-M01	Applications	Species
Tiwari A, Pattanaik N, Jaiswal AM, Dixit M. Increased FRG1 expression reduces in vitro cell migration, invasion and angiogenesis, ex vivo supported by reduced expression in tumors Biosci. Rep. 2017-09-25 [PMID: 28947680] (WB, Human)	WB	Human
Wallace LM, Garwick-Coppens SE, Tupler R, Harper SQ. RNA Interference Improves Myopathic Phenotypes in Mice Over-expressing FSHD Region Gene 1 (FRG1). Mol Ther. 2011-07-05 [PMID: 21730972]

FRG1 Antibody (4A5)

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Bioinformatics

Gene Symbol	FRG1
Entrez	2483(Human)
OMIM	601278(Human)
Uniprot	AAH53997(Human)