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Fluorescein Antibody [Alkaline Phosphatase]

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Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Alkaline Phosphatase

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Fluorescein Antibody [Alkaline Phosphatase] Summary

Description
Store vial at 4C before opening. DO NOT FREEZE. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Fluorescein Antibody Alkaline Phosphatase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, anti-Alkaline Phosphatase (calf intestine) and Fluorescein conjugated IgG.
Immunogen
Fluorescein conjugated to Goat IgG
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • ELISA 1:2000-1:10000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2500
Application Notes
This product has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, in situ hybridization and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Fluorescein Antibody [Alkaline Phosphatase]

  • FITC
  • fluorescein isothyocianate

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Fluorescein Products

Array NB600-969

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